DEVELOPMENT OF THE ALGORITHM FOR IDENTIFICATION OF THE LEVEL OF VIBRIO CHOLERAE CTXA AND TOXR GENE EXPRESSION USING RT-PCR WITH REAL-TIME HYBRIDIZATION-FLUORESCENT REGISTRATION OF RESULTS

DEVELOPMENT OF THE ALGORITHM FOR IDENTIFICATION OF THE LEVEL OF VIBRIO CHOLERAE CTXA AND TOXR GENE EXPRESSION USING RT-PCR WITH REAL-TIME HYBRIDIZATION-FLUORESCENT REGISTRATION OF RESULTS

Objective of the study is to design the algorithm for assessment of expression of the structural and regulatory virulence Vibrio cholerae genes by the model of ctxA and toxR genes encoding and controlling biosynthesis of cholera toxin.Materials and methods. Utilized were 10 strains of Vibrio cholera...

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Bibliographic Details
Main Authors: A. A. Kritsky, N. B. Cheldyshova, I. V. Tuchkov, N. I. Smirnova
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2017-09-01
Series:Проблемы особо опасных инфекций
Subjects:
v. cholerae
cholera toxin
ctxa and toxr genes
rt-pcr
level of expression
Online Access:https://journal.microbe.ru/jour/article/view/412

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