Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress
Hyperosmolality-gated calcium-permeable channels (OSCA) are characterized as an osmosensor in plants; they are able to recognize and respond to exogenous and endogenous osmotic changes, and play a vital role in plant growth and adaptability to environmental stress. To explore the potential biologica...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13658 |
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| author | Yuanyang Li Yubin Zhang Bin Li Liyuan Hou Jianing Yu Chengguo Jia Zhe Wang Siqi Chen Mingzhe Zhang Jianchun Qin Ning Cao Jinhu Cui Wuliang Shi |
| author_facet | Yuanyang Li Yubin Zhang Bin Li Liyuan Hou Jianing Yu Chengguo Jia Zhe Wang Siqi Chen Mingzhe Zhang Jianchun Qin Ning Cao Jinhu Cui Wuliang Shi |
| author_sort | Yuanyang Li |
| collection | DOAJ |
| description | Hyperosmolality-gated calcium-permeable channels (OSCA) are characterized as an osmosensor in plants; they are able to recognize and respond to exogenous and endogenous osmotic changes, and play a vital role in plant growth and adaptability to environmental stress. To explore the potential biological functions of OSCAs in maize, we performed a bioinformatics and expression analysis of the <i>ZmOSCA</i> gene family. Using bioinformatics methods, we identified twelve <i>OSCA</i> genes from the genome database of maize. According to their sequence composition and phylogenetic relationship, the maize <i>OSCA</i> family was classified into four groups (Ⅰ, Ⅱ, Ⅲ, and Ⅳ). Multiple sequence alignment analysis revealed a conserved DUF221 domain in these members. We modeled the calcium binding sites of four OSCA families using the autodocking technique. The expression profiles of <i>ZmOSCA</i> genes were analyzed in different tissues and under diverse abiotic stresses such as drought, salt, high temperature, and chilling using quantitative real-time PCR (qRT-PCR). We found that the expression of twelve <i>ZmOSCA</i> genes is variant in different tissues of maize. Furthermore, abiotic stresses such as drought, salt, high temperature, and chilling differentially induced the expression of twelve <i>ZmOSCA</i> genes. We chose <i>Zm</i>OSCA2.2 and <i>Zm</i>OSCA2.3, which responded most strongly to temperature stress, for prediction of protein interactions. We modeled the calcium binding sites of four OSCA families using autodocking tools, obtaining a number of new results. These results are helpful in understanding the function of the plant <i>OSCA</i> gene family for study of the molecular mechanism of plant osmotic stress and response, as well as exploration of the interaction between osmotic stress, high-temperature stress, and low-temperature stress signal transduction mechanisms. As such, they can provide a theoretical basis for crop breeding. |
| first_indexed | 2024-03-09T18:58:43Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:58:43Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-85b749127034481982b4ad7ddeb7db9c2023-11-24T05:11:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211365810.3390/ijms232113658Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature StressYuanyang Li0Yubin Zhang1Bin Li2Liyuan Hou3Jianing Yu4Chengguo Jia5Zhe Wang6Siqi Chen7Mingzhe Zhang8Jianchun Qin9Ning Cao10Jinhu Cui11Wuliang Shi12Center for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaCenter for Emerging Agricultural Education & Advanced Interdisciplinary Science, College of Plant Science, Jilin University, Changchun 130062, ChinaHyperosmolality-gated calcium-permeable channels (OSCA) are characterized as an osmosensor in plants; they are able to recognize and respond to exogenous and endogenous osmotic changes, and play a vital role in plant growth and adaptability to environmental stress. To explore the potential biological functions of OSCAs in maize, we performed a bioinformatics and expression analysis of the <i>ZmOSCA</i> gene family. Using bioinformatics methods, we identified twelve <i>OSCA</i> genes from the genome database of maize. According to their sequence composition and phylogenetic relationship, the maize <i>OSCA</i> family was classified into four groups (Ⅰ, Ⅱ, Ⅲ, and Ⅳ). Multiple sequence alignment analysis revealed a conserved DUF221 domain in these members. We modeled the calcium binding sites of four OSCA families using the autodocking technique. The expression profiles of <i>ZmOSCA</i> genes were analyzed in different tissues and under diverse abiotic stresses such as drought, salt, high temperature, and chilling using quantitative real-time PCR (qRT-PCR). We found that the expression of twelve <i>ZmOSCA</i> genes is variant in different tissues of maize. Furthermore, abiotic stresses such as drought, salt, high temperature, and chilling differentially induced the expression of twelve <i>ZmOSCA</i> genes. We chose <i>Zm</i>OSCA2.2 and <i>Zm</i>OSCA2.3, which responded most strongly to temperature stress, for prediction of protein interactions. We modeled the calcium binding sites of four OSCA families using autodocking tools, obtaining a number of new results. These results are helpful in understanding the function of the plant <i>OSCA</i> gene family for study of the molecular mechanism of plant osmotic stress and response, as well as exploration of the interaction between osmotic stress, high-temperature stress, and low-temperature stress signal transduction mechanisms. As such, they can provide a theoretical basis for crop breeding.https://www.mdpi.com/1422-0067/23/21/13658calcium signalingOSCADUF221 domainabiotic stressmaize (<i>Zea mays</i> L.) |
| spellingShingle | Yuanyang Li Yubin Zhang Bin Li Liyuan Hou Jianing Yu Chengguo Jia Zhe Wang Siqi Chen Mingzhe Zhang Jianchun Qin Ning Cao Jinhu Cui Wuliang Shi Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress International Journal of Molecular Sciences calcium signaling OSCA DUF221 domain abiotic stress maize (<i>Zea mays</i> L.) |
| title | Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress |
| title_full | Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress |
| title_fullStr | Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress |
| title_full_unstemmed | Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress |
| title_short | Preliminary Expression Analysis of the <i>OSCA</i> Gene Family in Maize and Their Involvement in Temperature Stress |
| title_sort | preliminary expression analysis of the i osca i gene family in maize and their involvement in temperature stress |
| topic | calcium signaling OSCA DUF221 domain abiotic stress maize (<i>Zea mays</i> L.) |
| url | https://www.mdpi.com/1422-0067/23/21/13658 |
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