Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background
Grain shape and size are key determinants of grain appearance quality and yield in rice. In our previous study, a grain shape QTL, <i>qGS1-35.2</i>, was fine-mapped using near-isogenic lines (NILs) derived from a cross between Zhenshan 97 (ZS97) and Milyang 46 (MY46). One annotated gene,...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/19/2530 |
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| author | Zhu-Hao Li Shi-Lin Wang Yu-Jun Zhu Ye-Yang Fan De-Run Huang Ai-Ke Zhu Jie-Yun Zhuang Yan Liang Zhen-Hua Zhang |
| author_facet | Zhu-Hao Li Shi-Lin Wang Yu-Jun Zhu Ye-Yang Fan De-Run Huang Ai-Ke Zhu Jie-Yun Zhuang Yan Liang Zhen-Hua Zhang |
| author_sort | Zhu-Hao Li |
| collection | DOAJ |
| description | Grain shape and size are key determinants of grain appearance quality and yield in rice. In our previous study, a grain shape QTL, <i>qGS1-35.2</i>, was fine-mapped using near-isogenic lines (NILs) derived from a cross between Zhenshan 97 (ZS97) and Milyang 46 (MY46). One annotated gene, <i>OsPUB3</i>, was found to be the most likely candidate gene. Here, knockout and overexpression experiments were performed to investigate the effects of <i>OsPUB3</i> on grain shape and size. Four traits were tested, including grain length, grain width, grain weight, and the ratio of grain length to width. Knockout of <i>OsPUB3</i> in NIL<sup>ZS97</sup>, NIL<sup>MY46</sup>, and another rice cultivar carrying the <i>OsPUB3</i><sup>MY46</sup> allele all caused decreases in grain width and weight and increases in the ratio of grain length to width. Results also showed that the magnitude of the mutational effects varied depending on the target allele and the genetic background. Moreover, it was found that NIL<sup>ZS97</sup> and NIL<sup>MY46</sup> carried different functional alleles of <i>OsPUB3</i>, causing differences in grain shape rather than grain weight. In the overexpression experiment, significant differences between transgenic-positive and transgenic-negative plants were detected in all four traits. These results indicate that <i>OsPUB3</i> regulates grain shape and size through a complex mechanism and is a good target for deciphering the regulatory network of grain shape. This gene could be used to improve grain appearance quality through molecular breeding as well. |
| first_indexed | 2024-03-09T21:17:58Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T21:17:58Z |
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| series | Plants |
| spelling | doaj.art-85be61498ec942b89295b5beb72292ec2023-11-23T21:29:00ZengMDPI AGPlants2223-77472022-09-011119253010.3390/plants11192530Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic BackgroundZhu-Hao Li0Shi-Lin Wang1Yu-Jun Zhu2Ye-Yang Fan3De-Run Huang4Ai-Ke Zhu5Jie-Yun Zhuang6Yan Liang7Zhen-Hua Zhang8State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaNanchong Academy of Agricultural Sciences, Nanchong 637000, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, ChinaGrain shape and size are key determinants of grain appearance quality and yield in rice. In our previous study, a grain shape QTL, <i>qGS1-35.2</i>, was fine-mapped using near-isogenic lines (NILs) derived from a cross between Zhenshan 97 (ZS97) and Milyang 46 (MY46). One annotated gene, <i>OsPUB3</i>, was found to be the most likely candidate gene. Here, knockout and overexpression experiments were performed to investigate the effects of <i>OsPUB3</i> on grain shape and size. Four traits were tested, including grain length, grain width, grain weight, and the ratio of grain length to width. Knockout of <i>OsPUB3</i> in NIL<sup>ZS97</sup>, NIL<sup>MY46</sup>, and another rice cultivar carrying the <i>OsPUB3</i><sup>MY46</sup> allele all caused decreases in grain width and weight and increases in the ratio of grain length to width. Results also showed that the magnitude of the mutational effects varied depending on the target allele and the genetic background. Moreover, it was found that NIL<sup>ZS97</sup> and NIL<sup>MY46</sup> carried different functional alleles of <i>OsPUB3</i>, causing differences in grain shape rather than grain weight. In the overexpression experiment, significant differences between transgenic-positive and transgenic-negative plants were detected in all four traits. These results indicate that <i>OsPUB3</i> regulates grain shape and size through a complex mechanism and is a good target for deciphering the regulatory network of grain shape. This gene could be used to improve grain appearance quality through molecular breeding as well.https://www.mdpi.com/2223-7747/11/19/2530gene cloninggrain shape and size<i>OsPUB3</i>QTLrice |
| spellingShingle | Zhu-Hao Li Shi-Lin Wang Yu-Jun Zhu Ye-Yang Fan De-Run Huang Ai-Ke Zhu Jie-Yun Zhuang Yan Liang Zhen-Hua Zhang Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background Plants gene cloning grain shape and size <i>OsPUB3</i> QTL rice |
| title | Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background |
| title_full | Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background |
| title_fullStr | Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background |
| title_full_unstemmed | Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background |
| title_short | Control of Grain Shape and Size in Rice by Two Functional Alleles of <i>OsPUB3</i> in Varied Genetic Background |
| title_sort | control of grain shape and size in rice by two functional alleles of i ospub3 i in varied genetic background |
| topic | gene cloning grain shape and size <i>OsPUB3</i> QTL rice |
| url | https://www.mdpi.com/2223-7747/11/19/2530 |
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