Pre-Growth Culture Conditions Affect Type 1 Fimbriae-Dependent Adhesion of <i>Salmonella</i>
Among various fimbrial structures used by <i>Salmonella enterica</i> to colonize host tissues, type 1 fimbriae (T1F) are among the most extensively studied. Although some experiments have shown the importance of T1F in the initial stages of <i>Salmonella</i> infection, their...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/12/4206 |
| Summary: | Among various fimbrial structures used by <i>Salmonella enterica</i> to colonize host tissues, type 1 fimbriae (T1F) are among the most extensively studied. Although some experiments have shown the importance of T1F in the initial stages of <i>Salmonella</i> infection, their exact role in the infection process is not fully known. We suggested that different outcomes of T1F investigations were due to the use of different pre-infection growth conditions for the induction of the T1F. We utilized qPCR, flow cytometry, and a wide range of adhesion assays to investigate <i>Salmonella</i> Choleraesuis and <i>Salmonella</i> Typhimurium adhesion in the context of T1F expression. We demonstrated that T1F expression was highly dependent on the pre-infection growth conditions. These growth conditions yielded T1F+ and T1F- populations of <i>Salmonella</i> and, therefore, could be a factor influencing <i>Salmonella-</i>host cell interactions. We supported this conclusion by showing that increased levels of T1F expression directly correlated with higher levels of <i>Salmonella</i> adherence to the intestinal epithelial IPEC-J2 cell line. |
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| ISSN: | 1661-6596 1422-0067 |