Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves
Abstract This research investigates the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Chromolaena odorata (Asteraceae) and their potential as antibacterial and antifungal agents. Characterization techniques like ultraviolet–visible, Fourier transform infrared (FTIR), D...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-57972-5 |
| _version_ | 1797233601649049600 |
|---|---|
| author | Ajit Kumar Bishoyi Chita Ranjan Sahoo Priyanka Samal Nilima Priyadarsini Mishra Bigyan Ranjan Jali Mohd Shahnawaz Khan Rabindra Nath Padhy |
| author_facet | Ajit Kumar Bishoyi Chita Ranjan Sahoo Priyanka Samal Nilima Priyadarsini Mishra Bigyan Ranjan Jali Mohd Shahnawaz Khan Rabindra Nath Padhy |
| author_sort | Ajit Kumar Bishoyi |
| collection | DOAJ |
| description | Abstract This research investigates the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Chromolaena odorata (Asteraceae) and their potential as antibacterial and antifungal agents. Characterization techniques like ultraviolet–visible, Fourier transform infrared (FTIR), Dynamic light scattering and zeta potential (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), and field emission scanning electron microscopy and energy-dispersive X-ray spectroscopy (FESEM-EDX) confirmed the formation of spherical (AgNPs). UV–vis spectroscopy reaffirms AgNP formation with a peak at 429 nm. DLS and zeta potential measurements revealed an average size of 30.77 nm and a negative surface charge (− 0.532 mV). Further, XRD analysis established the crystalline structure of the AgNPs. Moreover, the TEM descriptions indicate that the AgNPs are spherical shapes, and their sizes ranged from 9 to 22 nm with an average length of 15.27 nm. The X-ray photoelectron spectroscopy (XPS) analysis validated the formation of metallic silver and elucidated the surface state composition of AgNPs. Biologically, CO-AgNPs showed moderate antibacterial activity but excellent antifungal activity against Candida tropicalis (MCC 1559) and Trichophyton rubrum (MCC 1598). Low MIC values (0.195 and 0.390 mg/mL) respectively, suggest their potential as effective antifungal agents. This suggests potential applications in controlling fungal infections, which are often more challenging to treat than bacterial infections. Molecular docking results validated that bioactive compounds in C. odorata contribute to antifungal activity by interacting with its specific domain. Further research could pave the way for the development of novel and safe antifungal therapies based on biogenic nanoparticles. |
| first_indexed | 2024-04-24T16:18:46Z |
| format | Article |
| id | doaj.art-85cffba252364017b3e7c57d42693a42 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T16:18:46Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-85cffba252364017b3e7c57d42693a422024-03-31T11:19:56ZengNature PortfolioScientific Reports2045-23222024-03-0114111510.1038/s41598-024-57972-5Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leavesAjit Kumar Bishoyi0Chita Ranjan Sahoo1Priyanka Samal2Nilima Priyadarsini Mishra3Bigyan Ranjan Jali4Mohd Shahnawaz Khan5Rabindra Nath Padhy6Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan Deemed to be UniversityCentral Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan Deemed to be UniversityDepartment of Clinical Hematology, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan Deemed to be UniversityDepartment of Biochemistry, IIT RoparDepartment of Chemistry, Veer Surendra Sai University of TechnologyDepartment of Biochemistry, College of Science, King Saud UniversityCentral Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha ‘O’ Anusandhan Deemed to be UniversityAbstract This research investigates the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Chromolaena odorata (Asteraceae) and their potential as antibacterial and antifungal agents. Characterization techniques like ultraviolet–visible, Fourier transform infrared (FTIR), Dynamic light scattering and zeta potential (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), and field emission scanning electron microscopy and energy-dispersive X-ray spectroscopy (FESEM-EDX) confirmed the formation of spherical (AgNPs). UV–vis spectroscopy reaffirms AgNP formation with a peak at 429 nm. DLS and zeta potential measurements revealed an average size of 30.77 nm and a negative surface charge (− 0.532 mV). Further, XRD analysis established the crystalline structure of the AgNPs. Moreover, the TEM descriptions indicate that the AgNPs are spherical shapes, and their sizes ranged from 9 to 22 nm with an average length of 15.27 nm. The X-ray photoelectron spectroscopy (XPS) analysis validated the formation of metallic silver and elucidated the surface state composition of AgNPs. Biologically, CO-AgNPs showed moderate antibacterial activity but excellent antifungal activity against Candida tropicalis (MCC 1559) and Trichophyton rubrum (MCC 1598). Low MIC values (0.195 and 0.390 mg/mL) respectively, suggest their potential as effective antifungal agents. This suggests potential applications in controlling fungal infections, which are often more challenging to treat than bacterial infections. Molecular docking results validated that bioactive compounds in C. odorata contribute to antifungal activity by interacting with its specific domain. Further research could pave the way for the development of novel and safe antifungal therapies based on biogenic nanoparticles.https://doi.org/10.1038/s41598-024-57972-5Green synthesisSilver nanoparticlesChromolaena odorataAntimicrobial activitiesMolecular docking |
| spellingShingle | Ajit Kumar Bishoyi Chita Ranjan Sahoo Priyanka Samal Nilima Priyadarsini Mishra Bigyan Ranjan Jali Mohd Shahnawaz Khan Rabindra Nath Padhy Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves Scientific Reports Green synthesis Silver nanoparticles Chromolaena odorata Antimicrobial activities Molecular docking |
| title | Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves |
| title_full | Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves |
| title_fullStr | Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves |
| title_full_unstemmed | Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves |
| title_short | Unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from Chromolaena odorata leaves |
| title_sort | unveiling the antibacterial and antifungal potential of biosynthesized silver nanoparticles from chromolaena odorata leaves |
| topic | Green synthesis Silver nanoparticles Chromolaena odorata Antimicrobial activities Molecular docking |
| url | https://doi.org/10.1038/s41598-024-57972-5 |
| work_keys_str_mv | AT ajitkumarbishoyi unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves AT chitaranjansahoo unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves AT priyankasamal unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves AT nilimapriyadarsinimishra unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves AT bigyanranjanjali unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves AT mohdshahnawazkhan unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves AT rabindranathpadhy unveilingtheantibacterialandantifungalpotentialofbiosynthesizedsilvernanoparticlesfromchromolaenaodorataleaves |