Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis
The improvement of grain yield, quality, and resistance can be achieved through the utilization of heterosis. The combination of cytoplasmic male sterility (CMS) and fertility restoration (<i>Rf</i>) gene(s) greatly facilitates the commercial development of three-line hybrid rice based o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/19/14832 |
| Summary: | The improvement of grain yield, quality, and resistance can be achieved through the utilization of heterosis. The combination of cytoplasmic male sterility (CMS) and fertility restoration (<i>Rf</i>) gene(s) greatly facilitates the commercial development of three-line hybrid rice based on heterosis. The basis for investigating the relationship between CMS and <i>Rf</i> genes lies in the rapid localization of wild rice fertility restoration genes. A set of the BC<sub>4</sub>F<sub>5</sub> population derived from interspecific crosses between Xieqingzao B (XB) and the BC<sub>1</sub>F<sub>9</sub> XB//Dongxiang wild rice (DWR)/XB line L5339 was used to detect quantitative trait loci (QTL) for fertility restoration. The population was then crossed with two male sterile lines, Zhong9A (Z9A) and DongB11A (DB11A), in order to generate a testcrossing population for investigating spikelet fertility. Based on the linkage mapping, seven QTLs were detected on chromosomes 1, 3, 5, 6, 8, and 10, explaining 2.76 to 12.46% of the phenotypic variation. Of them, two novel fertility restoration QTLs, <i>qRf3</i> and <i>qRf6</i>, can restore fertility of the CMS-DWR line DB11A by 16.56% and 15.12%, respectively. By employing joint QTL-seq and GradedPool-Seq methods, two novel <i>Rf</i> QTLs for DB11A, <i>qRf3</i> and <i>qRf6</i>, were identified at the physical locations of 10,900,001–11,700,000 bp and 28,016,785–31,247,556 bp, respectively. These findings are useful for exploring the natural variations of <i>Rf</i> genes in rice. Therefore, rice’s new genetic resources for the selection and breeding of rice restorer lines provide promising candidates for QTL fine localization and clarification. |
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| ISSN: | 1661-6596 1422-0067 |