Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus
Ubiquitin-conjugating enzyme (UBC) is a critical part of the ubiquitin–proteasome pathway and plays crucial roles in growth, development and abiotic stress response in plants. Although UBC genes have been detected in several plant species, characterization of this gene family at the whole-genome lev...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1118339/full |
| _version_ | 1797867255465246720 |
|---|---|
| author | Shengli Yao Meili Xie Ming Hu XiaoBo Cui Haoming Wu Xiaohua Li Peng Hu Chaobo Tong Xiaoli Yu |
| author_facet | Shengli Yao Meili Xie Ming Hu XiaoBo Cui Haoming Wu Xiaohua Li Peng Hu Chaobo Tong Xiaoli Yu |
| author_sort | Shengli Yao |
| collection | DOAJ |
| description | Ubiquitin-conjugating enzyme (UBC) is a critical part of the ubiquitin–proteasome pathway and plays crucial roles in growth, development and abiotic stress response in plants. Although UBC genes have been detected in several plant species, characterization of this gene family at the whole-genome level has not been conducted in Brassica napus. In the present study, 200 putative BnUBCs were identified in B. napus, which were clustered into 18 subgroups based on phylogenetic analysis. BnUBCs within each subgroup showed relatively conserved gene architectures and motifs. Moreover, the gene expression patterns in various tissues as well as the identification of cis-acting regulatory elements in BnUBC promoters suggested further investigation of their potential functions in plant growth and development. Furthermore, three BnUBCs were predicted as candidate genes for regulating agronomic traits related to oil content and yield through association mapping. In conclusion, this study provided a wealth of information on the UBC family in B. napus and revealed their effects on oil content and yield, which will aid future functional research and genetic breeding of B. napus. |
| first_indexed | 2024-04-09T23:37:21Z |
| format | Article |
| id | doaj.art-859ba1a915b541ed8b67623b332d8a48 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T23:37:21Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-859ba1a915b541ed8b67623b332d8a482023-03-20T05:15:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11183391118339Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napusShengli Yao0Meili Xie1Ming Hu2XiaoBo Cui3Haoming Wu4Xiaohua Li5Peng Hu6Chaobo Tong7Xiaoli Yu8School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, ChinaThe Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs of the PRC, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, ChinaThe Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs of the PRC, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, ChinaThe Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs of the PRC, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, ChinaSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, ChinaSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, ChinaSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, ChinaThe Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs of the PRC, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, ChinaSchool of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, ChinaUbiquitin-conjugating enzyme (UBC) is a critical part of the ubiquitin–proteasome pathway and plays crucial roles in growth, development and abiotic stress response in plants. Although UBC genes have been detected in several plant species, characterization of this gene family at the whole-genome level has not been conducted in Brassica napus. In the present study, 200 putative BnUBCs were identified in B. napus, which were clustered into 18 subgroups based on phylogenetic analysis. BnUBCs within each subgroup showed relatively conserved gene architectures and motifs. Moreover, the gene expression patterns in various tissues as well as the identification of cis-acting regulatory elements in BnUBC promoters suggested further investigation of their potential functions in plant growth and development. Furthermore, three BnUBCs were predicted as candidate genes for regulating agronomic traits related to oil content and yield through association mapping. In conclusion, this study provided a wealth of information on the UBC family in B. napus and revealed their effects on oil content and yield, which will aid future functional research and genetic breeding of B. napus.https://www.frontiersin.org/articles/10.3389/fpls.2023.1118339/fullBrassica napusthe UBC familyevolutionassociation mapping analysisoil content and yield |
| spellingShingle | Shengli Yao Meili Xie Ming Hu XiaoBo Cui Haoming Wu Xiaohua Li Peng Hu Chaobo Tong Xiaoli Yu Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus Frontiers in Plant Science Brassica napus the UBC family evolution association mapping analysis oil content and yield |
| title | Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus |
| title_full | Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus |
| title_fullStr | Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus |
| title_full_unstemmed | Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus |
| title_short | Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield of Brassica napus |
| title_sort | genome wide characterization of ubiquitin conjugating enzyme gene family explores its genetic effects on the oil content and yield of brassica napus |
| topic | Brassica napus the UBC family evolution association mapping analysis oil content and yield |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1118339/full |
| work_keys_str_mv | AT shengliyao genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT meilixie genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT minghu genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT xiaobocui genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT haomingwu genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT xiaohuali genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT penghu genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT chaobotong genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus AT xiaoliyu genomewidecharacterizationofubiquitinconjugatingenzymegenefamilyexploresitsgeneticeffectsontheoilcontentandyieldofbrassicanapus |