Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization
AIM: To explore the synthesis of thermo-sensitive poly N-isopropylacry-lamide(PNIPAAm)and the petri dish grafted with PNIPAAm hydrogels by the electron accelerator, as well as the growth conditions and the biological characteristics of rabbit corneal stromal cells on thermo-sensitive PNIPAAm hydroge...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2024-05-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2024/5/202405005.pdf |
| _version_ | 1797194671233957888 |
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| author | Wang Wei Zhang Pengfei Chen Chunxia Qian Li Chen Jiansu |
| author_facet | Wang Wei Zhang Pengfei Chen Chunxia Qian Li Chen Jiansu |
| author_sort | Wang Wei |
| collection | DOAJ |
| description | AIM: To explore the synthesis of thermo-sensitive poly N-isopropylacry-lamide(PNIPAAm)and the petri dish grafted with PNIPAAm hydrogels by the electron accelerator, as well as the growth conditions and the biological characteristics of rabbit corneal stromal cells on thermo-sensitive PNIPAAm hydrogels, and the cell sheets obtained from the PNIPAAm hydrogels.METHODS: NIPAAm monomer was dissolved in 2-propanol at concentrations of 55% with 0.5% N,N'-Methylenebisacry-lamide(MBA). Solution(70 μL)was added and spread uniformly over 35 mm petri dish. These dishes were immediately subjected to irradiation. After follow-up treatment, rabbit corneal stromal cells were cultured on thermo-sensitive petri dish in vitro.RESULTS: According to the monomer formula and radiation synthesis scheme in this experiment, PNIPAAm can be synthesized on the surface of the petri dish. Rabbit corneal stromal cells grew well in the thermo- sensitive surface and can be separated into sheets.CONCLUSION: The single and multilayer carrier-free cell sheets can be obtained from the use of thermo-sensitive petri dish. |
| first_indexed | 2024-04-24T05:59:59Z |
| format | Article |
| id | doaj.art-85b72e3d64cd45c1980997fdd64549b3 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 |
| language | English |
| last_indexed | 2024-04-24T05:59:59Z |
| publishDate | 2024-05-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-85b72e3d64cd45c1980997fdd64549b32024-04-23T08:29:23ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51232024-05-0124568669010.3980/j.issn.1672-5123.2024.5.05202405005Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerizationWang Wei0Zhang Pengfei1Chen Chunxia2Qian Li3Chen Jiansu4Department of Ophthalmology, Tongling People's Hospital, Tongling 244000, Anhui Province, ChinaThe First Affiliated Hospital of Wannan Medical College;Yijishan Hospital, Wuhu 241000, Anhui Province, ChinaDepartment of Ophthalmology, Tongling People's Hospital, Tongling 244000, Anhui Province, ChinaDepartment of Ophthalmology, Tongling People's Hospital, Tongling 244000, Anhui Province, ChinaInstitute of Ophthalmology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong Province, ChinaAIM: To explore the synthesis of thermo-sensitive poly N-isopropylacry-lamide(PNIPAAm)and the petri dish grafted with PNIPAAm hydrogels by the electron accelerator, as well as the growth conditions and the biological characteristics of rabbit corneal stromal cells on thermo-sensitive PNIPAAm hydrogels, and the cell sheets obtained from the PNIPAAm hydrogels.METHODS: NIPAAm monomer was dissolved in 2-propanol at concentrations of 55% with 0.5% N,N'-Methylenebisacry-lamide(MBA). Solution(70 μL)was added and spread uniformly over 35 mm petri dish. These dishes were immediately subjected to irradiation. After follow-up treatment, rabbit corneal stromal cells were cultured on thermo-sensitive petri dish in vitro.RESULTS: According to the monomer formula and radiation synthesis scheme in this experiment, PNIPAAm can be synthesized on the surface of the petri dish. Rabbit corneal stromal cells grew well in the thermo- sensitive surface and can be separated into sheets.CONCLUSION: The single and multilayer carrier-free cell sheets can be obtained from the use of thermo-sensitive petri dish.http://ies.ijo.cn/cn_publish/2024/5/202405005.pdfpoly n-isopropylacry-lamide(pnipaam)thermo-sensitive materialscell sheetcorneal stromacell culture |
| spellingShingle | Wang Wei Zhang Pengfei Chen Chunxia Qian Li Chen Jiansu Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization Guoji Yanke Zazhi poly n-isopropylacry-lamide(pnipaam) thermo-sensitive materials cell sheet corneal stroma cell culture |
| title | Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization |
| title_full | Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization |
| title_fullStr | Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization |
| title_full_unstemmed | Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization |
| title_short | Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization |
| title_sort | culture of rabbit corneal stromal cells on thermo sensitive materials by the radiation polymerization |
| topic | poly n-isopropylacry-lamide(pnipaam) thermo-sensitive materials cell sheet corneal stroma cell culture |
| url | http://ies.ijo.cn/cn_publish/2024/5/202405005.pdf |
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