Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i>
Mixed cultures represent better alternatives to ferment organic waste and dark fermentation products in anerobic conditions because the microbial associations contribute to electron transfer mechanisms and combine metabolic possibilities. The understanding of the microbial interactions in natural an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/11/644 |
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| author | Axayacatl González Edgar Salgado Zaira Vanegas Cristina Niño-Navarro Omar Cortés Isaac Chairez Elvia I. García-Peña |
| author_facet | Axayacatl González Edgar Salgado Zaira Vanegas Cristina Niño-Navarro Omar Cortés Isaac Chairez Elvia I. García-Peña |
| author_sort | Axayacatl González |
| collection | DOAJ |
| description | Mixed cultures represent better alternatives to ferment organic waste and dark fermentation products in anerobic conditions because the microbial associations contribute to electron transfer mechanisms and combine metabolic possibilities. The understanding of the microbial interactions in natural and synthetic consortia and the strategies to improve the performance of the processes by bioaugmentation provide insight into the physiology and ecology of the mixed cultures used for biotechnological purposes. Here, synthetic microbial communities were built from three hydrogen (bioH<sub>2</sub>) and poly-hydroxy-alkanoates (PHA) producers, <i>Clostridium pasteurianum</i>, <i>Rhodopseudomonas palustris</i> and <i>Syntrophomonas wolfei</i>, and a photoheterotrophic mixed consortium C4, and their performance was evaluated during photofermentation. Higher hydrogen volumetric production rates (H<sub>2</sub>VPR) were determined with the consortia (28–40 mL/Lh) as compared with individual strains (20–27 mL/Lh). The designed consortia reached the highest bioH<sub>2</sub> and PHA productions of 44.3 mmol and 50.46% and produced both metabolites simultaneously using dark fermentation effluents composed of a mixture of lactic, butyric, acetic, and propionic acids. When the mixed culture C4 was bioaugmented with <i>S. wolfei</i>, the bioH<sub>2</sub> and PHA production reached 32 mmol and 50%, respectively. Overall, the consumption of organic acids was above 50%, which accounted up to 55% of total chemical oxygen demand (COD) removed. Increased bioH<sub>2</sub> was observed in the condition when <i>S. wolfei</i> was added as the bioaugmentation agent, reaching up to 562 mL of H<sub>2</sub> produced per gram of COD. The enhanced production of bioH<sub>2</sub> and PHA can be explained by the metabolic interaction between the three selected strains, which likely include thermodynamic equilibrium, the assimilation of organic acids via beta-oxidation, and the production of bioH<sub>2</sub> using a proton driving force derived from reduced menaquinone or via electron bifurcation. |
| first_indexed | 2024-03-09T18:20:43Z |
| format | Article |
| id | doaj.art-85bd1f6c3f124a01b26632a94aea05f1 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T18:20:43Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-85bd1f6c3f124a01b26632a94aea05f12023-11-24T08:19:51ZengMDPI AGFermentation2311-56372022-11-0181164410.3390/fermentation8110644Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i>Axayacatl González0Edgar Salgado1Zaira Vanegas2Cristina Niño-Navarro3Omar Cortés4Isaac Chairez5Elvia I. García-Peña6Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane 4072, AustraliaBioengineering Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Mexico City 07340, MexicoBioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Mexico City 07340, MexicoBioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Mexico City 07340, MexicoBioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Mexico City 07340, MexicoBioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Mexico City 07340, MexicoBioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Mexico City 07340, MexicoMixed cultures represent better alternatives to ferment organic waste and dark fermentation products in anerobic conditions because the microbial associations contribute to electron transfer mechanisms and combine metabolic possibilities. The understanding of the microbial interactions in natural and synthetic consortia and the strategies to improve the performance of the processes by bioaugmentation provide insight into the physiology and ecology of the mixed cultures used for biotechnological purposes. Here, synthetic microbial communities were built from three hydrogen (bioH<sub>2</sub>) and poly-hydroxy-alkanoates (PHA) producers, <i>Clostridium pasteurianum</i>, <i>Rhodopseudomonas palustris</i> and <i>Syntrophomonas wolfei</i>, and a photoheterotrophic mixed consortium C4, and their performance was evaluated during photofermentation. Higher hydrogen volumetric production rates (H<sub>2</sub>VPR) were determined with the consortia (28–40 mL/Lh) as compared with individual strains (20–27 mL/Lh). The designed consortia reached the highest bioH<sub>2</sub> and PHA productions of 44.3 mmol and 50.46% and produced both metabolites simultaneously using dark fermentation effluents composed of a mixture of lactic, butyric, acetic, and propionic acids. When the mixed culture C4 was bioaugmented with <i>S. wolfei</i>, the bioH<sub>2</sub> and PHA production reached 32 mmol and 50%, respectively. Overall, the consumption of organic acids was above 50%, which accounted up to 55% of total chemical oxygen demand (COD) removed. Increased bioH<sub>2</sub> was observed in the condition when <i>S. wolfei</i> was added as the bioaugmentation agent, reaching up to 562 mL of H<sub>2</sub> produced per gram of COD. The enhanced production of bioH<sub>2</sub> and PHA can be explained by the metabolic interaction between the three selected strains, which likely include thermodynamic equilibrium, the assimilation of organic acids via beta-oxidation, and the production of bioH<sub>2</sub> using a proton driving force derived from reduced menaquinone or via electron bifurcation.https://www.mdpi.com/2311-5637/8/11/644bioaugmentationbioH<sub>2</sub>polyhydroxy-alkanoates (PHAs)synthetic microbial consortiumphotoheterotrophic mixed cultures |
| spellingShingle | Axayacatl González Edgar Salgado Zaira Vanegas Cristina Niño-Navarro Omar Cortés Isaac Chairez Elvia I. García-Peña Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i> Fermentation bioaugmentation bioH<sub>2</sub> polyhydroxy-alkanoates (PHAs) synthetic microbial consortium photoheterotrophic mixed cultures |
| title | Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i> |
| title_full | Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i> |
| title_fullStr | Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i> |
| title_full_unstemmed | Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i> |
| title_short | Simultaneous Production of Biohydrogen (bioH<sub>2</sub>) and Poly-Hydroxy-Alkanoates (PHAs) by a Photoheterotrophic Consortium Bioaugmented with <i>Syntrophomonas wolfei</i> |
| title_sort | simultaneous production of biohydrogen bioh sub 2 sub and poly hydroxy alkanoates phas by a photoheterotrophic consortium bioaugmented with i syntrophomonas wolfei i |
| topic | bioaugmentation bioH<sub>2</sub> polyhydroxy-alkanoates (PHAs) synthetic microbial consortium photoheterotrophic mixed cultures |
| url | https://www.mdpi.com/2311-5637/8/11/644 |
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