Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism
Postbiotics are rich in a variety of bioactive components, which may have beneficial effects in inhibiting hepatic lipid accumulation. In this study, we investigated the preventive effects of postbiotics (POST) prepared from <i>Lactobacillus paracasei</i> on non-alcoholic fatty liver dis...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13522 |
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| author | Zhenghao Pan Bingyong Mao Qiuxiang Zhang Xin Tang Bo Yang Jianxin Zhao Shumao Cui Hao Zhang |
| author_facet | Zhenghao Pan Bingyong Mao Qiuxiang Zhang Xin Tang Bo Yang Jianxin Zhao Shumao Cui Hao Zhang |
| author_sort | Zhenghao Pan |
| collection | DOAJ |
| description | Postbiotics are rich in a variety of bioactive components, which may have beneficial effects in inhibiting hepatic lipid accumulation. In this study, we investigated the preventive effects of postbiotics (POST) prepared from <i>Lactobacillus paracasei</i> on non-alcoholic fatty liver disease (NAFLD). Our results showed that when mice ingested a high-fat diet (HFD) and POST simultaneously, weight gain was slowed, epididymal white fat hypertrophy and insulin resistance were suppressed, serum biochemical indicators related to blood lipid metabolism were improved, and hepatic steatosis and liver inflammation decreased. Bacterial sequencing showed that POST modulated the gut microbiota in HFD mice, increasing the relative abundance of <i>Akkermansia</i> and reducing the relative abundance of <i>Lachnospiraceae NK4A136 group</i>, <i>Ruminiclostridium</i> and <i>Bilophila</i>. Spearman’s correlation analysis revealed significant correlations between lipid metabolism parameters and gut microbes. Functional prediction results showed that the regulation of gut microbiota was associated with the improvement of metabolic status. The metabolomic analysis of the liver revealed that POST-regulated liver metabolic pathways, such as glycerophospholipid and ether lipid metabolism, pantothenate and CoA biosynthesis, some parts of amino acid metabolism, and other metabolic pathways. In addition, POST regulated the gene expression in hepatocytes at the mRNA level, thereby regulating lipid metabolism. These findings suggest that POST plays a protective role against NAFLD and may exert its efficacy by modulating the gut microbiota and liver metabolism, and these findings may be applied to related functional foods. |
| first_indexed | 2024-03-09T18:59:43Z |
| format | Article |
| id | doaj.art-85a9b8a393e34ff2ad07185bce37ef08 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:59:43Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-85a9b8a393e34ff2ad07185bce37ef082023-11-24T05:09:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211352210.3390/ijms232113522Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver MetabolismZhenghao Pan0Bingyong Mao1Qiuxiang Zhang2Xin Tang3Bo Yang4Jianxin Zhao5Shumao Cui6Hao Zhang7State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaPostbiotics are rich in a variety of bioactive components, which may have beneficial effects in inhibiting hepatic lipid accumulation. In this study, we investigated the preventive effects of postbiotics (POST) prepared from <i>Lactobacillus paracasei</i> on non-alcoholic fatty liver disease (NAFLD). Our results showed that when mice ingested a high-fat diet (HFD) and POST simultaneously, weight gain was slowed, epididymal white fat hypertrophy and insulin resistance were suppressed, serum biochemical indicators related to blood lipid metabolism were improved, and hepatic steatosis and liver inflammation decreased. Bacterial sequencing showed that POST modulated the gut microbiota in HFD mice, increasing the relative abundance of <i>Akkermansia</i> and reducing the relative abundance of <i>Lachnospiraceae NK4A136 group</i>, <i>Ruminiclostridium</i> and <i>Bilophila</i>. Spearman’s correlation analysis revealed significant correlations between lipid metabolism parameters and gut microbes. Functional prediction results showed that the regulation of gut microbiota was associated with the improvement of metabolic status. The metabolomic analysis of the liver revealed that POST-regulated liver metabolic pathways, such as glycerophospholipid and ether lipid metabolism, pantothenate and CoA biosynthesis, some parts of amino acid metabolism, and other metabolic pathways. In addition, POST regulated the gene expression in hepatocytes at the mRNA level, thereby regulating lipid metabolism. These findings suggest that POST plays a protective role against NAFLD and may exert its efficacy by modulating the gut microbiota and liver metabolism, and these findings may be applied to related functional foods.https://www.mdpi.com/1422-0067/23/21/13522postbioticshigh-fat dietNAFLDgut microbiotaliver metabolome |
| spellingShingle | Zhenghao Pan Bingyong Mao Qiuxiang Zhang Xin Tang Bo Yang Jianxin Zhao Shumao Cui Hao Zhang Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism International Journal of Molecular Sciences postbiotics high-fat diet NAFLD gut microbiota liver metabolome |
| title | Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism |
| title_full | Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism |
| title_fullStr | Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism |
| title_full_unstemmed | Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism |
| title_short | Postbiotics Prepared Using <i>Lactobacillus paracasei</i> CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism |
| title_sort | postbiotics prepared using i lactobacillus paracasei i ccfm1224 prevent nonalcoholic fatty liver disease by modulating the gut microbiota and liver metabolism |
| topic | postbiotics high-fat diet NAFLD gut microbiota liver metabolome |
| url | https://www.mdpi.com/1422-0067/23/21/13522 |
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