First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria
The aim of this study was to assess the diversity of minisatellite VNTR loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> isolates in Bulgaria and view their position within global <i>M. bovis</i> diversity. Forty-three <i>M. bovis</i>/<i>M. ca...
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MDPI AG
2023-02-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/4/771 |
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| author | Daria Terentieva Tanya Savova-Lalkovska Albena Dimitrova Magdalena Bonovska Igor Mokrousov Violeta Valcheva |
| author_facet | Daria Terentieva Tanya Savova-Lalkovska Albena Dimitrova Magdalena Bonovska Igor Mokrousov Violeta Valcheva |
| author_sort | Daria Terentieva |
| collection | DOAJ |
| description | The aim of this study was to assess the diversity of minisatellite VNTR loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> isolates in Bulgaria and view their position within global <i>M. bovis</i> diversity. Forty-three <i>M. bovis</i>/<i>M. caprae</i> isolates from cattle in different farms in Bulgaria were collected in 2015–2021 and typed in 13 VNTR loci. The <i>M. bovis</i> and <i>M. caprae</i> branches were clearly separated on the VNTR phylogenetic tree. The larger and more geographically dispersed <i>M. caprae</i> group was more diverse than <i>M. bovis</i> group was (HGI 0.67 vs. 0.60). Overall, six clusters were identified (from 2 to 19 isolates) and nine orphans (all loci-based HGI 0.79). Locus QUB3232 was the most discriminatory one (HGI 0.64). MIRU4 and MIRU40 were monomorphic, and MIRU26 was almost monomorphic. Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between <i>M. bovis</i> and <i>M. caprae</i>. The comparison with published VNTR datasets from 11 countries showed both overall heterogeneity between the settings and predominantly local evolution of the clonal complexes. To conclude, six loci may be recommended for primary genotyping of <i>M. bovis</i>/<i>M. caprae</i> isolates in Bulgaria: ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 (HGI 0.77). VNTR typing based on a limited number of loci appears to be useful for primary bTB surveillance. |
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| format | Article |
| id | doaj.art-85d8637ae7754dc69c2efd259e374291 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T08:57:03Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-85d8637ae7754dc69c2efd259e3742912023-11-16T20:02:45ZengMDPI AGDiagnostics2075-44182023-02-0113477110.3390/diagnostics13040771First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in BulgariaDaria Terentieva0Tanya Savova-Lalkovska1Albena Dimitrova2Magdalena Bonovska3Igor Mokrousov4Violeta Valcheva5Laboratory of Molecular Epidemiology and Evolutionary Genetics, St. Petersburg Pasteur Institute, 197101 St. Petersburg, RussiaNational Reference Laboratory for Bovine Tuberculosis, National Diagnostic and Research Veterinary Medical Institute “Prof. Dr. G. Pavlov”, 1606 Sofia, BulgariaNational Reference Laboratory for Bovine Tuberculosis, National Diagnostic and Research Veterinary Medical Institute “Prof. Dr. G. Pavlov”, 1606 Sofia, BulgariaDepartment of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaLaboratory of Molecular Epidemiology and Evolutionary Genetics, St. Petersburg Pasteur Institute, 197101 St. Petersburg, RussiaDepartment of Infectious Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaThe aim of this study was to assess the diversity of minisatellite VNTR loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> isolates in Bulgaria and view their position within global <i>M. bovis</i> diversity. Forty-three <i>M. bovis</i>/<i>M. caprae</i> isolates from cattle in different farms in Bulgaria were collected in 2015–2021 and typed in 13 VNTR loci. The <i>M. bovis</i> and <i>M. caprae</i> branches were clearly separated on the VNTR phylogenetic tree. The larger and more geographically dispersed <i>M. caprae</i> group was more diverse than <i>M. bovis</i> group was (HGI 0.67 vs. 0.60). Overall, six clusters were identified (from 2 to 19 isolates) and nine orphans (all loci-based HGI 0.79). Locus QUB3232 was the most discriminatory one (HGI 0.64). MIRU4 and MIRU40 were monomorphic, and MIRU26 was almost monomorphic. Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between <i>M. bovis</i> and <i>M. caprae</i>. The comparison with published VNTR datasets from 11 countries showed both overall heterogeneity between the settings and predominantly local evolution of the clonal complexes. To conclude, six loci may be recommended for primary genotyping of <i>M. bovis</i>/<i>M. caprae</i> isolates in Bulgaria: ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 (HGI 0.77). VNTR typing based on a limited number of loci appears to be useful for primary bTB surveillance.https://www.mdpi.com/2075-4418/13/4/771<i>Mycobacterium bovis</i><i>Mycobacterium caprae</i>VNTRgenotypingwhole-genome sequencingBulgaria |
| spellingShingle | Daria Terentieva Tanya Savova-Lalkovska Albena Dimitrova Magdalena Bonovska Igor Mokrousov Violeta Valcheva First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria Diagnostics <i>Mycobacterium bovis</i> <i>Mycobacterium caprae</i> VNTR genotyping whole-genome sequencing Bulgaria |
| title | First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria |
| title_full | First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria |
| title_fullStr | First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria |
| title_full_unstemmed | First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria |
| title_short | First Insight into Diversity of Minisatellite Loci in <i>Mycobacterium bovis</i>/<i>M. caprae</i> in Bulgaria |
| title_sort | first insight into diversity of minisatellite loci in i mycobacterium bovis i i m caprae i in bulgaria |
| topic | <i>Mycobacterium bovis</i> <i>Mycobacterium caprae</i> VNTR genotyping whole-genome sequencing Bulgaria |
| url | https://www.mdpi.com/2075-4418/13/4/771 |
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