The Prevalence of Human Papillomavirus Genotypes in Women with Abnormal Cervical Cytology
Introduction: Human papillomavirus (HPV) is the major cause of cervical cancer, which is the second most common cancer in the world among women and is one of the major causes of cancer-related deaths in developing countries. Approximately 500.000 new cervical cancers are identified each year in the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2018-12-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2018-23-04-186-192.pdf |
| Summary: | Introduction: Human papillomavirus (HPV) is the major cause of cervical cancer, which is the second most common cancer in the world among women and is one of the major causes of cancer-related deaths in developing countries. Approximately 500.000 new cervical cancers are identified each year in the world, and close to half of them are estimated to have resulted in death. Cervical cancer is largely a preventable disease by early detection and treatment of cervical precancerous lesions with the combined use of high risk (HR) HPV DNA testing and cervical cytology test in cervical cancer screening. Prophylactic human papillomavirus vaccines, which provide primary protection against cervical cancer, have been started to be applied since 2006 with the aim of decreasing the incidence of cervical cancer and mortality. HPV vaccine has been proven to be effective in protecting against precancerous lesions by providing immunity against high risk (HR) HPV types and has been included in the routine vaccination program in many countries. The aim of this study was to investigate the prevalence and genotypes of HPV using real-time PCR test in women with abnormal cervical cytology. Materials and Methods: Between 2015-2017, a total of 315 women aged between 30 and 65 years were screened for HPV in the Clinic of Gynecology and Obstetrics, Faculty of Medicine, Cukurova University. Out of the 315 female cervical swabs, a total of 53 had abnormal cervical cytology and 262 had normal cervical cytology. HPV genotyping from cervical swab samples was performed by realtime PCR using Bosphore HPV Genotyping High Risk Kit v1 (Anatolia Geneworks, Turkey). Results: The prevalence of HPV infection in 53 women with abnormal cytology was 69.8% (n= 37) and 39.7% (n= 104) in the control group. The most prevalent HPV genotype among 37 HPV-positive women with abnormal cytology was HPV 16 (45.9%), followed by HPV 56 (18.9%), HPV 18 (16.2%) HPV 52 (13.5%), HPV 68 (13.5%), HPV33 (10.8%), HPV35 (10.8%), HPV 58 (10.8%), HPV 51(8.1%), HPV 39 (5.4%), HPV 45 (5.4%), and HPV 66 (5.4%). Among HPV-positive 104 women as the control group, the most common genotypes were HPV 16 (52.9%), HPV 18 (27.9%), HPV 45 (13.5%), HPV 56 (13.5%), HPV 68 (12.5%,) and HPV 52 (8.7%). Conclusion: The most common genotype was HPV 16 in women with cervical precancerous lesions, and the diagnosis of oncogenic HPV genotypes is necessary to identify women at risk for cervical cancer. |
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| ISSN: | 1300-932X 1300-932X |