Principles and medical applications of gene editing by CRISPR / Cas
CRISPR/Cas technology is a simple, fast and extremely efficient method for gene editing. In order to describe the principles and medical application of this technology, a bibliographic review was carried out in Pubmed, SciELO, academic Google and the Cochrane Library, with the descriptors “gene edit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Spanish |
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Centro Provincial de Información de Ciencias Médicas. Cienfuegos
2021-12-01
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| Series: | Medisur |
| Subjects: | |
| Online Access: | http://medisur.sld.cu/index.php/medisur/article/view/5279 |
| _version_ | 1797277719369613312 |
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| author | Rolando Sánchez Artigas María Teresa Díaz Armas Raisa Rodríguez Duque Pedro Enrique Miguel Soca |
| author_facet | Rolando Sánchez Artigas María Teresa Díaz Armas Raisa Rodríguez Duque Pedro Enrique Miguel Soca |
| author_sort | Rolando Sánchez Artigas |
| collection | DOAJ |
| description | CRISPR/Cas technology is a simple, fast and extremely efficient method for gene editing. In order to describe the principles and medical application of this technology, a bibliographic review was carried out in Pubmed, SciELO, academic Google and the Cochrane Library, with the descriptors “gene editing”, “genome editing”, “CRISPR-Cas systems”, and “CRISPR-associated protein 9”. The CRISPR/Cas9 system comprises a Cas endonuclease and two types of RNA. Cas cuts the invading phage DNA into segments, which are integrated into the CRISPR sequence as spacers. Subsequently, the CRISPR sequence is transcribed to generate crRNA and tracrRNA, which form a double-stranded RNA structure that recruits Cas for cleavage. The introduction of the system into the cell interior occurs with plasmids, RNA or ribonucleoproteins. A nuclear localization sequence allows CRISPR / Cas9 to enter the nucleus. CRISPR/Cas9 technology is an efficient tool for precise gene editing with great impact on scientific research. |
| first_indexed | 2024-03-07T15:53:21Z |
| format | Article |
| id | doaj.art-85d213e5431648f3b5bc216ecaa9d846 |
| institution | Directory Open Access Journal |
| issn | 1727-897X |
| language | Spanish |
| last_indexed | 2024-03-07T15:53:21Z |
| publishDate | 2021-12-01 |
| publisher | Centro Provincial de Información de Ciencias Médicas. Cienfuegos |
| record_format | Article |
| series | Medisur |
| spelling | doaj.art-85d213e5431648f3b5bc216ecaa9d8462024-03-04T23:09:52ZspaCentro Provincial de Información de Ciencias Médicas. CienfuegosMedisur1727-897X2021-12-01196100510142076Principles and medical applications of gene editing by CRISPR / CasRolando Sánchez Artigas0María Teresa Díaz Armas1Raisa Rodríguez Duque2Pedro Enrique Miguel Soca3Universidad San Gregorio de Portoviejo. PortoviejoEscuela Superior Politécnica de Chimborazo. ChimborazoEscuela Superior Politécnica de Chimborazo. ChimborazoUniversidad de Ciencias Médicas de Holguín. HolguínCRISPR/Cas technology is a simple, fast and extremely efficient method for gene editing. In order to describe the principles and medical application of this technology, a bibliographic review was carried out in Pubmed, SciELO, academic Google and the Cochrane Library, with the descriptors “gene editing”, “genome editing”, “CRISPR-Cas systems”, and “CRISPR-associated protein 9”. The CRISPR/Cas9 system comprises a Cas endonuclease and two types of RNA. Cas cuts the invading phage DNA into segments, which are integrated into the CRISPR sequence as spacers. Subsequently, the CRISPR sequence is transcribed to generate crRNA and tracrRNA, which form a double-stranded RNA structure that recruits Cas for cleavage. The introduction of the system into the cell interior occurs with plasmids, RNA or ribonucleoproteins. A nuclear localization sequence allows CRISPR / Cas9 to enter the nucleus. CRISPR/Cas9 technology is an efficient tool for precise gene editing with great impact on scientific research.http://medisur.sld.cu/index.php/medisur/article/view/5279edición génicasistemas crispr-casproteína 9 asociada a crispr |
| spellingShingle | Rolando Sánchez Artigas María Teresa Díaz Armas Raisa Rodríguez Duque Pedro Enrique Miguel Soca Principles and medical applications of gene editing by CRISPR / Cas Medisur edición génica sistemas crispr-cas proteína 9 asociada a crispr |
| title | Principles and medical applications of gene editing by CRISPR / Cas |
| title_full | Principles and medical applications of gene editing by CRISPR / Cas |
| title_fullStr | Principles and medical applications of gene editing by CRISPR / Cas |
| title_full_unstemmed | Principles and medical applications of gene editing by CRISPR / Cas |
| title_short | Principles and medical applications of gene editing by CRISPR / Cas |
| title_sort | principles and medical applications of gene editing by crispr cas |
| topic | edición génica sistemas crispr-cas proteína 9 asociada a crispr |
| url | http://medisur.sld.cu/index.php/medisur/article/view/5279 |
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