Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method
This study was designed to elucidate the immunoregulation of Ganoderma lucidum. HPLC fingerprint and spectrum-effect relationship of G. lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy. Then the target compounds were identif...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2021-05-01
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| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000288 |
| _version_ | 1797728907360731136 |
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| author | Changqin Li Yiping Cui Jie Lu Lijun Meng Changyang Ma Zhenhua Liu Yan Zhang Wenyi Kang |
| author_facet | Changqin Li Yiping Cui Jie Lu Lijun Meng Changyang Ma Zhenhua Liu Yan Zhang Wenyi Kang |
| author_sort | Changqin Li |
| collection | DOAJ |
| description | This study was designed to elucidate the immunoregulation of Ganoderma lucidum. HPLC fingerprint and spectrum-effect relationship of G. lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy. Then the target compounds were identified by high resolution mass spectrometry. The results indicated that there are both enhanced immunity and immunosuppressive components in G. lucidum. BP neural network was trained with the common peak area and immune efficacy index of G. lucidum fingerprint as samples, and a combined evaluation system of G. lucidum fingerprint efficacy was established. The correlation coefficient R of BP network model was 0.986 43, and the error of pharmacodynamic prediction results was in the ideal range. Eight compounds were identified by high resolution mass spectrometry. The compounds related to immune activity in G. lucidum were determined in this study. |
| first_indexed | 2024-03-12T11:20:55Z |
| format | Article |
| id | doaj.art-85c95482adad4560a8e4ee3ca9498b77 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T11:20:55Z |
| publishDate | 2021-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-85c95482adad4560a8e4ee3ca9498b772023-09-02T00:04:25ZengTsinghua University PressFood Science and Human Wellness2213-45302021-05-01103278288Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out methodChangqin Li0Yiping Cui1Jie Lu2Lijun Meng3Changyang Ma4Zhenhua Liu5Yan Zhang6Wenyi Kang7National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Henan Province, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Henan Province, Kaifeng, 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Henan Province, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Henan Province, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng 475004, China; Corresponding author.Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China; Corresponding author.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China; Functional Food Engineering Technology Research Center, Henan Province, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng 475004, China; Corresponding author.This study was designed to elucidate the immunoregulation of Ganoderma lucidum. HPLC fingerprint and spectrum-effect relationship of G. lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy. Then the target compounds were identified by high resolution mass spectrometry. The results indicated that there are both enhanced immunity and immunosuppressive components in G. lucidum. BP neural network was trained with the common peak area and immune efficacy index of G. lucidum fingerprint as samples, and a combined evaluation system of G. lucidum fingerprint efficacy was established. The correlation coefficient R of BP network model was 0.986 43, and the error of pharmacodynamic prediction results was in the ideal range. Eight compounds were identified by high resolution mass spectrometry. The compounds related to immune activity in G. lucidum were determined in this study.http://www.sciencedirect.com/science/article/pii/S2213453021000288Ganoderma lucidumRAW 264.7 cellSpectrum-effect relationshipsComponent knock-outBP neural network |
| spellingShingle | Changqin Li Yiping Cui Jie Lu Lijun Meng Changyang Ma Zhenhua Liu Yan Zhang Wenyi Kang Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method Food Science and Human Wellness Ganoderma lucidum RAW 264.7 cell Spectrum-effect relationships Component knock-out BP neural network |
| title | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method |
| title_full | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method |
| title_fullStr | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method |
| title_full_unstemmed | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method |
| title_short | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method |
| title_sort | spectrum effect relationship of immunologic activity of ganoderma lucidum by uplc ms ms and component knock out method |
| topic | Ganoderma lucidum RAW 264.7 cell Spectrum-effect relationships Component knock-out BP neural network |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000288 |
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