Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis
Acute lung injury occurs in 20–25% of cases following traumatic brain injury (TBI). We investigated changes in lung transcriptome expression post-TBI using animal models and bioinformatics. Employing unilateral controlled cortical impact for TBI, we conducted microarray analysis after lung acquisiti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/3018 |
| _version_ | 1797264370568265728 |
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| author | Wei-Hung Chan Shih-Ming Huang Yi-Lin Chiu |
| author_facet | Wei-Hung Chan Shih-Ming Huang Yi-Lin Chiu |
| author_sort | Wei-Hung Chan |
| collection | DOAJ |
| description | Acute lung injury occurs in 20–25% of cases following traumatic brain injury (TBI). We investigated changes in lung transcriptome expression post-TBI using animal models and bioinformatics. Employing unilateral controlled cortical impact for TBI, we conducted microarray analysis after lung acquisition, followed by gene set enrichment analysis of differentially expressed genes. Our findings indicate significant upregulation of inflammation-related genes and downregulation of nervous system genes. There was enhanced infiltration of adaptive immune cells, evidenced by positive enrichment in Lung-Th1, CD4, and CD8 T cells. Analysis using the Tabula Sapiens database revealed enrichment in lung-adventitial cells, pericytes, myofibroblasts, and fibroblasts, indicating potential effects on lung vasculature and fibrosis. Gene set enrichment analysis linked TBI to lung diseases, notably idiopathic pulmonary hypertension. A Venn diagram overlap analysis identified a common set of 20 genes, with <i>FOSL2</i> showing the most significant fold change. Additionally, we observed a significant increase in <i>ADRA1A</i>→<i>IL6</i> production post-TBI using the L1000 library. Our study highlights the impact of brain trauma on lung injury, revealing crucial gene expression changes related to immune cell infiltration, cytokine production, and potential alterations in lung vasculature and fibrosis, along with a specific spectrum of disease influence. |
| first_indexed | 2024-04-25T00:27:49Z |
| format | Article |
| id | doaj.art-85a49b2bc80c43f287b66d9cc5739deb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:27:49Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-85a49b2bc80c43f287b66d9cc5739deb2024-03-12T16:47:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255301810.3390/ijms25053018Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment AnalysisWei-Hung Chan0Shih-Ming Huang1Yi-Lin Chiu2Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Taipei City 114201, TaiwanDepartment of Biochemistry, National Defense Medical Center, Taipei City 114201, TaiwanDepartment of Biochemistry, National Defense Medical Center, Taipei City 114201, TaiwanAcute lung injury occurs in 20–25% of cases following traumatic brain injury (TBI). We investigated changes in lung transcriptome expression post-TBI using animal models and bioinformatics. Employing unilateral controlled cortical impact for TBI, we conducted microarray analysis after lung acquisition, followed by gene set enrichment analysis of differentially expressed genes. Our findings indicate significant upregulation of inflammation-related genes and downregulation of nervous system genes. There was enhanced infiltration of adaptive immune cells, evidenced by positive enrichment in Lung-Th1, CD4, and CD8 T cells. Analysis using the Tabula Sapiens database revealed enrichment in lung-adventitial cells, pericytes, myofibroblasts, and fibroblasts, indicating potential effects on lung vasculature and fibrosis. Gene set enrichment analysis linked TBI to lung diseases, notably idiopathic pulmonary hypertension. A Venn diagram overlap analysis identified a common set of 20 genes, with <i>FOSL2</i> showing the most significant fold change. Additionally, we observed a significant increase in <i>ADRA1A</i>→<i>IL6</i> production post-TBI using the L1000 library. Our study highlights the impact of brain trauma on lung injury, revealing crucial gene expression changes related to immune cell infiltration, cytokine production, and potential alterations in lung vasculature and fibrosis, along with a specific spectrum of disease influence.https://www.mdpi.com/1422-0067/25/5/3018traumatic brain injuryacute lung injuryimmune cell infiltrationbrain lung interaction |
| spellingShingle | Wei-Hung Chan Shih-Ming Huang Yi-Lin Chiu Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis International Journal of Molecular Sciences traumatic brain injury acute lung injury immune cell infiltration brain lung interaction |
| title | Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis |
| title_full | Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis |
| title_fullStr | Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis |
| title_full_unstemmed | Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis |
| title_short | Pulmonary Effects of Traumatic Brain Injury in Mice: A Gene Set Enrichment Analysis |
| title_sort | pulmonary effects of traumatic brain injury in mice a gene set enrichment analysis |
| topic | traumatic brain injury acute lung injury immune cell infiltration brain lung interaction |
| url | https://www.mdpi.com/1422-0067/25/5/3018 |
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