High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence
Protein lysine lactylation (Kla) is a novel protein acylation reported in recent years, which plays an important role in the development of several diseases with pathologically elevated lactate levels, such as tumors. The concentration of lactate as a donor is directly related to the Kla level. High...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2218-1989/13/5/647 |
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| author | Wenhua Huang Jie Su Xuefei Chen Yanjun Li Zheng Xing Lanlan Guo Shitian Li Jing Zhang |
| author_facet | Wenhua Huang Jie Su Xuefei Chen Yanjun Li Zheng Xing Lanlan Guo Shitian Li Jing Zhang |
| author_sort | Wenhua Huang |
| collection | DOAJ |
| description | Protein lysine lactylation (Kla) is a novel protein acylation reported in recent years, which plays an important role in the development of several diseases with pathologically elevated lactate levels, such as tumors. The concentration of lactate as a donor is directly related to the Kla level. High-intensity interval training (HIIT) is a workout pattern that has positive effects in many metabolic diseases, but the mechanisms by which HIIT promotes health are not yet clear. Lactate is the main metabolite of HIIT, and it is unknown as to whether high lactate during HIIT can induce changes in Kla levels, as well as whether Kla levels differ in different tissues and how time-dependent Kla levels are. In this study, we observed the specificity and time-dependent effects of a single HIIT on the regulation of Kla in mouse tissues. In addition, we aimed to select tissues with high Kla specificity and obvious time dependence for lactylation quantitative omics and analyze the possible biological targets of HIIT-induced Kla regulation. A single HIIT induces Kla in tissues with high lactate uptake and metabolism, such as iWAT, BAT, soleus muscle and liver proteins, and Kla levels peak at 24 h after HIIT and return to steady state at 72 h. Kla proteins in iWAT may affect pathways related to glycolipid metabolism and are highly associated with de novo synthesis. It is speculated that the changes in energy expenditure, lipolytic effects and metabolic characteristics during the recovery period after HIIT may be related to the regulation of Kla in iWAT. |
| first_indexed | 2024-03-11T03:30:28Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T03:30:28Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-85d2bf7cd5da45ad8c295b2053d566ca2023-11-18T02:25:50ZengMDPI AGMetabolites2218-19892023-05-0113564710.3390/metabo13050647High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time DependenceWenhua Huang0Jie Su1Xuefei Chen2Yanjun Li3Zheng Xing4Lanlan Guo5Shitian Li6Jing Zhang7School of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaSchool of P.E. and Sports Science, Beijing Normal University, Beijing 100875, ChinaProtein lysine lactylation (Kla) is a novel protein acylation reported in recent years, which plays an important role in the development of several diseases with pathologically elevated lactate levels, such as tumors. The concentration of lactate as a donor is directly related to the Kla level. High-intensity interval training (HIIT) is a workout pattern that has positive effects in many metabolic diseases, but the mechanisms by which HIIT promotes health are not yet clear. Lactate is the main metabolite of HIIT, and it is unknown as to whether high lactate during HIIT can induce changes in Kla levels, as well as whether Kla levels differ in different tissues and how time-dependent Kla levels are. In this study, we observed the specificity and time-dependent effects of a single HIIT on the regulation of Kla in mouse tissues. In addition, we aimed to select tissues with high Kla specificity and obvious time dependence for lactylation quantitative omics and analyze the possible biological targets of HIIT-induced Kla regulation. A single HIIT induces Kla in tissues with high lactate uptake and metabolism, such as iWAT, BAT, soleus muscle and liver proteins, and Kla levels peak at 24 h after HIIT and return to steady state at 72 h. Kla proteins in iWAT may affect pathways related to glycolipid metabolism and are highly associated with de novo synthesis. It is speculated that the changes in energy expenditure, lipolytic effects and metabolic characteristics during the recovery period after HIIT may be related to the regulation of Kla in iWAT.https://www.mdpi.com/2218-1989/13/5/647high-intensity interval traininglysine lactylationenergy metabolismproteomics |
| spellingShingle | Wenhua Huang Jie Su Xuefei Chen Yanjun Li Zheng Xing Lanlan Guo Shitian Li Jing Zhang High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence Metabolites high-intensity interval training lysine lactylation energy metabolism proteomics |
| title | High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence |
| title_full | High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence |
| title_fullStr | High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence |
| title_full_unstemmed | High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence |
| title_short | High-Intensity Interval Training Induces Protein Lactylation in Different Tissues of Mice with Specificity and Time Dependence |
| title_sort | high intensity interval training induces protein lactylation in different tissues of mice with specificity and time dependence |
| topic | high-intensity interval training lysine lactylation energy metabolism proteomics |
| url | https://www.mdpi.com/2218-1989/13/5/647 |
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