Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography
In this work, a series of experiments on the retention of B1 (riboflavin), B2 (thiamine) and B3 (nicotinic acid) vitamins in the HILIC and RPLC conditions have been performed involving the effects of organic modifier type and content, pH of the eluent and buffering salt (ammonium acetate) concentrat...
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MDPI AG
2022-07-01
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| Series: | AgriEngineering |
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| Online Access: | https://www.mdpi.com/2624-7402/4/3/38 |
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| author | Marcin Chutkowski Piotr Ziobrowski Mateusz Przywara Justyna Kamińska Wojciech Zapała |
| author_facet | Marcin Chutkowski Piotr Ziobrowski Mateusz Przywara Justyna Kamińska Wojciech Zapała |
| author_sort | Marcin Chutkowski |
| collection | DOAJ |
| description | In this work, a series of experiments on the retention of B1 (riboflavin), B2 (thiamine) and B3 (nicotinic acid) vitamins in the HILIC and RPLC conditions have been performed involving the effects of organic modifier type and content, pH of the eluent and buffering salt (ammonium acetate) concentration in the mobile phase as well as temperature of the system. For the needs of this study, three columns of different features have been chosen: Acclaim™ Mixed–Mode HILIC–1 (Dionex, Sunnyvale, CA, USA), Eurospher II 100–5 HILIC (Knauer, Berlin, Germany) and the RPLC column Nucleodur<sup>®</sup> C18 Gravity-SB (Macherey-Nagel, Duren, Germany). The influence of acetonitrile and methanol content in the eluent and process temperature have been tested and, based on that, the most promising systems have been selected regarding the possible separation of the vitamin mixture. Both the pH and buffering salt concentrations in the eluent have been adjusted in order to indicate the most effective system, which turned out to be the one involving the Nucleodur column and the eluent with 90% methanol, at pH 6 and <i>C<sub>buff</sub></i> = 20 mmol/dm<sup>3</sup>, which enables separation of the mixture within a time as short as 2.5 min at a 1.0 mL/s flowrate in isocratic conditions. |
| first_indexed | 2024-03-10T01:00:44Z |
| format | Article |
| id | doaj.art-85a23404970d4d04a25bbc9cd5ea9754 |
| institution | Directory Open Access Journal |
| issn | 2624-7402 |
| language | English |
| last_indexed | 2024-03-10T01:00:44Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | AgriEngineering |
| spelling | doaj.art-85a23404970d4d04a25bbc9cd5ea97542023-11-23T14:35:07ZengMDPI AGAgriEngineering2624-74022022-07-014356659110.3390/agriengineering4030038Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC ChromatographyMarcin Chutkowski0Piotr Ziobrowski1Mateusz Przywara2Justyna Kamińska3Wojciech Zapała4Department of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, PolandDepartment of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, PolandEuroservice Zakłady Przemysłu Tłuszczowego Surochów 160a, 37-500 Surochów, PolandDepartment of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, PolandIn this work, a series of experiments on the retention of B1 (riboflavin), B2 (thiamine) and B3 (nicotinic acid) vitamins in the HILIC and RPLC conditions have been performed involving the effects of organic modifier type and content, pH of the eluent and buffering salt (ammonium acetate) concentration in the mobile phase as well as temperature of the system. For the needs of this study, three columns of different features have been chosen: Acclaim™ Mixed–Mode HILIC–1 (Dionex, Sunnyvale, CA, USA), Eurospher II 100–5 HILIC (Knauer, Berlin, Germany) and the RPLC column Nucleodur<sup>®</sup> C18 Gravity-SB (Macherey-Nagel, Duren, Germany). The influence of acetonitrile and methanol content in the eluent and process temperature have been tested and, based on that, the most promising systems have been selected regarding the possible separation of the vitamin mixture. Both the pH and buffering salt concentrations in the eluent have been adjusted in order to indicate the most effective system, which turned out to be the one involving the Nucleodur column and the eluent with 90% methanol, at pH 6 and <i>C<sub>buff</sub></i> = 20 mmol/dm<sup>3</sup>, which enables separation of the mixture within a time as short as 2.5 min at a 1.0 mL/s flowrate in isocratic conditions.https://www.mdpi.com/2624-7402/4/3/38HILIC chromatographyRPLC chromatographyB1 vitaminB2 vitaminB3 vitamin |
| spellingShingle | Marcin Chutkowski Piotr Ziobrowski Mateusz Przywara Justyna Kamińska Wojciech Zapała Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography AgriEngineering HILIC chromatography RPLC chromatography B1 vitamin B2 vitamin B3 vitamin |
| title | Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography |
| title_full | Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography |
| title_fullStr | Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography |
| title_full_unstemmed | Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography |
| title_short | Studies on the Effects of Process Conditions on Separation of B1, B2 and B3 Vitamin Mixture Using HILIC and RPLC Chromatography |
| title_sort | studies on the effects of process conditions on separation of b1 b2 and b3 vitamin mixture using hilic and rplc chromatography |
| topic | HILIC chromatography RPLC chromatography B1 vitamin B2 vitamin B3 vitamin |
| url | https://www.mdpi.com/2624-7402/4/3/38 |
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