Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract
Gold nanoparticles as one of the productions of the chemistry field have a special place in the cure of many diseases. Many experiences in medicinal researches have indicated that the plants enhance the anticancer effects of gold nanoparticles. According to the above contents, we investigated the ca...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535221005918 |
| _version_ | 1818286676185710592 |
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| author | Bing Zhu Na Xie Lulu Yue Kun Wang Mutasem Z. Bani-Fwaz Hosam-Eldin Hussein Osman Attalla F. El-kott Xuelian Bai |
| author_facet | Bing Zhu Na Xie Lulu Yue Kun Wang Mutasem Z. Bani-Fwaz Hosam-Eldin Hussein Osman Attalla F. El-kott Xuelian Bai |
| author_sort | Bing Zhu |
| collection | DOAJ |
| description | Gold nanoparticles as one of the productions of the chemistry field have a special place in the cure of many diseases. Many experiences in medicinal researches have indicated that the plants enhance the anticancer effects of gold nanoparticles. According to the above contents, we investigated the capacities of Spinacia oleracea L. leaf aqueous extract green-mediated gold nanoparticles (AuNPs) as a modern chemotherapeutic material in treating endometrial cancer. The physicochemical characterization tests including UV–Visible Spectroscopy (UV–Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and Transmission Electron Microscopy (TEM) were used for investigating the physicochemical properties of AuNPs. To survey the antioxidant potentials of AuNPs, one of the common antioxidant techniques i.e., DPPH was used. Determination of anti-endometrial cancer effects of AuNPs was carried out by the MTT assay and against Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines. The physicochemical characterization analyses revealed that the AuNPs had been formulated as the best possible. The results of the DPPH test confirmed excellent antioxidant properties of AuNPs in comparison to the butylated hydroxytoluene. The AuNPs IC50 was 194 µg/mL in the antioxidant test. The results of the MTT assay confirmed removing Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines after treating with low concentrations of AuNPs. The IC50 of the AuNPs was 341, 335, 316, and 325 µg/mL against Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines, respectively. The best finding of anti-endometrial cancer potentials was determined in the HEC-1-A cell line. According to the above results, significant anti-endometrial cancer effects of Au nanoparticles green-mediated by S. oleracea leaf extract are confirmed. It is offered that the studies of the clinical trial are performed for approving the above findings in humans. |
| first_indexed | 2024-12-13T01:28:22Z |
| format | Article |
| id | doaj.art-85a68fccfb96486f8eb5936c4846c04c |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-13T01:28:22Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-85a68fccfb96486f8eb5936c4846c04c2022-12-22T00:04:04ZengElsevierArabian Journal of Chemistry1878-53522022-03-01153103576Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extractBing Zhu0Na Xie1Lulu Yue2Kun Wang3Mutasem Z. Bani-Fwaz4Hosam-Eldin Hussein Osman5Attalla F. El-kott6Xuelian Bai7Department of gynaecology, The first people's hospital of Shangqiu, Shangqiu, Henan, 476100, ChinaDepartment of medical Hengshui People’s Hospital, Hengshui Hebei, 053000, ChinaDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital Of Nanchang University, Nanchang, Jiangxi, 330006, ChinaDepartment of Gastrointestinal, hepatobiliary and extrahepatic, The first people's hospital of Shangqiu, Shangqiu, Henan, 476100,ChinaDepartment of Chemistry, College of Science, King Khalid University, P. O. Box 9004, Abha 61413, Saudi ArabiaDepartment Anatomy, College of Medicine, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment Biology, College of Science, King Khalid University, Abha 61421, Saudi Arabia; Department Zoology, College of Science, Damanhour University, Damanhour 22511, EgyptDepartment of Oncology, Baotou Central Hospital, Baotou, inner Mongolia, 014040, China; Corresponding authors.Gold nanoparticles as one of the productions of the chemistry field have a special place in the cure of many diseases. Many experiences in medicinal researches have indicated that the plants enhance the anticancer effects of gold nanoparticles. According to the above contents, we investigated the capacities of Spinacia oleracea L. leaf aqueous extract green-mediated gold nanoparticles (AuNPs) as a modern chemotherapeutic material in treating endometrial cancer. The physicochemical characterization tests including UV–Visible Spectroscopy (UV–Vis), Field Emission Scanning Electron Microscopy (FE‐SEM), Fourier Transformed Infrared Spectroscopy (FT‐IR), and Transmission Electron Microscopy (TEM) were used for investigating the physicochemical properties of AuNPs. To survey the antioxidant potentials of AuNPs, one of the common antioxidant techniques i.e., DPPH was used. Determination of anti-endometrial cancer effects of AuNPs was carried out by the MTT assay and against Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines. The physicochemical characterization analyses revealed that the AuNPs had been formulated as the best possible. The results of the DPPH test confirmed excellent antioxidant properties of AuNPs in comparison to the butylated hydroxytoluene. The AuNPs IC50 was 194 µg/mL in the antioxidant test. The results of the MTT assay confirmed removing Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines after treating with low concentrations of AuNPs. The IC50 of the AuNPs was 341, 335, 316, and 325 µg/mL against Ishikawa, KLE, HEC-1-A, and HEC-1-B cell lines, respectively. The best finding of anti-endometrial cancer potentials was determined in the HEC-1-A cell line. According to the above results, significant anti-endometrial cancer effects of Au nanoparticles green-mediated by S. oleracea leaf extract are confirmed. It is offered that the studies of the clinical trial are performed for approving the above findings in humans.http://www.sciencedirect.com/science/article/pii/S1878535221005918Endometrial cancerGold nanoparticlesChemotherapeutic drugSpinacia oleracea L |
| spellingShingle | Bing Zhu Na Xie Lulu Yue Kun Wang Mutasem Z. Bani-Fwaz Hosam-Eldin Hussein Osman Attalla F. El-kott Xuelian Bai Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract Arabian Journal of Chemistry Endometrial cancer Gold nanoparticles Chemotherapeutic drug Spinacia oleracea L |
| title | Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract |
| title_full | Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract |
| title_fullStr | Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract |
| title_full_unstemmed | Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract |
| title_short | Formulation and characterization of a novel anti-human endometrial cancer supplement by gold nanoparticles green-synthesized using Spinacia oleracea L. Leaf aqueous extract |
| title_sort | formulation and characterization of a novel anti human endometrial cancer supplement by gold nanoparticles green synthesized using spinacia oleracea l leaf aqueous extract |
| topic | Endometrial cancer Gold nanoparticles Chemotherapeutic drug Spinacia oleracea L |
| url | http://www.sciencedirect.com/science/article/pii/S1878535221005918 |
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