Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study.
Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated by cytokines. Vaginal microbiota determines the presence of certain cytokines locally. We assessed the association between cervical microbiota diversity and the h...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4846060?pdf=render |
| _version_ | 1819051956968095744 |
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| author | Astride Audirac-Chalifour Kirvis Torres-Poveda Margarita Bahena-Román Juan Téllez-Sosa Jesús Martínez-Barnetche Bernardo Cortina-Ceballos Guillermina López-Estrada Karina Delgado-Romero Ana I Burguete-García David Cantú Alejandro García-Carrancá Vicente Madrid-Marina |
| author_facet | Astride Audirac-Chalifour Kirvis Torres-Poveda Margarita Bahena-Román Juan Téllez-Sosa Jesús Martínez-Barnetche Bernardo Cortina-Ceballos Guillermina López-Estrada Karina Delgado-Romero Ana I Burguete-García David Cantú Alejandro García-Carrancá Vicente Madrid-Marina |
| author_sort | Astride Audirac-Chalifour |
| collection | DOAJ |
| description | Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated by cytokines. Vaginal microbiota determines the presence of certain cytokines locally. We assessed the association between cervical microbiota diversity and the histopathological diagnosis of each stage of CC, and we evaluated mRNA cervical expression levels of IL-4, IL-6, IL-10, TGF-β1, TNF-α and IFN-γ across the histopathological diagnosis and specific bacterial clusters. We determined the cervical microbiota by high throughput sequencing of 16S rDNA amplicons and classified it in community state types (CST). Mean difference analyses between alpha-diversity and histopathological diagnosis were carried out, as well as a β-diversity analysis within the histological diagnosis. Cervical cytokine mRNA expression was analyzed across the CSTs and the histopathological diagnoses. We found a significant difference in microbiota's diversity in NCL-HPV negative women vs those with squamous intraepithelial lesions (SIL) and CC(p = 0.006, p = 0.036).When β-diversity was evaluated, the CC samples showed the highest variation within groups (p<0.0006) and the largest distance compared to NCL-HPV negative ones (p<0.00001). The predominant bacteria in women with normal cytology were L. crispatus and L. iners, whereas for SIL, it was Sneathia spp. and for CC, Fusobacterium spp. We found higher median cervical levels of IL-4 and TGF-β1 mRNA in the CST dominated by Fusobacterium spp. These results suggest that the cervical microbiota may be implicated in cervical cancer pathology. Further cohort studies are needed to validate these findings. |
| first_indexed | 2024-12-21T12:12:11Z |
| format | Article |
| id | doaj.art-85d785b7c76449c0a074625fea7779a8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T12:12:11Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-85d785b7c76449c0a074625fea7779a82022-12-21T19:04:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015327410.1371/journal.pone.0153274Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study.Astride Audirac-ChalifourKirvis Torres-PovedaMargarita Bahena-RománJuan Téllez-SosaJesús Martínez-BarnetcheBernardo Cortina-CeballosGuillermina López-EstradaKarina Delgado-RomeroAna I Burguete-GarcíaDavid CantúAlejandro García-CarrancáVicente Madrid-MarinaCervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated by cytokines. Vaginal microbiota determines the presence of certain cytokines locally. We assessed the association between cervical microbiota diversity and the histopathological diagnosis of each stage of CC, and we evaluated mRNA cervical expression levels of IL-4, IL-6, IL-10, TGF-β1, TNF-α and IFN-γ across the histopathological diagnosis and specific bacterial clusters. We determined the cervical microbiota by high throughput sequencing of 16S rDNA amplicons and classified it in community state types (CST). Mean difference analyses between alpha-diversity and histopathological diagnosis were carried out, as well as a β-diversity analysis within the histological diagnosis. Cervical cytokine mRNA expression was analyzed across the CSTs and the histopathological diagnoses. We found a significant difference in microbiota's diversity in NCL-HPV negative women vs those with squamous intraepithelial lesions (SIL) and CC(p = 0.006, p = 0.036).When β-diversity was evaluated, the CC samples showed the highest variation within groups (p<0.0006) and the largest distance compared to NCL-HPV negative ones (p<0.00001). The predominant bacteria in women with normal cytology were L. crispatus and L. iners, whereas for SIL, it was Sneathia spp. and for CC, Fusobacterium spp. We found higher median cervical levels of IL-4 and TGF-β1 mRNA in the CST dominated by Fusobacterium spp. These results suggest that the cervical microbiota may be implicated in cervical cancer pathology. Further cohort studies are needed to validate these findings.http://europepmc.org/articles/PMC4846060?pdf=render |
| spellingShingle | Astride Audirac-Chalifour Kirvis Torres-Poveda Margarita Bahena-Román Juan Téllez-Sosa Jesús Martínez-Barnetche Bernardo Cortina-Ceballos Guillermina López-Estrada Karina Delgado-Romero Ana I Burguete-García David Cantú Alejandro García-Carrancá Vicente Madrid-Marina Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. PLoS ONE |
| title | Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. |
| title_full | Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. |
| title_fullStr | Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. |
| title_full_unstemmed | Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. |
| title_short | Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study. |
| title_sort | cervical microbiome and cytokine profile at various stages of cervical cancer a pilot study |
| url | http://europepmc.org/articles/PMC4846060?pdf=render |
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