Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity
Abstract Camellia osmantha is a new species of the genus Camellia and is an economically important ornamental plant. Its activity and ingredients are less studied than other Camellia plants. This study investigated the antithrombotic effect and chemical components of C. osmantha fruit cores using pl...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.2769 |
| _version_ | 1798029520071032832 |
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| author | Li Yang Gui‐Liang Xie Jin‐Lin Ma Xiao‐Qiong Huang Yao Gu Lei Huang Hai‐Yan Chen Xi‐Lin Ouyang |
| author_facet | Li Yang Gui‐Liang Xie Jin‐Lin Ma Xiao‐Qiong Huang Yao Gu Lei Huang Hai‐Yan Chen Xi‐Lin Ouyang |
| author_sort | Li Yang |
| collection | DOAJ |
| description | Abstract Camellia osmantha is a new species of the genus Camellia and is an economically important ornamental plant. Its activity and ingredients are less studied than other Camellia plants. This study investigated the antithrombotic effect and chemical components of C. osmantha fruit cores using platelet aggregation assays and coagulation function tests. The cores of C. osmantha fruits were extracted with ethanol to obtain a crude extract. The extract was dissolved in water and further eluted with different concentrations of methanol on an MCI resin column to obtain three fractions. These samples were used for antithrombotic activity tests and phytochemical analysis. The results showed that the extract and its fractions of C. osmantha have strong antithrombotic activity, significantly reducing the platelet aggregation rate and prolonging the thrombin time (TT). The total saponins, flavonoids, and polyphenols in the active fractions may be responsible for the antithrombotic activity. The chemical constituents were analyzed by ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF/MS). Twenty‐three compounds were identified rapidly and accurately. Among them, ellagic acid, naringenin, and quercetin 3‐O‐glucuronide may be important antithrombotic constituents. Furthermore, interactions between these compounds and the P2Y1 receptor were investigated via molecular modeling, because the P2Y1 receptor is a key drug target of antiplatelet aggregative activity. The molecular docking results suggested that these compounds could combine tightly with the P2Y1R protein. Our results showed that C. osmantha fruit cores are rich in polyphenols, flavonoids, and saponins, which can be developed into a promising antithrombotic functional beverage for the prevention and treatment of cardiovascular and cerebrovascular diseases. |
| first_indexed | 2024-04-11T19:26:25Z |
| format | Article |
| id | doaj.art-85cd7649a49749eead37795113f800e3 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-11T19:26:25Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-85cd7649a49749eead37795113f800e32022-12-22T04:07:08ZengWileyFood Science & Nutrition2048-71772022-05-011051510151910.1002/fsn3.2769Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activityLi Yang0Gui‐Liang Xie1Jin‐Lin Ma2Xiao‐Qiong Huang3Yao Gu4Lei Huang5Hai‐Yan Chen6Xi‐Lin Ouyang7Guangxi Key Laboratory of Special Non‐wood Forest Cultivation and Utilization Guangxi Zhuang Autonomous Region Forestry Research Institute Nanning ChinaDepartment of Pharmacy Gannan Healthcare Vocational College Ganzhou ChinaGuangxi Key Laboratory of Special Non‐wood Forest Cultivation and Utilization Guangxi Zhuang Autonomous Region Forestry Research Institute Nanning ChinaDepartment of Pharmacy Gannan Healthcare Vocational College Ganzhou ChinaGuangxi Key Laboratory of Special Non‐wood Forest Cultivation and Utilization Guangxi Zhuang Autonomous Region Forestry Research Institute Nanning ChinaCollege of Public Health and Management Youjiang Medical University for Nationalities Baise ChinaGuangxi Key Laboratory of Special Non‐wood Forest Cultivation and Utilization Guangxi Zhuang Autonomous Region Forestry Research Institute Nanning ChinaDepartment of Pharmacy Gannan Healthcare Vocational College Ganzhou ChinaAbstract Camellia osmantha is a new species of the genus Camellia and is an economically important ornamental plant. Its activity and ingredients are less studied than other Camellia plants. This study investigated the antithrombotic effect and chemical components of C. osmantha fruit cores using platelet aggregation assays and coagulation function tests. The cores of C. osmantha fruits were extracted with ethanol to obtain a crude extract. The extract was dissolved in water and further eluted with different concentrations of methanol on an MCI resin column to obtain three fractions. These samples were used for antithrombotic activity tests and phytochemical analysis. The results showed that the extract and its fractions of C. osmantha have strong antithrombotic activity, significantly reducing the platelet aggregation rate and prolonging the thrombin time (TT). The total saponins, flavonoids, and polyphenols in the active fractions may be responsible for the antithrombotic activity. The chemical constituents were analyzed by ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF/MS). Twenty‐three compounds were identified rapidly and accurately. Among them, ellagic acid, naringenin, and quercetin 3‐O‐glucuronide may be important antithrombotic constituents. Furthermore, interactions between these compounds and the P2Y1 receptor were investigated via molecular modeling, because the P2Y1 receptor is a key drug target of antiplatelet aggregative activity. The molecular docking results suggested that these compounds could combine tightly with the P2Y1R protein. Our results showed that C. osmantha fruit cores are rich in polyphenols, flavonoids, and saponins, which can be developed into a promising antithrombotic functional beverage for the prevention and treatment of cardiovascular and cerebrovascular diseases.https://doi.org/10.1002/fsn3.2769anticoagulantCamellia osmanthaflavonoidspolyphenolssaponinsUPLC‐QTOF/MS |
| spellingShingle | Li Yang Gui‐Liang Xie Jin‐Lin Ma Xiao‐Qiong Huang Yao Gu Lei Huang Hai‐Yan Chen Xi‐Lin Ouyang Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity Food Science & Nutrition anticoagulant Camellia osmantha flavonoids polyphenols saponins UPLC‐QTOF/MS |
| title | Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity |
| title_full | Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity |
| title_fullStr | Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity |
| title_full_unstemmed | Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity |
| title_short | Phytochemical constituents of Camellia osmantha fruit cores with antithrombotic activity |
| title_sort | phytochemical constituents of camellia osmantha fruit cores with antithrombotic activity |
| topic | anticoagulant Camellia osmantha flavonoids polyphenols saponins UPLC‐QTOF/MS |
| url | https://doi.org/10.1002/fsn3.2769 |
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