Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers
Abstract A novel approach termed the "concentrated method" was developed for the instant fabrication of laccase@Co3(PO4)2•hybrid nanoflowers (HNFs). The constructed HNFs were obtained by optimizing the concentration of cobalt chloride and phosphate buffer to reach the highest activity reco...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13490-w |
| _version_ | 1818553364481310720 |
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| author | Khashayar Vojdanitalab Hossein Jafari-Nodoushan Somayeh Mojtabavi Mahtab Shokri Hoda Jahandar Mohammad Ali Faramarzi |
| author_facet | Khashayar Vojdanitalab Hossein Jafari-Nodoushan Somayeh Mojtabavi Mahtab Shokri Hoda Jahandar Mohammad Ali Faramarzi |
| author_sort | Khashayar Vojdanitalab |
| collection | DOAJ |
| description | Abstract A novel approach termed the "concentrated method" was developed for the instant fabrication of laccase@Co3(PO4)2•hybrid nanoflowers (HNFs). The constructed HNFs were obtained by optimizing the concentration of cobalt chloride and phosphate buffer to reach the highest activity recovery. The incorporation of 30 mM CoCl2 and 160 mM phosphate buffer (pH 7.4) resulted in a fast anisotropic growth of the nanomaterials. The purposed method did not involve harsh conditions and prolonged incubation of precursors, as the most reported approaches for the synthesis of HNFs. The catalytic efficiency of the immobilized and free laccase was 460 and 400 M−1S−1, respectively. Also, the enzymatic activity of the prepared biocatalyst was 113% of the free enzyme (0.5 U mL−1). The stability of the synthesized HNFs was enhanced by 400% at pH 6.5–9.5 and the elevated temperatures. The activity of laccase@Co3(PO4)2•HNFs declined to 50% of the initial value after 10 reusability cycles, indicating successful immobilization of the enzyme. Structural studies revealed a 32% increase in the α-helix content after hybridization with cobalt phosphate, which improved the activity and stability of the immobilized laccase. Furthermore, the fabricated HNFs exhibited a considerable ability to remove moxifloxacin as an emerging pollutant. The antibiotic (10 mg L−1) was removed by 24% and 75% after 24 h through adsorption and biodegradation, respectively. This study introduces a new method for synthesizing HNFs, which could be used for the fabrication of efficient biocatalysts, biosensors, and adsorbents for industrial, biomedical, and environmental applications. |
| first_indexed | 2024-12-12T09:24:53Z |
| format | Article |
| id | doaj.art-85d8956dadb64fd2850bb3b3a8f697a4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T09:24:53Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-85d8956dadb64fd2850bb3b3a8f697a42022-12-22T00:29:03ZengNature PortfolioScientific Reports2045-23222022-06-0112111610.1038/s41598-022-13490-wInstantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowersKhashayar Vojdanitalab0Hossein Jafari-Nodoushan1Somayeh Mojtabavi2Mahtab Shokri3Hoda Jahandar4Mohammad Ali Faramarzi5Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical SciencesDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical SciencesDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical SciencesDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical SciencesPharmaceutical Sciences Research Center, Tehran Medical Sciences Branch, Islamic Azad UniversityDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical SciencesAbstract A novel approach termed the "concentrated method" was developed for the instant fabrication of laccase@Co3(PO4)2•hybrid nanoflowers (HNFs). The constructed HNFs were obtained by optimizing the concentration of cobalt chloride and phosphate buffer to reach the highest activity recovery. The incorporation of 30 mM CoCl2 and 160 mM phosphate buffer (pH 7.4) resulted in a fast anisotropic growth of the nanomaterials. The purposed method did not involve harsh conditions and prolonged incubation of precursors, as the most reported approaches for the synthesis of HNFs. The catalytic efficiency of the immobilized and free laccase was 460 and 400 M−1S−1, respectively. Also, the enzymatic activity of the prepared biocatalyst was 113% of the free enzyme (0.5 U mL−1). The stability of the synthesized HNFs was enhanced by 400% at pH 6.5–9.5 and the elevated temperatures. The activity of laccase@Co3(PO4)2•HNFs declined to 50% of the initial value after 10 reusability cycles, indicating successful immobilization of the enzyme. Structural studies revealed a 32% increase in the α-helix content after hybridization with cobalt phosphate, which improved the activity and stability of the immobilized laccase. Furthermore, the fabricated HNFs exhibited a considerable ability to remove moxifloxacin as an emerging pollutant. The antibiotic (10 mg L−1) was removed by 24% and 75% after 24 h through adsorption and biodegradation, respectively. This study introduces a new method for synthesizing HNFs, which could be used for the fabrication of efficient biocatalysts, biosensors, and adsorbents for industrial, biomedical, and environmental applications.https://doi.org/10.1038/s41598-022-13490-w |
| spellingShingle | Khashayar Vojdanitalab Hossein Jafari-Nodoushan Somayeh Mojtabavi Mahtab Shokri Hoda Jahandar Mohammad Ali Faramarzi Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers Scientific Reports |
| title | Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers |
| title_full | Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers |
| title_fullStr | Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers |
| title_full_unstemmed | Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers |
| title_short | Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers |
| title_sort | instantaneous synthesis and full characterization of organic inorganic laccase cobalt phosphate hybrid nanoflowers |
| url | https://doi.org/10.1038/s41598-022-13490-w |
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