Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe
Three autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/3/296 |
| _version_ | 1818863599413624832 |
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| author | Guillaume A. Durand Géraldine Piorkowski Laurence Thirion Laetitia Ninove Sandra Giron Christine Zandotti Jessica Denis Cyril Badaut Anna-Bella Failloux Gilda Grard Isabelle Leparc-Goffart Xavier de Lamballerie |
| author_facet | Guillaume A. Durand Géraldine Piorkowski Laurence Thirion Laetitia Ninove Sandra Giron Christine Zandotti Jessica Denis Cyril Badaut Anna-Bella Failloux Gilda Grard Isabelle Leparc-Goffart Xavier de Lamballerie |
| author_sort | Guillaume A. Durand |
| collection | DOAJ |
| description | Three autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested PCR and sequencing in the Pr gene. Analysis of 16 genotype-specific Single Nucleotides Polymorphisms identified the Asian genotype and suggested a Southeast Asia origin. |
| first_indexed | 2024-12-19T10:18:19Z |
| format | Article |
| id | doaj.art-85bc4217a57b4b9c99dd74a2d598a266 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-19T10:18:19Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-85bc4217a57b4b9c99dd74a2d598a2662022-12-21T20:26:09ZengMDPI AGViruses1999-49152020-03-0112329610.3390/v12030296v12030296Vector-Borne Transmission of the Zika Virus Asian Genotype in EuropeGuillaume A. Durand0Géraldine Piorkowski1Laurence Thirion2Laetitia Ninove3Sandra Giron4Christine Zandotti5Jessica Denis6Cyril Badaut7Anna-Bella Failloux8Gilda Grard9Isabelle Leparc-Goffart10Xavier de Lamballerie11National Reference Laboratory for Arboviruses, Institut de Recherche Biomédicale des Armées, 13010 Marseille, FranceUnité des Virus Emergents (UVE: Aix-Marseille Univ–IRD 190–Inserm 1207–IHU Méditerranée Infection), 13010 Marseille, FranceUnité des Virus Emergents (UVE: Aix-Marseille Univ–IRD 190–Inserm 1207–IHU Méditerranée Infection), 13010 Marseille, FranceUnité des Virus Emergents (UVE: Aix-Marseille Univ–IRD 190–Inserm 1207–IHU Méditerranée Infection), 13010 Marseille, FranceFrench National Public Health Agency (Santé publique France), 13002 Marseille, FranceUnité des Virus Emergents (UVE: Aix-Marseille Univ–IRD 190–Inserm 1207–IHU Méditerranée Infection), 13010 Marseille, FranceNational Reference Laboratory for Arboviruses, Institut de Recherche Biomédicale des Armées, 13010 Marseille, FranceNational Reference Laboratory for Arboviruses, Institut de Recherche Biomédicale des Armées, 13010 Marseille, FranceDepartment of Virology, Arboviruses and Insect Vectors, Institut Pasteur, 75015 Paris, FranceNational Reference Laboratory for Arboviruses, Institut de Recherche Biomédicale des Armées, 13010 Marseille, FranceNational Reference Laboratory for Arboviruses, Institut de Recherche Biomédicale des Armées, 13010 Marseille, FranceUnité des Virus Emergents (UVE: Aix-Marseille Univ–IRD 190–Inserm 1207–IHU Méditerranée Infection), 13010 Marseille, FranceThree autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested PCR and sequencing in the Pr gene. Analysis of 16 genotype-specific Single Nucleotides Polymorphisms identified the Asian genotype and suggested a Southeast Asia origin.https://www.mdpi.com/1999-4915/12/3/296arboviruszikafrance<i>aedes albopictus</i>autochthonous transmissiongenotyping |
| spellingShingle | Guillaume A. Durand Géraldine Piorkowski Laurence Thirion Laetitia Ninove Sandra Giron Christine Zandotti Jessica Denis Cyril Badaut Anna-Bella Failloux Gilda Grard Isabelle Leparc-Goffart Xavier de Lamballerie Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe Viruses arbovirus zika france <i>aedes albopictus</i> autochthonous transmission genotyping |
| title | Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe |
| title_full | Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe |
| title_fullStr | Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe |
| title_full_unstemmed | Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe |
| title_short | Vector-Borne Transmission of the Zika Virus Asian Genotype in Europe |
| title_sort | vector borne transmission of the zika virus asian genotype in europe |
| topic | arbovirus zika france <i>aedes albopictus</i> autochthonous transmission genotyping |
| url | https://www.mdpi.com/1999-4915/12/3/296 |
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