Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i>
Selenium (Se) is an essential trace element for fish with more than 40 selenoproteins identified, many exhibiting antioxidant functions. This study investigated the effect of dietary Se supplementation on physiological parameters, selenoprotein and antioxidant enzyme gene expression in Atlantic blue...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/26 |
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| author | Pauline Wischhusen Mónica B. Betancor Matthew Sprague Aurelio Ortega Fernando de la Gándara Douglas R. Tocher Gabriel Mourente |
| author_facet | Pauline Wischhusen Mónica B. Betancor Matthew Sprague Aurelio Ortega Fernando de la Gándara Douglas R. Tocher Gabriel Mourente |
| author_sort | Pauline Wischhusen |
| collection | DOAJ |
| description | Selenium (Se) is an essential trace element for fish with more than 40 selenoproteins identified, many exhibiting antioxidant functions. This study investigated the effect of dietary Se supplementation on physiological parameters, selenoprotein and antioxidant enzyme gene expression in Atlantic bluefin tuna (ABT, <i>Thunnus thynnus</i>) larvae. First-feeding ABT larvae were divided into triplicate groups and fed rotifers <i>Brachionus rotundiformis</i> enriched with five different levels of Se (0, 3, 10, 30, and 100 µg Se·L<sup>−1</sup>) until 14 days after hatching. Both rotifers and ABT larvae effectively accumulated Se achieving maximum levels in the Se100 treatment (30.05 μg Se·g<sup>−1</sup> and 194 ± 38 μg Se·g<sup>−1</sup> dry mass, respectively). Larvae showed highest total length when fed Se3 rotifers, whereas flexion index was highest in larvae fed Se10. Selenium supplementation increased the gene expression of selenoproteins <i>gpx1</i>, <i>msrb1, trxr2</i>, <i>selenom</i>, <i>selenop</i>, and <i>selenoe</i> compared to the non-supplemented control (Se0), but only marginal differences were detected between supplementation levels. In contrast, expression of the antioxidant enzymes <i>cat</i> and <i>sod1</i> were lowest in larvae fed Se100. To conclude, non-Se-enriched rotifers may be suboptimal for first feeding ABT larvae, which showed improved selenoprotein and antioxidant gene expression when fed a diet containing 4.42 μg Se·g<sup>−1</sup> dry mass. |
| first_indexed | 2024-03-09T13:47:17Z |
| format | Article |
| id | doaj.art-85cde19c62c04692a816468345b214a5 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:47:17Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-85cde19c62c04692a816468345b214a52023-11-30T20:57:17ZengMDPI AGAntioxidants2076-39212022-12-011212610.3390/antiox12010026Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i>Pauline Wischhusen0Mónica B. Betancor1Matthew Sprague2Aurelio Ortega3Fernando de la Gándara4Douglas R. Tocher5Gabriel Mourente6Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UKInstitute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UKInstitute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UKPlanta Experimental de Cultivos Marinos, Instituto Español de Oceanografía (IEO), 30860 Puerto de Mazarrón (Murcia), SpainPlanta Experimental de Cultivos Marinos, Instituto Español de Oceanografía (IEO), 30860 Puerto de Mazarrón (Murcia), SpainInstitute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UKDepartamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, 11510 Puerto Real, Cádiz, SpainSelenium (Se) is an essential trace element for fish with more than 40 selenoproteins identified, many exhibiting antioxidant functions. This study investigated the effect of dietary Se supplementation on physiological parameters, selenoprotein and antioxidant enzyme gene expression in Atlantic bluefin tuna (ABT, <i>Thunnus thynnus</i>) larvae. First-feeding ABT larvae were divided into triplicate groups and fed rotifers <i>Brachionus rotundiformis</i> enriched with five different levels of Se (0, 3, 10, 30, and 100 µg Se·L<sup>−1</sup>) until 14 days after hatching. Both rotifers and ABT larvae effectively accumulated Se achieving maximum levels in the Se100 treatment (30.05 μg Se·g<sup>−1</sup> and 194 ± 38 μg Se·g<sup>−1</sup> dry mass, respectively). Larvae showed highest total length when fed Se3 rotifers, whereas flexion index was highest in larvae fed Se10. Selenium supplementation increased the gene expression of selenoproteins <i>gpx1</i>, <i>msrb1, trxr2</i>, <i>selenom</i>, <i>selenop</i>, and <i>selenoe</i> compared to the non-supplemented control (Se0), but only marginal differences were detected between supplementation levels. In contrast, expression of the antioxidant enzymes <i>cat</i> and <i>sod1</i> were lowest in larvae fed Se100. To conclude, non-Se-enriched rotifers may be suboptimal for first feeding ABT larvae, which showed improved selenoprotein and antioxidant gene expression when fed a diet containing 4.42 μg Se·g<sup>−1</sup> dry mass.https://www.mdpi.com/2076-3921/12/1/26bluefin tunalarvaeSe-yeastrotifer enrichmentgrowth performancegene expression |
| spellingShingle | Pauline Wischhusen Mónica B. Betancor Matthew Sprague Aurelio Ortega Fernando de la Gándara Douglas R. Tocher Gabriel Mourente Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i> Antioxidants bluefin tuna larvae Se-yeast rotifer enrichment growth performance gene expression |
| title | Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i> |
| title_full | Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i> |
| title_fullStr | Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i> |
| title_full_unstemmed | Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i> |
| title_short | Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (<i>Thunnus Thynnus</i>, <i>L</i>.) Larvae Fed Selenium-Enriched Rotifers <i>Brachionus Rotundiformis</i> |
| title_sort | molecular antioxidant functions are enhanced in atlantic bluefin tuna i thunnus thynnus i i l i larvae fed selenium enriched rotifers i brachionus rotundiformis i |
| topic | bluefin tuna larvae Se-yeast rotifer enrichment growth performance gene expression |
| url | https://www.mdpi.com/2076-3921/12/1/26 |
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