The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production
There is an increasing demand for bio-based polymers that are developed from recycled materials. The production of biodegradable polymers can include bio-technological (utilizing microorganisms or enzymes) or chemical synthesis procedures. This report demonstrates the corroboration of the molecular...
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| Format: | Article |
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MDPI AG
2017-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/4/3/73 |
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| author | Brian Johnston Guozhan Jiang David Hill Grazyna Adamus Iwona Kwiecień Magdalena Zięba Wanda Sikorska Matthew Green Marek Kowalczuk Iza Radecka |
| author_facet | Brian Johnston Guozhan Jiang David Hill Grazyna Adamus Iwona Kwiecień Magdalena Zięba Wanda Sikorska Matthew Green Marek Kowalczuk Iza Radecka |
| author_sort | Brian Johnston |
| collection | DOAJ |
| description | There is an increasing demand for bio-based polymers that are developed from recycled materials. The production of biodegradable polymers can include bio-technological (utilizing microorganisms or enzymes) or chemical synthesis procedures. This report demonstrates the corroboration of the molecular structure of polyhydroxyalkanoates (PHAs) obtained by the conversion of waste polyethylene (PE) via non-oxygenated PE wax (N-PEW) as an additional carbon source for a bacterial species. The N-PEW, obtained from a PE pyrolysis reaction, has been found to be a beneficial carbon source for PHA production with Cupriavidus necator H16. The production of the N-PEW is an alternative to oxidized polyethylene wax (O-PEW) (that has been used as a carbon source previously) as it is less time consuming to manufacture and offers fewer industrial applications. A range of molecular structural analytical techniques were performed on the PHAs obtained; which included nuclear magnetic resonance (NMR) and electrospray ionisation tandem mass spectrometry (ESI-MS/MS). Our study showed that the PHA formed from N-PEW contained 3-hydroxybutyrate (HB) with 11 mol% of 3-hydroxyvalerate (HV) units. |
| first_indexed | 2024-03-12T06:31:09Z |
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| id | doaj.art-85d02d0e80fe448b94450ab8ac3d654c |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T06:31:09Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj.art-85d02d0e80fe448b94450ab8ac3d654c2023-09-03T01:38:20ZengMDPI AGBioengineering2306-53542017-08-01437310.3390/bioengineering4030073bioengineering4030073The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate ProductionBrian Johnston0Guozhan Jiang1David Hill2Grazyna Adamus3Iwona Kwiecień4Magdalena Zięba5Wanda Sikorska6Matthew Green7Marek Kowalczuk8Iza Radecka9Wolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKWolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKWolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-800 Zabrze, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-800 Zabrze, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-800 Zabrze, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-800 Zabrze, PolandRecycling Technologies Ltd., South Marston Industrial Park, Swindon SN3 4WA, UKWolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKWolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UKThere is an increasing demand for bio-based polymers that are developed from recycled materials. The production of biodegradable polymers can include bio-technological (utilizing microorganisms or enzymes) or chemical synthesis procedures. This report demonstrates the corroboration of the molecular structure of polyhydroxyalkanoates (PHAs) obtained by the conversion of waste polyethylene (PE) via non-oxygenated PE wax (N-PEW) as an additional carbon source for a bacterial species. The N-PEW, obtained from a PE pyrolysis reaction, has been found to be a beneficial carbon source for PHA production with Cupriavidus necator H16. The production of the N-PEW is an alternative to oxidized polyethylene wax (O-PEW) (that has been used as a carbon source previously) as it is less time consuming to manufacture and offers fewer industrial applications. A range of molecular structural analytical techniques were performed on the PHAs obtained; which included nuclear magnetic resonance (NMR) and electrospray ionisation tandem mass spectrometry (ESI-MS/MS). Our study showed that the PHA formed from N-PEW contained 3-hydroxybutyrate (HB) with 11 mol% of 3-hydroxyvalerate (HV) units.https://www.mdpi.com/2306-5354/4/3/73polyhydroxyalkanoatesPHAsnon-oxidized PE waxN-PEWCupriavidus necator H16 |
| spellingShingle | Brian Johnston Guozhan Jiang David Hill Grazyna Adamus Iwona Kwiecień Magdalena Zięba Wanda Sikorska Matthew Green Marek Kowalczuk Iza Radecka The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production Bioengineering polyhydroxyalkanoates PHAs non-oxidized PE wax N-PEW Cupriavidus necator H16 |
| title | The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production |
| title_full | The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production |
| title_fullStr | The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production |
| title_full_unstemmed | The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production |
| title_short | The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production |
| title_sort | molecular level characterization of biodegradable polymers originated from polyethylene using non oxygenated polyethylene wax as a carbon source for polyhydroxyalkanoate production |
| topic | polyhydroxyalkanoates PHAs non-oxidized PE wax N-PEW Cupriavidus necator H16 |
| url | https://www.mdpi.com/2306-5354/4/3/73 |
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