Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft?
Abstract Background The purpose of this study was to use artery grafts filled with CACIPLIQ20 and see if they promote nerve regeneration. Methods Sixty male Wistar rats were used. The rats were randomly divided into four experimental groups (n = 15): transected control grou...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Georg Thieme Verlag KG
2019-01-01
|
| Series: | Journal of Reconstructive Microsurgery Open |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0039-1692453 |
| _version_ | 1818457857730805760 |
|---|---|
| author | George Alexopoulos Leonidas Pavlidis Myron Tsagarakis Alexandros Delimpaltas Antonios Tsimponis Apostolos Papalois Anna Batistatou Alexandra Papoudou-Bai Denis Barritault Efterpi Demiri Georgia Alexandra Spyropoulou |
| author_facet | George Alexopoulos Leonidas Pavlidis Myron Tsagarakis Alexandros Delimpaltas Antonios Tsimponis Apostolos Papalois Anna Batistatou Alexandra Papoudou-Bai Denis Barritault Efterpi Demiri Georgia Alexandra Spyropoulou |
| author_sort | George Alexopoulos |
| collection | DOAJ |
| description | Abstract
Background The purpose of this study was to use artery grafts filled with CACIPLIQ20 and see if they promote nerve regeneration.
Methods Sixty male Wistar rats were used. The rats were randomly divided into four experimental groups (n = 15): transected control group (negative control group [NCG]), sham-operated group (positive control group [SO]) artery graft group filled with saline (AG/NS), and CACIPLIQ20-treated group (AG/CACIPLIQ20). Fifteen rats were used as artery graft donors. In the SO group, the sciatic nerve was dissected from the surrounding tissues and left intact. In the NCG, AG/NS and AG/CACIPLIQ20) groups, a 10-mm gap was created in the left sciatic nerve. In the NCG group, the gap was not bridged with a graft. In the AG/NS group, the gap was bridged with a graft filled with saline. In the AG/CACIPLIQ20 group, the graft was filled with CACIPLIQ20. Walking track analysis was performed at 4, 8, 12, and 16 weeks after surgery. At 16 weeks postoperatively, the rats were sacrificed, nerve sections were harvested for histopathology analysis, and the weight ratio of the gastrocnemius muscle was measured.
Results There was no significant difference in myelin sheath thickness between the AG/NS and AG/CACIPLIQ20 groups. Muscle weight in the AG/CACIPLIQ20 group was higher but not statistically significant (p = 0.168) compared with the AG/NS group. Also, AG/CACIPLIQ20 mean was better than AG/NS mean, although there was no statistically significant difference (p = 0.605).
Conclusion There could be an indication that CACIPLIQ20 improves functional recovery of a transected peripheral nerve through a nerve gap bridged with an artery graft. |
| first_indexed | 2024-12-14T22:49:14Z |
| format | Article |
| id | doaj.art-85a6e7167d3e40b68f2a41f2ab12b378 |
| institution | Directory Open Access Journal |
| issn | 2377-0813 2377-0821 |
| language | English |
| last_indexed | 2024-12-14T22:49:14Z |
| publishDate | 2019-01-01 |
| publisher | Georg Thieme Verlag KG |
| record_format | Article |
| series | Journal of Reconstructive Microsurgery Open |
| spelling | doaj.art-85a6e7167d3e40b68f2a41f2ab12b3782022-12-21T22:44:47ZengGeorg Thieme Verlag KGJournal of Reconstructive Microsurgery Open2377-08132377-08212019-01-010401e47e5310.1055/s-0039-1692453Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft?George Alexopoulos0Leonidas Pavlidis1Myron Tsagarakis2Alexandros Delimpaltas3Antonios Tsimponis4Apostolos Papalois5Anna Batistatou6Alexandra Papoudou-Bai7Denis Barritault8Efterpi Demiri9Georgia Alexandra Spyropoulou10Clinic of Plastic Surgery, Thriasio General Hospital, Magoula GreeceClinic of Plastic Surgery, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceDepartment of Plastic and Reconstructive Surgery, ENI Medical Group, Kavos Lefkimi, Corfu, GreeceClinic of Plastic Surgery, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceClinic of Plastic Surgery, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceDepartment of Research and Development, "ELPEN" Pharmaceuticals, Athens, GreeceDepartment of Pathology, Panepistimio Ioanninon, Ioannina, GreeceDepartment of Pathology, Panepistimio Ioanninon, Ioannina, GreeceOTR3 Company, Paris, FranceClinic of Plastic Surgery, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceClinic of Plastic Surgery, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceAbstract Background The purpose of this study was to use artery grafts filled with CACIPLIQ20 and see if they promote nerve regeneration. Methods Sixty male Wistar rats were used. The rats were randomly divided into four experimental groups (n = 15): transected control group (negative control group [NCG]), sham-operated group (positive control group [SO]) artery graft group filled with saline (AG/NS), and CACIPLIQ20-treated group (AG/CACIPLIQ20). Fifteen rats were used as artery graft donors. In the SO group, the sciatic nerve was dissected from the surrounding tissues and left intact. In the NCG, AG/NS and AG/CACIPLIQ20) groups, a 10-mm gap was created in the left sciatic nerve. In the NCG group, the gap was not bridged with a graft. In the AG/NS group, the gap was bridged with a graft filled with saline. In the AG/CACIPLIQ20 group, the graft was filled with CACIPLIQ20. Walking track analysis was performed at 4, 8, 12, and 16 weeks after surgery. At 16 weeks postoperatively, the rats were sacrificed, nerve sections were harvested for histopathology analysis, and the weight ratio of the gastrocnemius muscle was measured. Results There was no significant difference in myelin sheath thickness between the AG/NS and AG/CACIPLIQ20 groups. Muscle weight in the AG/CACIPLIQ20 group was higher but not statistically significant (p = 0.168) compared with the AG/NS group. Also, AG/CACIPLIQ20 mean was better than AG/NS mean, although there was no statistically significant difference (p = 0.605). Conclusion There could be an indication that CACIPLIQ20 improves functional recovery of a transected peripheral nerve through a nerve gap bridged with an artery graft.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0039-1692453peripheral nerve regenerationnerve gapheparin-binding growth factors |
| spellingShingle | George Alexopoulos Leonidas Pavlidis Myron Tsagarakis Alexandros Delimpaltas Antonios Tsimponis Apostolos Papalois Anna Batistatou Alexandra Papoudou-Bai Denis Barritault Efterpi Demiri Georgia Alexandra Spyropoulou Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft? Journal of Reconstructive Microsurgery Open peripheral nerve regeneration nerve gap heparin-binding growth factors |
| title | Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft? |
| title_full | Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft? |
| title_fullStr | Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft? |
| title_full_unstemmed | Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft? |
| title_short | Can ReGeneraTing Agents Improve Functional Recovery of Transected Peripheral Nerve through a Nerve Gap Bridged with an Artery Graft? |
| title_sort | can regenerating agents improve functional recovery of transected peripheral nerve through a nerve gap bridged with an artery graft |
| topic | peripheral nerve regeneration nerve gap heparin-binding growth factors |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0039-1692453 |
| work_keys_str_mv | AT georgealexopoulos canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT leonidaspavlidis canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT myrontsagarakis canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT alexandrosdelimpaltas canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT antoniostsimponis canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT apostolospapalois canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT annabatistatou canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT alexandrapapoudoubai canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT denisbarritault canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT efterpidemiri canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft AT georgiaalexandraspyropoulou canregeneratingagentsimprovefunctionalrecoveryoftransectedperipheralnervethroughanervegapbridgedwithanarterygraft |