Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics
The omics disciplines remain largely distinct sciences due to the necessity of separating molecular classes for different assays. For example, water-soluble and lipid bilayer-bound proteins and metabolites are usually studied separately. Nonetheless, it is at the interface between these sciences whe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/6/1230 |
| _version_ | 1828856653481508864 |
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| author | Michael Overduin Mansoore Esmaili |
| author_facet | Michael Overduin Mansoore Esmaili |
| author_sort | Michael Overduin |
| collection | DOAJ |
| description | The omics disciplines remain largely distinct sciences due to the necessity of separating molecular classes for different assays. For example, water-soluble and lipid bilayer-bound proteins and metabolites are usually studied separately. Nonetheless, it is at the interface between these sciences where biology happens. That is, lipid-interacting proteins typically recognize and transduce signals and regulate the flow of metabolites in the cell. Technologies are emerging to converge the omics. It is now possible to separate intact membrane:protein assemblies (memteins) directly from intact cells or cell membranes. Such complexes mediate complete metabolon, receptor, channel, and transporter functions. The use of poly(styrene-<i>co</i>-maleic acid) (SMA) copolymers has allowed their separation in a single step without any exposure to synthetic detergents or artificial lipids. This is a critical development as these agents typically strip away biological lipids, signals, and metabolites from their physiologically-relevant positions on proteins. The resulting SMA lipid particles (SMALPs) represent native nanodiscs that are suitable for elucidation of structures and interactions that occur in vivo. Compatible tools for resolving the contained memteins include X-ray diffraction (XRD), cryo-electron microscopy (cryoEM), mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. Recent progress shows that memteins are more representative than naked membrane proteins devoid of natural lipid and is driving the development of next generation polymers. |
| first_indexed | 2024-12-13T01:19:42Z |
| format | Article |
| id | doaj.art-85d0671b641344dabe863db353cadedb |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-13T01:19:42Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-85d0671b641344dabe863db353cadedb2022-12-22T00:04:15ZengMDPI AGApplied Sciences2076-34172019-03-0196123010.3390/app9061230app9061230Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and ProteomicsMichael Overduin0Mansoore Esmaili1Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, CanadaDepartment of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, CanadaThe omics disciplines remain largely distinct sciences due to the necessity of separating molecular classes for different assays. For example, water-soluble and lipid bilayer-bound proteins and metabolites are usually studied separately. Nonetheless, it is at the interface between these sciences where biology happens. That is, lipid-interacting proteins typically recognize and transduce signals and regulate the flow of metabolites in the cell. Technologies are emerging to converge the omics. It is now possible to separate intact membrane:protein assemblies (memteins) directly from intact cells or cell membranes. Such complexes mediate complete metabolon, receptor, channel, and transporter functions. The use of poly(styrene-<i>co</i>-maleic acid) (SMA) copolymers has allowed their separation in a single step without any exposure to synthetic detergents or artificial lipids. This is a critical development as these agents typically strip away biological lipids, signals, and metabolites from their physiologically-relevant positions on proteins. The resulting SMA lipid particles (SMALPs) represent native nanodiscs that are suitable for elucidation of structures and interactions that occur in vivo. Compatible tools for resolving the contained memteins include X-ray diffraction (XRD), cryo-electron microscopy (cryoEM), mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. Recent progress shows that memteins are more representative than naked membrane proteins devoid of natural lipid and is driving the development of next generation polymers.https://www.mdpi.com/2076-3417/9/6/1230interactomelipidlipidomicsmembrane structuretransmembrane proteinmemteinmetabolomicsmetabolonproteomenative nanodiscstyrene maleic acidSMALP |
| spellingShingle | Michael Overduin Mansoore Esmaili Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics Applied Sciences interactome lipid lipidomics membrane structure transmembrane protein memtein metabolomics metabolon proteome native nanodisc styrene maleic acid SMALP |
| title | Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics |
| title_full | Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics |
| title_fullStr | Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics |
| title_full_unstemmed | Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics |
| title_short | Native Nanodiscs and the Convergence of Lipidomics, Metabolomics, Interactomics and Proteomics |
| title_sort | native nanodiscs and the convergence of lipidomics metabolomics interactomics and proteomics |
| topic | interactome lipid lipidomics membrane structure transmembrane protein memtein metabolomics metabolon proteome native nanodisc styrene maleic acid SMALP |
| url | https://www.mdpi.com/2076-3417/9/6/1230 |
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