Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties
Introduction: Probiotics are defined as live non-pathogenic microorganisms and if administered in adequate amounts, can have beneficial effects on their hosts. This study aimed to isolate Gram-positive bacteria from different sources and to evaluate their probiotic properties. Methods: We obtained f...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2017-01-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | http://jommid.pasteur.ac.ir/article-1-106-en.html |
| _version_ | 1818328868168138752 |
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| author | Muhammad Faisal Shahbaz Akram Muhammad Ashraf Sultan Ali Syeda Iqra Kazmi |
| author_facet | Muhammad Faisal Shahbaz Akram Muhammad Ashraf Sultan Ali Syeda Iqra Kazmi |
| author_sort | Muhammad Faisal Shahbaz Akram |
| collection | DOAJ |
| description | Introduction: Probiotics are defined as live non-pathogenic microorganisms and if administered in adequate amounts, can have beneficial effects on their hosts. This study aimed to isolate Gram-positive bacteria from different sources and to evaluate their probiotic properties. Methods: We obtained five samples from various sources including yogurt, infant feces, cheese, and soil under aseptic methods. The samples were inoculated on MRS agar and M17 medium. Biochemical assays identified isolated bacteria. The probiotic effect of isolated bacteria was tested by applying acidic pH 2.0, 0.3% w/v bile salt concentration and gastric juice in the samples. The antibiotic susceptibility profiling and their antimicrobial effects against Escherichia coli, Staphylococcus aureus, Salmonella enterica and Pseudomonas aeruginosa, were determined. Results: Four Gram-positive bacteria including Lactobacillus acidophilus, Bifidobacterium bifidum, Lactococcus lactis and Bacillus subtilis were isolated from yogurt, infant feces, cheese, and soil, respectively. Except for B. subtilis, all the isolated bacteria were catalase-negative and non-motile. The results showed that viable count of L. acidophilus, B. bifidum, and L. lactis was significantly high (P<0.05), and B. subtilis was sensitive. The isolates showed an antimicrobial effect against all indicator pathogens except P. aeruginosa. The isolated B. subtilis showed no antimicrobial effect. The antibiotic susceptibility was determined for ten different antibiotics. All the isolates were susceptible to most of the antibiotics (table 6) and resistant to penicillin G, gentamicin, vancomycin, and kanamycin. Conclusion: The species L. acidophilus, B. bifidum, and L. lactis fulfilled the criteria for probiotic properties and can be used in field conditions as probiotics. |
| first_indexed | 2024-12-13T12:39:00Z |
| format | Article |
| id | doaj.art-85a3b4f4d6c14ecca4557d588d65ccc4 |
| institution | Directory Open Access Journal |
| issn | 2345-5349 2345-5330 |
| language | English |
| last_indexed | 2024-12-13T12:39:00Z |
| publishDate | 2017-01-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj.art-85a3b4f4d6c14ecca4557d588d65ccc42022-12-21T23:45:42ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302017-01-01511216Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic PropertiesMuhammad Faisal Shahbaz Akram0Muhammad Ashraf1Sultan Ali2Syeda Iqra Kazmi3 Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan Introduction: Probiotics are defined as live non-pathogenic microorganisms and if administered in adequate amounts, can have beneficial effects on their hosts. This study aimed to isolate Gram-positive bacteria from different sources and to evaluate their probiotic properties. Methods: We obtained five samples from various sources including yogurt, infant feces, cheese, and soil under aseptic methods. The samples were inoculated on MRS agar and M17 medium. Biochemical assays identified isolated bacteria. The probiotic effect of isolated bacteria was tested by applying acidic pH 2.0, 0.3% w/v bile salt concentration and gastric juice in the samples. The antibiotic susceptibility profiling and their antimicrobial effects against Escherichia coli, Staphylococcus aureus, Salmonella enterica and Pseudomonas aeruginosa, were determined. Results: Four Gram-positive bacteria including Lactobacillus acidophilus, Bifidobacterium bifidum, Lactococcus lactis and Bacillus subtilis were isolated from yogurt, infant feces, cheese, and soil, respectively. Except for B. subtilis, all the isolated bacteria were catalase-negative and non-motile. The results showed that viable count of L. acidophilus, B. bifidum, and L. lactis was significantly high (P<0.05), and B. subtilis was sensitive. The isolates showed an antimicrobial effect against all indicator pathogens except P. aeruginosa. The isolated B. subtilis showed no antimicrobial effect. The antibiotic susceptibility was determined for ten different antibiotics. All the isolates were susceptible to most of the antibiotics (table 6) and resistant to penicillin G, gentamicin, vancomycin, and kanamycin. Conclusion: The species L. acidophilus, B. bifidum, and L. lactis fulfilled the criteria for probiotic properties and can be used in field conditions as probiotics.http://jommid.pasteur.ac.ir/article-1-106-en.htmlidentificationgram-positive bacteriaprobiotic |
| spellingShingle | Muhammad Faisal Shahbaz Akram Muhammad Ashraf Sultan Ali Syeda Iqra Kazmi Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties Journal of Medical Microbiology and Infectious Diseases identification gram-positive bacteria probiotic |
| title | Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties |
| title_full | Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties |
| title_fullStr | Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties |
| title_full_unstemmed | Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties |
| title_short | Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties |
| title_sort | isolation of gram positive bacteria from different sources and evaluation of their probiotic properties |
| topic | identification gram-positive bacteria probiotic |
| url | http://jommid.pasteur.ac.ir/article-1-106-en.html |
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