Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis
Abstract Background DNA damage repair (DDR) plays a role in the tumorigenesis and progression of lung squamous cell carcinoma (LUSC), but the predictive value of DDR in LUSC has not been fully elucidated. Methods The LUSC datasets were retrieved from the Cancer Genome Atlas databases. Univariate Cox...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-04-01
|
| Series: | Thoracic Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1759-7714.14370 |
| _version_ | 1817990464639336448 |
|---|---|
| author | Bin Jia Ting Gong Bingsheng Sun Zhenfa Zhang Diansheng Zhong Changli Wang |
| author_facet | Bin Jia Ting Gong Bingsheng Sun Zhenfa Zhang Diansheng Zhong Changli Wang |
| author_sort | Bin Jia |
| collection | DOAJ |
| description | Abstract Background DNA damage repair (DDR) plays a role in the tumorigenesis and progression of lung squamous cell carcinoma (LUSC), but the predictive value of DDR in LUSC has not been fully elucidated. Methods The LUSC datasets were retrieved from the Cancer Genome Atlas databases. Univariate Cox regression and least absolute shrinkage and selection operator regression were integrated to identify critical genes and construct a DDR gene signature. We performed Kaplan–Meier (KM) curve to compare the overall survival (OS) between the two groups based on DDR signature and used the CIBERSORT tool to compare the immune cell composition. Further gene set enrichment analysis (GSEA) was performed on the differential expressed genes. Result We established the DDR‐related gene signature on LUSC. KM curve showed the low‐risk group had a better prognosis than the high‐risk group in the training set (p = 0.022673) and the complete set (p = 0.003201). The area under receiver operating characteristic curve for OS was 0.98, 0.96, and 0.97 in the training dataset, testing dataset, and the complete dataset, respectively. The composition of immune cells was different between the high‐ and low‐risk group. The GSEA result suggests that genes of the patients in low‐risk group were mainly enriched in the DNA adducts; drug metabolism‐cytochrome P450, metabolism of xenobiotics by cytochrome P450. Conclusion This study identified DDR‐associated potential biomarkers related to overall survival of LUSC and establishes the DDR‐associated gene signature. |
| first_indexed | 2024-04-14T00:59:22Z |
| format | Article |
| id | doaj.art-85a7fcc72c154668959227e00e48439b |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-04-14T00:59:22Z |
| publishDate | 2022-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-85a7fcc72c154668959227e00e48439b2022-12-22T02:21:28ZengWileyThoracic Cancer1759-77061759-77142022-04-011381143115210.1111/1759-7714.14370Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosisBin Jia0Ting Gong1Bingsheng Sun2Zhenfa Zhang3Diansheng Zhong4Changli Wang5Department of Lung Cancer, Tianjin Lung Cancer Center Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer Tianjin ChinaDepartment of Medical Oncology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Lung Cancer, Tianjin Lung Cancer Center Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer Tianjin ChinaDepartment of Lung Cancer, Tianjin Lung Cancer Center Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer Tianjin ChinaDepartment of Medical Oncology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Lung Cancer, Tianjin Lung Cancer Center Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer Tianjin ChinaAbstract Background DNA damage repair (DDR) plays a role in the tumorigenesis and progression of lung squamous cell carcinoma (LUSC), but the predictive value of DDR in LUSC has not been fully elucidated. Methods The LUSC datasets were retrieved from the Cancer Genome Atlas databases. Univariate Cox regression and least absolute shrinkage and selection operator regression were integrated to identify critical genes and construct a DDR gene signature. We performed Kaplan–Meier (KM) curve to compare the overall survival (OS) between the two groups based on DDR signature and used the CIBERSORT tool to compare the immune cell composition. Further gene set enrichment analysis (GSEA) was performed on the differential expressed genes. Result We established the DDR‐related gene signature on LUSC. KM curve showed the low‐risk group had a better prognosis than the high‐risk group in the training set (p = 0.022673) and the complete set (p = 0.003201). The area under receiver operating characteristic curve for OS was 0.98, 0.96, and 0.97 in the training dataset, testing dataset, and the complete dataset, respectively. The composition of immune cells was different between the high‐ and low‐risk group. The GSEA result suggests that genes of the patients in low‐risk group were mainly enriched in the DNA adducts; drug metabolism‐cytochrome P450, metabolism of xenobiotics by cytochrome P450. Conclusion This study identified DDR‐associated potential biomarkers related to overall survival of LUSC and establishes the DDR‐associated gene signature.https://doi.org/10.1111/1759-7714.14370DNA repairlung squamous cell carcinomaprognosissignature |
| spellingShingle | Bin Jia Ting Gong Bingsheng Sun Zhenfa Zhang Diansheng Zhong Changli Wang Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis Thoracic Cancer DNA repair lung squamous cell carcinoma prognosis signature |
| title | Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis |
| title_full | Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis |
| title_fullStr | Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis |
| title_full_unstemmed | Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis |
| title_short | Identification of a DNA damage repair gene‐related signature for lung squamous cell carcinoma prognosis |
| title_sort | identification of a dna damage repair gene related signature for lung squamous cell carcinoma prognosis |
| topic | DNA repair lung squamous cell carcinoma prognosis signature |
| url | https://doi.org/10.1111/1759-7714.14370 |
| work_keys_str_mv | AT binjia identificationofadnadamagerepairgenerelatedsignatureforlungsquamouscellcarcinomaprognosis AT tinggong identificationofadnadamagerepairgenerelatedsignatureforlungsquamouscellcarcinomaprognosis AT bingshengsun identificationofadnadamagerepairgenerelatedsignatureforlungsquamouscellcarcinomaprognosis AT zhenfazhang identificationofadnadamagerepairgenerelatedsignatureforlungsquamouscellcarcinomaprognosis AT dianshengzhong identificationofadnadamagerepairgenerelatedsignatureforlungsquamouscellcarcinomaprognosis AT changliwang identificationofadnadamagerepairgenerelatedsignatureforlungsquamouscellcarcinomaprognosis |