Deficient spermiogenesis in mice lacking Rlim
The X-linked gene Rlim plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation in extraembryonic tissues of embryos. However, while females carrying a systemic Rlim knockout (KO) die around implantation, male Rlim...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/63556 |
| _version_ | 1811202634766352384 |
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| author | Feng Wang Maria Gracia Gervasi Ana Bošković Fengyun Sun Vera D Rinaldi Jun Yu Mary C Wallingford Darya A Tourzani Jesse Mager Lihua Julie Zhu Oliver J Rando Pablo E Visconti Lara Strittmatter Ingolf Bach |
| author_facet | Feng Wang Maria Gracia Gervasi Ana Bošković Fengyun Sun Vera D Rinaldi Jun Yu Mary C Wallingford Darya A Tourzani Jesse Mager Lihua Julie Zhu Oliver J Rando Pablo E Visconti Lara Strittmatter Ingolf Bach |
| author_sort | Feng Wang |
| collection | DOAJ |
| description | The X-linked gene Rlim plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation in extraembryonic tissues of embryos. However, while females carrying a systemic Rlim knockout (KO) die around implantation, male Rlim KO mice appear healthy and are fertile. Here, we report an important role for Rlim in testis where it is highly expressed in post-meiotic round spermatids as well as in Sertoli cells. Systemic deletion of the Rlim gene results in lower numbers of mature sperm that contains excess cytoplasm, leading to decreased sperm motility and in vitro fertilization rates. Targeting the conditional Rlim cKO specifically to the spermatogenic cell lineage largely recapitulates this phenotype. These results reveal functions of Rlim in male reproduction specifically in round spermatids during spermiogenesis. |
| first_indexed | 2024-04-12T02:42:20Z |
| format | Article |
| id | doaj.art-85c0317343a64841b13715f36fcb3890 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:42:20Z |
| publishDate | 2021-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-85c0317343a64841b13715f36fcb38902022-12-22T03:51:17ZengeLife Sciences Publications LtdeLife2050-084X2021-02-011010.7554/eLife.63556Deficient spermiogenesis in mice lacking RlimFeng Wang0Maria Gracia Gervasi1https://orcid.org/0000-0002-5468-2700Ana Bošković2Fengyun Sun3Vera D Rinaldi4https://orcid.org/0000-0002-0051-1754Jun Yu5Mary C Wallingford6Darya A Tourzani7Jesse Mager8Lihua Julie Zhu9Oliver J Rando10https://orcid.org/0000-0003-1516-9397Pablo E Visconti11Lara Strittmatter12Ingolf Bach13https://orcid.org/0000-0003-4505-8946Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Veterinary & Animal Sciences, University of Massachusetts Amherst, Amherst, United StatesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Veterinary & Animal Sciences, University of Massachusetts Amherst, Amherst, United StatesDepartment of Veterinary & Animal Sciences, University of Massachusetts Amherst, Amherst, United StatesDepartment of Veterinary & Animal Sciences, University of Massachusetts Amherst, Amherst, United StatesDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, United States; Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Veterinary & Animal Sciences, University of Massachusetts Amherst, Amherst, United StatesElectron Microscopy Core, University of Massachusetts Medical School, Worcester, United StatesDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, United StatesThe X-linked gene Rlim plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation in extraembryonic tissues of embryos. However, while females carrying a systemic Rlim knockout (KO) die around implantation, male Rlim KO mice appear healthy and are fertile. Here, we report an important role for Rlim in testis where it is highly expressed in post-meiotic round spermatids as well as in Sertoli cells. Systemic deletion of the Rlim gene results in lower numbers of mature sperm that contains excess cytoplasm, leading to decreased sperm motility and in vitro fertilization rates. Targeting the conditional Rlim cKO specifically to the spermatogenic cell lineage largely recapitulates this phenotype. These results reveal functions of Rlim in male reproduction specifically in round spermatids during spermiogenesis.https://elifesciences.org/articles/63556Rlimspermiogenesiscytoplasmic reductionmouse genetics |
| spellingShingle | Feng Wang Maria Gracia Gervasi Ana Bošković Fengyun Sun Vera D Rinaldi Jun Yu Mary C Wallingford Darya A Tourzani Jesse Mager Lihua Julie Zhu Oliver J Rando Pablo E Visconti Lara Strittmatter Ingolf Bach Deficient spermiogenesis in mice lacking Rlim eLife Rlim spermiogenesis cytoplasmic reduction mouse genetics |
| title | Deficient spermiogenesis in mice lacking Rlim |
| title_full | Deficient spermiogenesis in mice lacking Rlim |
| title_fullStr | Deficient spermiogenesis in mice lacking Rlim |
| title_full_unstemmed | Deficient spermiogenesis in mice lacking Rlim |
| title_short | Deficient spermiogenesis in mice lacking Rlim |
| title_sort | deficient spermiogenesis in mice lacking rlim |
| topic | Rlim spermiogenesis cytoplasmic reduction mouse genetics |
| url | https://elifesciences.org/articles/63556 |
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