Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China
Abstract Background The Artemisia species are widely distributed around the world, and have found important usage in traditional medicinal practice. This study was designed to investigate the metabolites of Tibetan Artemisia species and understand the metabolic pathways. Methods The metabolites from...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03513-z |
| _version_ | 1818319757516996608 |
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| author | Xinyu Liu Jinglong Wang Enxia Huang Bo Li Shuhang Zhang Weina Wang Ziyu Guo Kexin Wu Yunhao Zhang Baoyu Zhao Hao Lu |
| author_facet | Xinyu Liu Jinglong Wang Enxia Huang Bo Li Shuhang Zhang Weina Wang Ziyu Guo Kexin Wu Yunhao Zhang Baoyu Zhao Hao Lu |
| author_sort | Xinyu Liu |
| collection | DOAJ |
| description | Abstract Background The Artemisia species are widely distributed around the world, and have found important usage in traditional medicinal practice. This study was designed to investigate the metabolites of Tibetan Artemisia species and understand the metabolic pathways. Methods The metabolites from three Artemisia species in Tibet, were analyzed using LC–MS/MS. The differential metabolites were classified and analyzed by principal component analysis (PCA), partial least squares analysis and hierarchical clustering. KEGG Pathway enrichment analysis was used to identify the key metabolic pathways involved in the differential metabolites of three Artemisia species. Result The metabolites of three Artemisia species were analyzed. Under the positive ion mode in LC–MS/MS, 262 distinct metabolites were differentially detected from Artemisia sieversiana and Artemisia annua, 312 differential metabolites were detected from Artemisia wellbyi and Artemisia sieversiana, 306 differential metabolites were screened from Artemisia wellbyi and Artemisia annua. With the negative ion mode, 106 differential metabolites were identified from Artemisia sieversiana and Artemisia annua, 131 differential metabolites were identified from Artemisia wellbyi and Artemisia sieversiana,133 differential metabolites were differentially detected from Artemisia wellbyi and Artemisia annua. The selected differential metabolites were mainly organic acids and their derivatives, ketones, phenols, alcohols and coumarins. Among these natural compounds, artemisinin, has the highest relative content in Artemisia annua. Conclusions This is the first reported attempt to comparatively determine the types of the metabolites of the three widely distributed Artemisia species in Tibet. The information should help medicinal research and facilitate comprehensive development and utilization of Artemisia species in Tibet. |
| first_indexed | 2024-12-13T10:14:11Z |
| format | Article |
| id | doaj.art-85d0e6c71b8640848f0137d50d1aef2d |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-13T10:14:11Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-85d0e6c71b8640848f0137d50d1aef2d2022-12-21T23:51:21ZengBMCBMC Plant Biology1471-22292022-03-0122111410.1186/s12870-022-03513-zMetabolomics analysis of three Artemisia species in the Tibet autonomous region of ChinaXinyu Liu0Jinglong Wang1Enxia Huang2Bo Li3Shuhang Zhang4Weina Wang5Ziyu Guo6Kexin Wu7Yunhao Zhang8Baoyu Zhao9Hao Lu10College of Veterinary Medicine, Northwest A&F UniversityTibet Academy of Agricultural and Animal Husbandry Sciences/State Key Laboratory of Barley and Yak Germplasm Resources and Genetic ImprovementCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityCollege of Veterinary Medicine, Northwest A&F UniversityAbstract Background The Artemisia species are widely distributed around the world, and have found important usage in traditional medicinal practice. This study was designed to investigate the metabolites of Tibetan Artemisia species and understand the metabolic pathways. Methods The metabolites from three Artemisia species in Tibet, were analyzed using LC–MS/MS. The differential metabolites were classified and analyzed by principal component analysis (PCA), partial least squares analysis and hierarchical clustering. KEGG Pathway enrichment analysis was used to identify the key metabolic pathways involved in the differential metabolites of three Artemisia species. Result The metabolites of three Artemisia species were analyzed. Under the positive ion mode in LC–MS/MS, 262 distinct metabolites were differentially detected from Artemisia sieversiana and Artemisia annua, 312 differential metabolites were detected from Artemisia wellbyi and Artemisia sieversiana, 306 differential metabolites were screened from Artemisia wellbyi and Artemisia annua. With the negative ion mode, 106 differential metabolites were identified from Artemisia sieversiana and Artemisia annua, 131 differential metabolites were identified from Artemisia wellbyi and Artemisia sieversiana,133 differential metabolites were differentially detected from Artemisia wellbyi and Artemisia annua. The selected differential metabolites were mainly organic acids and their derivatives, ketones, phenols, alcohols and coumarins. Among these natural compounds, artemisinin, has the highest relative content in Artemisia annua. Conclusions This is the first reported attempt to comparatively determine the types of the metabolites of the three widely distributed Artemisia species in Tibet. The information should help medicinal research and facilitate comprehensive development and utilization of Artemisia species in Tibet.https://doi.org/10.1186/s12870-022-03513-zArtemisia sieversianaArtemisia wellbyiArtemisia annuaNon-targeted metabolomicsLC–MS/MSTibet |
| spellingShingle | Xinyu Liu Jinglong Wang Enxia Huang Bo Li Shuhang Zhang Weina Wang Ziyu Guo Kexin Wu Yunhao Zhang Baoyu Zhao Hao Lu Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China BMC Plant Biology Artemisia sieversiana Artemisia wellbyi Artemisia annua Non-targeted metabolomics LC–MS/MS Tibet |
| title | Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China |
| title_full | Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China |
| title_fullStr | Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China |
| title_full_unstemmed | Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China |
| title_short | Metabolomics analysis of three Artemisia species in the Tibet autonomous region of China |
| title_sort | metabolomics analysis of three artemisia species in the tibet autonomous region of china |
| topic | Artemisia sieversiana Artemisia wellbyi Artemisia annua Non-targeted metabolomics LC–MS/MS Tibet |
| url | https://doi.org/10.1186/s12870-022-03513-z |
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