Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment
Abstract Background The combination of low level laser therapy (LLLT) and nano-sized particles (NPs) of chitosan (Chit) is hypothesized to be more effective in skin wound healing. A total of 63 CD1 mice were divided into the normal group and injured mice groups that were subjected to a 21-day therap...
| Những tác giả chính: | , , , , |
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| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
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SpringerOpen
2025-03-01
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| Loạt: | Journal of Basic and Applied Zoology |
| Những chủ đề: | |
| Truy cập trực tuyến: | https://doi.org/10.1186/s41936-025-00433-w |
| _version_ | 1826538120206614528 |
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| author | Nehal Mohamed Osama Fekry Ahmed Al Balah Mohamed Refat Abeer Mahmoud Badr Ahmed Afifi |
| author_facet | Nehal Mohamed Osama Fekry Ahmed Al Balah Mohamed Refat Abeer Mahmoud Badr Ahmed Afifi |
| author_sort | Nehal Mohamed |
| collection | DOAJ |
| description | Abstract Background The combination of low level laser therapy (LLLT) and nano-sized particles (NPs) of chitosan (Chit) is hypothesized to be more effective in skin wound healing. A total of 63 CD1 mice were divided into the normal group and injured mice groups that were subjected to a 21-day therapy course of laser exposure (650 ± 5 nm, power density 180 mw/cm2), topically applied 2% Chit NPs and a combination of both. Results On day 21, the wound healing rate reached 100% in mice treated by laser plus Chit NPs while other single treatments did not reach this record. After 48 h, therapy with laser plus Chit NPs significantly enhanced re-epithelization, granulation, inflammation, and angiogenesis scores compared to the non-treated wound group. After 21 days, the other single treatments showed improvements to some histological parameters, but the combined treatment exhibited complete epidermal remodeling with a decrease in inflammatory exudate. During the late phase, the count of RBCs and hemoglobin% was significantly increased in the treated group of laser plus Chit NPs. In contrast, the differential count of neutrophils, lymphocytes, and monocytes was markedly lower than in the untreated wound group. Within 48 h, the group that received laser plus Chit NPs treatment showed a significant (P < 0.001) decrease in the elevated serum levels of TNF-α and IL-1β compared to the non-treated wound group. Conclusions Therapy by laser or Chit NPs alone showed beneficial effects, but a combination of both accelerated the wound healing rate and displayed the highest improved lesion score. |
| first_indexed | 2025-03-14T03:21:33Z |
| format | Article |
| id | doaj.art-85b53a80c197458ea86480b8b72dd4d1 |
| institution | Directory Open Access Journal |
| issn | 2090-990X |
| language | English |
| last_indexed | 2025-03-14T03:21:33Z |
| publishDate | 2025-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj.art-85b53a80c197458ea86480b8b72dd4d12025-03-09T12:35:19ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2025-03-0186111010.1186/s41936-025-00433-wLow-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessmentNehal Mohamed0Osama Fekry Ahmed Al Balah1Mohamed Refat2Abeer Mahmoud Badr3Ahmed Afifi4Zoology Department, Faculty of Science, Cairo UniversityNational Institute of Laser Enhanced Sciences, Cairo UniversityPathology Department, Faculty of Veterinary Medicine, Cairo UniversityZoology Department, Faculty of Science, Cairo UniversityZoology Department, Faculty of Science, Cairo UniversityAbstract Background The combination of low level laser therapy (LLLT) and nano-sized particles (NPs) of chitosan (Chit) is hypothesized to be more effective in skin wound healing. A total of 63 CD1 mice were divided into the normal group and injured mice groups that were subjected to a 21-day therapy course of laser exposure (650 ± 5 nm, power density 180 mw/cm2), topically applied 2% Chit NPs and a combination of both. Results On day 21, the wound healing rate reached 100% in mice treated by laser plus Chit NPs while other single treatments did not reach this record. After 48 h, therapy with laser plus Chit NPs significantly enhanced re-epithelization, granulation, inflammation, and angiogenesis scores compared to the non-treated wound group. After 21 days, the other single treatments showed improvements to some histological parameters, but the combined treatment exhibited complete epidermal remodeling with a decrease in inflammatory exudate. During the late phase, the count of RBCs and hemoglobin% was significantly increased in the treated group of laser plus Chit NPs. In contrast, the differential count of neutrophils, lymphocytes, and monocytes was markedly lower than in the untreated wound group. Within 48 h, the group that received laser plus Chit NPs treatment showed a significant (P < 0.001) decrease in the elevated serum levels of TNF-α and IL-1β compared to the non-treated wound group. Conclusions Therapy by laser or Chit NPs alone showed beneficial effects, but a combination of both accelerated the wound healing rate and displayed the highest improved lesion score.https://doi.org/10.1186/s41936-025-00433-wSkin wound healingLLLTChitosan NPsCytokinesHealing rateHistology |
| spellingShingle | Nehal Mohamed Osama Fekry Ahmed Al Balah Mohamed Refat Abeer Mahmoud Badr Ahmed Afifi Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment Journal of Basic and Applied Zoology Skin wound healing LLLT Chitosan NPs Cytokines Healing rate Histology |
| title | Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment |
| title_full | Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment |
| title_fullStr | Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment |
| title_full_unstemmed | Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment |
| title_short | Low-level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice: histological, hematological, and proinflammatory cytokines assessment |
| title_sort | low level laser and chitosan nanoparticles therapy speeds up the process of skin wound healing in mice histological hematological and proinflammatory cytokines assessment |
| topic | Skin wound healing LLLT Chitosan NPs Cytokines Healing rate Histology |
| url | https://doi.org/10.1186/s41936-025-00433-w |
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