Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction
Objective: It is widely known that myocardial damage is not immediately terminated after the elimination of epicardial occlusion in cases of myocardial infarction. In situ thrombosis during epicardial occlusion might contribute to poor myocardial perfusion after reperfusion of an occluded epicardia...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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KARE Publishing
2016-02-01
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| Series: | Türk Kardiyoloji Derneği Arşivi |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tkd&un=TKDA-39345 |
| _version_ | 1797914362243973120 |
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| author | Emre Aslanger Seyhun Solakoğlu Öner Doğan Murat Sezer Sabahattin Umman |
| author_facet | Emre Aslanger Seyhun Solakoğlu Öner Doğan Murat Sezer Sabahattin Umman |
| author_sort | Emre Aslanger |
| collection | DOAJ |
| description | Objective: It is widely known that myocardial damage is not immediately terminated after the elimination of epicardial occlusion in cases of myocardial infarction. In situ thrombosis during epicardial occlusion might contribute to poor myocardial perfusion after reperfusion of an occluded epicardial artery. In the current study, we sought to determine the effects of ischemia and reperfusion on microvascular thrombotic occlusion.
Methods: Thirty male Wistar rats were included in the study. After the rats had been anesthetized and thoracotomized, the left coronary artery was occluded for 30 minutes in the first group, and it was occluded for 30 minutes and reperfused for an additional 20 minutes in the second group. Ten rats were used as a sham-operated control group. After completion of the study protocol, excised heart preparations were analyzed by immunohistochemistry and electron microscopy.
Results: A significant difference was found between the infarction plus reperfusion group and the other 2 groups, with respect to microvascular fibrin and thrombocyte deposition in immunohistochemistry analysis. These results were confirmed by morphological examination with electron microscopy.
Conclusion: In situ fibrin formation accompanies microvascular obstruction in acute myocardial infarction. Our results indicate that additional therapeutic approaches are needed in order to achieve better tissue perfusion in contemporary treatment of acute myocardial infarction after successful reopening of the infarct-related artery. |
| first_indexed | 2024-04-10T12:25:41Z |
| format | Article |
| id | doaj.art-85a2b7a2404e4dc49d35815ba31e8c7c |
| institution | Directory Open Access Journal |
| issn | 1016-5169 |
| language | English |
| last_indexed | 2024-04-10T12:25:41Z |
| publishDate | 2016-02-01 |
| publisher | KARE Publishing |
| record_format | Article |
| series | Türk Kardiyoloji Derneği Arşivi |
| spelling | doaj.art-85a2b7a2404e4dc49d35815ba31e8c7c2023-02-15T16:15:12ZengKARE PublishingTürk Kardiyoloji Derneği Arşivi1016-51692016-02-01441374410.5543/tkda.2015.39345TKDA-39345Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarctionEmre Aslanger0Seyhun Solakoğlu1Öner Doğan2Murat Sezer3Sabahattin Umman4Yeditepe University Hospital, Department of Cardiology, Istanbul, TurkeyIstanbul University, Istanbul Faculty of Medicine, Department of Histology And Embryology, İstanbul, TIstanbul University, Istanbul Faculty of Medicine, Department of Pathology, İstanbul, TurkeyYeditepe University Hospital, Department of Cardiology, Istanbul, TurkeyIstanbul University, Istanbul Faculty of Medicine, Department of Cardiology, İstanbul, TurkeyObjective: It is widely known that myocardial damage is not immediately terminated after the elimination of epicardial occlusion in cases of myocardial infarction. In situ thrombosis during epicardial occlusion might contribute to poor myocardial perfusion after reperfusion of an occluded epicardial artery. In the current study, we sought to determine the effects of ischemia and reperfusion on microvascular thrombotic occlusion. Methods: Thirty male Wistar rats were included in the study. After the rats had been anesthetized and thoracotomized, the left coronary artery was occluded for 30 minutes in the first group, and it was occluded for 30 minutes and reperfused for an additional 20 minutes in the second group. Ten rats were used as a sham-operated control group. After completion of the study protocol, excised heart preparations were analyzed by immunohistochemistry and electron microscopy. Results: A significant difference was found between the infarction plus reperfusion group and the other 2 groups, with respect to microvascular fibrin and thrombocyte deposition in immunohistochemistry analysis. These results were confirmed by morphological examination with electron microscopy. Conclusion: In situ fibrin formation accompanies microvascular obstruction in acute myocardial infarction. Our results indicate that additional therapeutic approaches are needed in order to achieve better tissue perfusion in contemporary treatment of acute myocardial infarction after successful reopening of the infarct-related artery.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tkd&un=TKDA-39345microvascular flowfibrin; myocardial infarction; no-reflow phenomenon; reperfusion injury. |
| spellingShingle | Emre Aslanger Seyhun Solakoğlu Öner Doğan Murat Sezer Sabahattin Umman Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction Türk Kardiyoloji Derneği Arşivi microvascular flow fibrin; myocardial infarction; no-reflow phenomenon; reperfusion injury. |
| title | Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction |
| title_full | Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction |
| title_fullStr | Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction |
| title_full_unstemmed | Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction |
| title_short | Microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction |
| title_sort | microvascular obstruction due to thrombosis and fibrin deposition in myocardial infarction |
| topic | microvascular flow fibrin; myocardial infarction; no-reflow phenomenon; reperfusion injury. |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tkd&un=TKDA-39345 |
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