Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin
Erysipelas is a re-emerging disease in different poultry species. Antibiotic treatment is crucial to combat outbreaks in poultry flocks, but only very limited data on susceptibility are available. Recently, the Clinical and Laboratory Standard Institute established standardized guidelines and minima...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Serija: | Poultry |
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| Online dostop: | https://www.mdpi.com/2674-1164/2/1/7 |
| _version_ | 1827747941790515200 |
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| author | Claudia Hess Ivana Bilic Delfina Jandreski-Cvetkovic Michael Hess |
| author_facet | Claudia Hess Ivana Bilic Delfina Jandreski-Cvetkovic Michael Hess |
| author_sort | Claudia Hess |
| collection | DOAJ |
| description | Erysipelas is a re-emerging disease in different poultry species. Antibiotic treatment is crucial to combat outbreaks in poultry flocks, but only very limited data on susceptibility are available. Recently, the Clinical and Laboratory Standard Institute established standardized guidelines and minimal inhibitory concentration breakpoints for <i>E. rhusiopathiae</i> when using the broth microdilution method. In the present investigation, these guidelines were applied to evaluate the antimicrobial susceptibility of 30 <i>E. rhusiopathiae</i> isolates derived from field outbreaks in poultry flocks towards penicillins, macrolides, lincosamides and fluoroquinolones. All isolates were identified by MALDI-TOF MS. The majority of isolates belonged to two serovars, 1b and 5. More than 40% of the isolates proved resistant to penicillin G, with values ranging from 0.25 to 8 µg/mL. Furthermore, the majority of isolates were found resistant to erythromycin (76.7%; MIC 2–4 µg/mL) and enrofloxacin (60.0%; MIC ≥ 2 µg/mL), altogether limiting treatment options. In contrast, most of the isolates proved susceptible to ampicillin and ceftiofur with MICs ≤ 0.25 µg/mL and ≤2 µg/mL, respectively. A great variety of antimicrobial resistance patterns was found, and multidrug resistance was detected in one-third of the isolates. The presented data are helpful to raise awareness for the antimicrobial resistance of a zoonotic pathogen in context of the One Health concept. |
| first_indexed | 2024-03-11T05:59:18Z |
| format | Article |
| id | doaj.art-85cb151211ef43e4b9a49695a30c047a |
| institution | Directory Open Access Journal |
| issn | 2674-1164 |
| language | English |
| last_indexed | 2024-03-11T05:59:18Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Poultry |
| spelling | doaj.art-85cb151211ef43e4b9a49695a30c047a2023-11-17T13:27:51ZengMDPI AGPoultry2674-11642023-02-0121546210.3390/poultry2010007Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and EnrofloxacinClaudia Hess0Ivana Bilic1Delfina Jandreski-Cvetkovic2Michael Hess3Clinic for Poultry and Fish Medicine, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaClinic for Poultry and Fish Medicine, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaClinic for Poultry and Fish Medicine, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaClinic for Poultry and Fish Medicine, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, AustriaErysipelas is a re-emerging disease in different poultry species. Antibiotic treatment is crucial to combat outbreaks in poultry flocks, but only very limited data on susceptibility are available. Recently, the Clinical and Laboratory Standard Institute established standardized guidelines and minimal inhibitory concentration breakpoints for <i>E. rhusiopathiae</i> when using the broth microdilution method. In the present investigation, these guidelines were applied to evaluate the antimicrobial susceptibility of 30 <i>E. rhusiopathiae</i> isolates derived from field outbreaks in poultry flocks towards penicillins, macrolides, lincosamides and fluoroquinolones. All isolates were identified by MALDI-TOF MS. The majority of isolates belonged to two serovars, 1b and 5. More than 40% of the isolates proved resistant to penicillin G, with values ranging from 0.25 to 8 µg/mL. Furthermore, the majority of isolates were found resistant to erythromycin (76.7%; MIC 2–4 µg/mL) and enrofloxacin (60.0%; MIC ≥ 2 µg/mL), altogether limiting treatment options. In contrast, most of the isolates proved susceptible to ampicillin and ceftiofur with MICs ≤ 0.25 µg/mL and ≤2 µg/mL, respectively. A great variety of antimicrobial resistance patterns was found, and multidrug resistance was detected in one-third of the isolates. The presented data are helpful to raise awareness for the antimicrobial resistance of a zoonotic pathogen in context of the One Health concept.https://www.mdpi.com/2674-1164/2/1/7antibioticssusceptibilityerysipelasOne Health |
| spellingShingle | Claudia Hess Ivana Bilic Delfina Jandreski-Cvetkovic Michael Hess Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin Poultry antibiotics susceptibility erysipelas One Health |
| title | Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin |
| title_full | Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin |
| title_fullStr | Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin |
| title_full_unstemmed | Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin |
| title_short | Antimicrobial Dilution Susceptibility Testing of <i>Erysipelothrix rhusiopathiae</i> According to CLSI Document VET06 Reveals High Resistance against Penicillin G, Erythromycin and Enrofloxacin |
| title_sort | antimicrobial dilution susceptibility testing of i erysipelothrix rhusiopathiae i according to clsi document vet06 reveals high resistance against penicillin g erythromycin and enrofloxacin |
| topic | antibiotics susceptibility erysipelas One Health |
| url | https://www.mdpi.com/2674-1164/2/1/7 |
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