Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis
The improvement of grain yield, quality, and resistance can be achieved through the utilization of heterosis. The combination of cytoplasmic male sterility (CMS) and fertility restoration (<i>Rf</i>) gene(s) greatly facilitates the commercial development of three-line hybrid rice based o...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/19/14832 |
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| author | Wenshan Cai Wanlin Li Liuying Duan Yaling Chen Fantao Zhang Biaolin Hu Jiankun Xie |
| author_facet | Wenshan Cai Wanlin Li Liuying Duan Yaling Chen Fantao Zhang Biaolin Hu Jiankun Xie |
| author_sort | Wenshan Cai |
| collection | DOAJ |
| description | The improvement of grain yield, quality, and resistance can be achieved through the utilization of heterosis. The combination of cytoplasmic male sterility (CMS) and fertility restoration (<i>Rf</i>) gene(s) greatly facilitates the commercial development of three-line hybrid rice based on heterosis. The basis for investigating the relationship between CMS and <i>Rf</i> genes lies in the rapid localization of wild rice fertility restoration genes. A set of the BC<sub>4</sub>F<sub>5</sub> population derived from interspecific crosses between Xieqingzao B (XB) and the BC<sub>1</sub>F<sub>9</sub> XB//Dongxiang wild rice (DWR)/XB line L5339 was used to detect quantitative trait loci (QTL) for fertility restoration. The population was then crossed with two male sterile lines, Zhong9A (Z9A) and DongB11A (DB11A), in order to generate a testcrossing population for investigating spikelet fertility. Based on the linkage mapping, seven QTLs were detected on chromosomes 1, 3, 5, 6, 8, and 10, explaining 2.76 to 12.46% of the phenotypic variation. Of them, two novel fertility restoration QTLs, <i>qRf3</i> and <i>qRf6</i>, can restore fertility of the CMS-DWR line DB11A by 16.56% and 15.12%, respectively. By employing joint QTL-seq and GradedPool-Seq methods, two novel <i>Rf</i> QTLs for DB11A, <i>qRf3</i> and <i>qRf6</i>, were identified at the physical locations of 10,900,001–11,700,000 bp and 28,016,785–31,247,556 bp, respectively. These findings are useful for exploring the natural variations of <i>Rf</i> genes in rice. Therefore, rice’s new genetic resources for the selection and breeding of rice restorer lines provide promising candidates for QTL fine localization and clarification. |
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| language | English |
| last_indexed | 2024-03-10T21:42:52Z |
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| spelling | doaj.art-859e0f14fbde43a59a726e70c9c727c72023-11-19T14:31:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124191483210.3390/ijms241914832Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation AnalysisWenshan Cai0Wanlin Li1Liuying Duan2Yaling Chen3Fantao Zhang4Biaolin Hu5Jiankun Xie6Laboratory of Plant Genetic Improvement and Biotechnology, Jiangxi Normal University, Nanchang 330200, ChinaLaboratory of Plant Genetic Improvement and Biotechnology, Jiangxi Normal University, Nanchang 330200, ChinaLaboratory of Plant Genetic Improvement and Biotechnology, Jiangxi Normal University, Nanchang 330200, ChinaLaboratory of Plant Genetic Improvement and Biotechnology, Jiangxi Normal University, Nanchang 330200, ChinaLaboratory of Plant Genetic Improvement and Biotechnology, Jiangxi Normal University, Nanchang 330200, ChinaRice Research Institute, National Engineering Laboratory for Rice (Nanchang), Jiangxi Academy of Agricultural Sciences, Nanchang 330200, ChinaLaboratory of Plant Genetic Improvement and Biotechnology, Jiangxi Normal University, Nanchang 330200, ChinaThe improvement of grain yield, quality, and resistance can be achieved through the utilization of heterosis. The combination of cytoplasmic male sterility (CMS) and fertility restoration (<i>Rf</i>) gene(s) greatly facilitates the commercial development of three-line hybrid rice based on heterosis. The basis for investigating the relationship between CMS and <i>Rf</i> genes lies in the rapid localization of wild rice fertility restoration genes. A set of the BC<sub>4</sub>F<sub>5</sub> population derived from interspecific crosses between Xieqingzao B (XB) and the BC<sub>1</sub>F<sub>9</sub> XB//Dongxiang wild rice (DWR)/XB line L5339 was used to detect quantitative trait loci (QTL) for fertility restoration. The population was then crossed with two male sterile lines, Zhong9A (Z9A) and DongB11A (DB11A), in order to generate a testcrossing population for investigating spikelet fertility. Based on the linkage mapping, seven QTLs were detected on chromosomes 1, 3, 5, 6, 8, and 10, explaining 2.76 to 12.46% of the phenotypic variation. Of them, two novel fertility restoration QTLs, <i>qRf3</i> and <i>qRf6</i>, can restore fertility of the CMS-DWR line DB11A by 16.56% and 15.12%, respectively. By employing joint QTL-seq and GradedPool-Seq methods, two novel <i>Rf</i> QTLs for DB11A, <i>qRf3</i> and <i>qRf6</i>, were identified at the physical locations of 10,900,001–11,700,000 bp and 28,016,785–31,247,556 bp, respectively. These findings are useful for exploring the natural variations of <i>Rf</i> genes in rice. Therefore, rice’s new genetic resources for the selection and breeding of rice restorer lines provide promising candidates for QTL fine localization and clarification.https://www.mdpi.com/1422-0067/24/19/14832fertility restorationDongxiang wild riceQTLGradedPool-Seq |
| spellingShingle | Wenshan Cai Wanlin Li Liuying Duan Yaling Chen Fantao Zhang Biaolin Hu Jiankun Xie Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis International Journal of Molecular Sciences fertility restoration Dongxiang wild rice QTL GradedPool-Seq |
| title | Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis |
| title_full | Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis |
| title_fullStr | Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis |
| title_full_unstemmed | Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis |
| title_short | Genetic Analysis of Novel Fertility Restoration Genes (<i>qRf3</i> and <i>qRf6</i>) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis |
| title_sort | genetic analysis of novel fertility restoration genes i qrf3 i and i qrf6 i in dongxiang wild rice using gradedpool seq mapping and qtl seq correlation analysis |
| topic | fertility restoration Dongxiang wild rice QTL GradedPool-Seq |
| url | https://www.mdpi.com/1422-0067/24/19/14832 |
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