Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products
<i>O</i>-methylation of flavonoids is an important modification reaction that occurs in plants. <i>O</i>-methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, an <i>O</i>-meth...
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MDPI AG
2020-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/4/858 |
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| author | Xiaojuan Liu Yue Wang Yezhi Chen Shuting Xu Qin Gong Chenning Zhao Jinping Cao Chongde Sun |
| author_facet | Xiaojuan Liu Yue Wang Yezhi Chen Shuting Xu Qin Gong Chenning Zhao Jinping Cao Chongde Sun |
| author_sort | Xiaojuan Liu |
| collection | DOAJ |
| description | <i>O</i>-methylation of flavonoids is an important modification reaction that occurs in plants. <i>O</i>-methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, an <i>O</i>-methyltransferase gene (<i>CrOMT2</i>) was isolated from the fruit peel of <i>Citrus reticulata</i>, which encoding a multifunctional <i>O</i>-methyltransferase and could effectively catalyze the methylation of 3’-, 5’-, and 7-OH of flavonoids with vicinal hydroxyl substitutions. Substrate preference assays indicated that this recombinant enzyme favored polymethoxylated flavones (PMF)-type substrates in vitro, thereby providing biochemical evidence for the potential role of the enzyme in plants. Additionally, the cytotoxicity of the methylated products from the enzymatic catalytic reaction was evaluated in vitro using human gastric cell lines SGC-7901 and BGC-823. The results showed that the in vitro cytotoxicity of the flavonoids with the unsaturated C2-C3 bond was increased after being methylated at position 3’. These combined results provide biochemical insight regarding CrOMT2 in vitro and indicate the in vitro cytotoxicity of the products methylated by its catalytic reaction. |
| first_indexed | 2024-04-14T00:14:20Z |
| format | Article |
| id | doaj.art-85b01a6e10a04d7799b9697c48f31602 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-14T00:14:20Z |
| publishDate | 2020-02-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-85b01a6e10a04d7799b9697c48f316022022-12-22T02:23:11ZengMDPI AGMolecules1420-30492020-02-0125485810.3390/molecules25040858molecules25040858Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated ProductsXiaojuan Liu0Yue Wang1Yezhi Chen2Shuting Xu3Qin Gong4Chenning Zhao5Jinping Cao6Chongde Sun7College of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China<i>O</i>-methylation of flavonoids is an important modification reaction that occurs in plants. <i>O</i>-methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, an <i>O</i>-methyltransferase gene (<i>CrOMT2</i>) was isolated from the fruit peel of <i>Citrus reticulata</i>, which encoding a multifunctional <i>O</i>-methyltransferase and could effectively catalyze the methylation of 3’-, 5’-, and 7-OH of flavonoids with vicinal hydroxyl substitutions. Substrate preference assays indicated that this recombinant enzyme favored polymethoxylated flavones (PMF)-type substrates in vitro, thereby providing biochemical evidence for the potential role of the enzyme in plants. Additionally, the cytotoxicity of the methylated products from the enzymatic catalytic reaction was evaluated in vitro using human gastric cell lines SGC-7901 and BGC-823. The results showed that the in vitro cytotoxicity of the flavonoids with the unsaturated C2-C3 bond was increased after being methylated at position 3’. These combined results provide biochemical insight regarding CrOMT2 in vitro and indicate the in vitro cytotoxicity of the products methylated by its catalytic reaction.https://www.mdpi.com/1420-3049/25/4/858citrusflavonoids<i>o</i>-methyltransferasebioactivitycytotoxicity |
| spellingShingle | Xiaojuan Liu Yue Wang Yezhi Chen Shuting Xu Qin Gong Chenning Zhao Jinping Cao Chongde Sun Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products Molecules citrus flavonoids <i>o</i>-methyltransferase bioactivity cytotoxicity |
| title | Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products |
| title_full | Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products |
| title_fullStr | Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products |
| title_full_unstemmed | Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products |
| title_short | Characterization of a Flavonoid 3’/5’/7-<i>O</i>-Methyltransferase from <i>Citrus reticulata</i> and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products |
| title_sort | characterization of a flavonoid 3 5 7 i o i methyltransferase from i citrus reticulata i and evaluation of the in vitro cytotoxicity of its methylated products |
| topic | citrus flavonoids <i>o</i>-methyltransferase bioactivity cytotoxicity |
| url | https://www.mdpi.com/1420-3049/25/4/858 |
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