A decline in avian cytokine expression with age revealed by commercially available cytokine array
Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially availabl...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1171782/full |
| _version_ | 1797830366556323840 |
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| author | Guy Levkovich Dana Almagor Ganesan Saibaba Inna Bendikov-Bar Mark Rusal Dmitri Lokshtanov Dmitri Shinder Dror Sagi |
| author_facet | Guy Levkovich Dana Almagor Ganesan Saibaba Inna Bendikov-Bar Mark Rusal Dmitri Lokshtanov Dmitri Shinder Dror Sagi |
| author_sort | Guy Levkovich |
| collection | DOAJ |
| description | Cytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially available cytokine array designed to assay circulating expression levels of multiple cytokines and immunomodulators simultaneously. Upon minor modifications to the manufacturer protocol, background noise was reduced, leading to a significant increase in the sensitivity of the device. Our data indicate that the array is reliable and produce consistent data between biological repeats. We tested the reproducibility of the array in a biologically relevant context by assessing age-related changes in circulating cytokines. While individual features did not show a consistent pattern, our data revealed a consistent decline in the median of all cytokine values, supporting the validity of the array in studying biological processes. |
| first_indexed | 2024-04-09T13:35:10Z |
| format | Article |
| id | doaj.art-859ee118344b480e988705ad3cf4f573 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-09T13:35:10Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-859ee118344b480e988705ad3cf4f5732023-05-09T11:52:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-04-011410.3389/fphys.2023.11717821171782A decline in avian cytokine expression with age revealed by commercially available cytokine arrayGuy Levkovich0Dana Almagor1Ganesan Saibaba2Inna Bendikov-Bar3Mark Rusal4Dmitri Lokshtanov5Dmitri Shinder6Dror Sagi7Agricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelKoret School of Veterinary Medicine, Hebrew University of Jerusalem, Jerusalem, IsraelAgricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelAgricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelAgricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelAgricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelAgricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelAgricultural Research Organization, Volcani Center, Institute of Animal Science, Rishon LeZion, IsraelCytokines are secreted immunomodulators that are key regulators of the avian immune response. Currently, the most commonly used method to follow cytokine expression is qPCR, which measures cellular levels of mRNA, rather their extracellular circulating levels. Here we present a commercially available cytokine array designed to assay circulating expression levels of multiple cytokines and immunomodulators simultaneously. Upon minor modifications to the manufacturer protocol, background noise was reduced, leading to a significant increase in the sensitivity of the device. Our data indicate that the array is reliable and produce consistent data between biological repeats. We tested the reproducibility of the array in a biologically relevant context by assessing age-related changes in circulating cytokines. While individual features did not show a consistent pattern, our data revealed a consistent decline in the median of all cytokine values, supporting the validity of the array in studying biological processes.https://www.frontiersin.org/articles/10.3389/fphys.2023.1171782/fullhensagingimmunologyarraycytokines |
| spellingShingle | Guy Levkovich Dana Almagor Ganesan Saibaba Inna Bendikov-Bar Mark Rusal Dmitri Lokshtanov Dmitri Shinder Dror Sagi A decline in avian cytokine expression with age revealed by commercially available cytokine array Frontiers in Physiology hens aging immunology array cytokines |
| title | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
| title_full | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
| title_fullStr | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
| title_full_unstemmed | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
| title_short | A decline in avian cytokine expression with age revealed by commercially available cytokine array |
| title_sort | decline in avian cytokine expression with age revealed by commercially available cytokine array |
| topic | hens aging immunology array cytokines |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1171782/full |
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