The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection
Plant pathogens secrete small proteins, of which some are effectors that promote infection. During colonization of the tomato xylem vessels the fungus Fusarium oxysporum f. sp. lycopersici (Fol) secretes small proteins that are referred to as SIX (Secreted In Xylem) proteins. Of these, Six1 (Avr3),...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00967/full |
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| author | Fleur eGawehns Fleur eGawehns Lisong eMa Lisong eMa Oskar eBruning Petra M Houterman Sjef eBoeren Ben J.C. Cornelissen Martijn eRep Frank L.W. Takken |
| author_facet | Fleur eGawehns Fleur eGawehns Lisong eMa Lisong eMa Oskar eBruning Petra M Houterman Sjef eBoeren Ben J.C. Cornelissen Martijn eRep Frank L.W. Takken |
| author_sort | Fleur eGawehns |
| collection | DOAJ |
| description | Plant pathogens secrete small proteins, of which some are effectors that promote infection. During colonization of the tomato xylem vessels the fungus Fusarium oxysporum f. sp. lycopersici (Fol) secretes small proteins that are referred to as SIX (Secreted In Xylem) proteins. Of these, Six1 (Avr3), Six3 (Avr2), Six5 and Six6 are required for full virulence, denoting them as effectors. To investigate their activities in the plant, the xylem sap proteome of plants inoculated with Fol wild-type or either AVR2, AVR3, SIX2, SIX5 or SIX6 knockout strains was analyzed with nano-Liquid Chromatography-Mass Spectrometry (nLC-MSMS). Compared to mock-inoculated sap 12 additional plant proteins appeared while 45 proteins were no longer detectable in the xylem sap of Fol-infected plants. Of the 285 proteins found in both uninfected and infected plants the abundance of 258 proteins changed significantly following infection. The xylem sap proteome of plants infected with four Fol effector knockout strains differed significantly from plants infected with wild-type Fol, while that of the SIX2-knockout inoculated plants remained unchanged. Besides an altered abundance of a core set of 24 differentially accumulated proteins (DAPs), each of the four effector knockout strains affected specifically the abundance of a subset of DAPs. Hence, Fol effectors have both unique and shared effects on the composition of the tomato xylem sap proteome. |
| first_indexed | 2024-04-13T11:09:02Z |
| format | Article |
| id | doaj.art-85db9de182434d099d6e0e601dc481ab |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T11:09:02Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-85db9de182434d099d6e0e601dc481ab2022-12-22T02:49:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-11-01610.3389/fpls.2015.00967165377The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infectionFleur eGawehns0Fleur eGawehns1Lisong eMa2Lisong eMa3Oskar eBruning4Petra M Houterman5Sjef eBoeren6Ben J.C. Cornelissen7Martijn eRep8Frank L.W. Takken9University of AmsterdamNaktuinbouwUniversity of AmsterdamSaskatoon Research CentreFaculty of Science, University of Amsterdam University of AmsterdamWageningen UniversityUniversity of AmsterdamUniversity of AmsterdamUniversity of AmsterdamPlant pathogens secrete small proteins, of which some are effectors that promote infection. During colonization of the tomato xylem vessels the fungus Fusarium oxysporum f. sp. lycopersici (Fol) secretes small proteins that are referred to as SIX (Secreted In Xylem) proteins. Of these, Six1 (Avr3), Six3 (Avr2), Six5 and Six6 are required for full virulence, denoting them as effectors. To investigate their activities in the plant, the xylem sap proteome of plants inoculated with Fol wild-type or either AVR2, AVR3, SIX2, SIX5 or SIX6 knockout strains was analyzed with nano-Liquid Chromatography-Mass Spectrometry (nLC-MSMS). Compared to mock-inoculated sap 12 additional plant proteins appeared while 45 proteins were no longer detectable in the xylem sap of Fol-infected plants. Of the 285 proteins found in both uninfected and infected plants the abundance of 258 proteins changed significantly following infection. The xylem sap proteome of plants infected with four Fol effector knockout strains differed significantly from plants infected with wild-type Fol, while that of the SIX2-knockout inoculated plants remained unchanged. Besides an altered abundance of a core set of 24 differentially accumulated proteins (DAPs), each of the four effector knockout strains affected specifically the abundance of a subset of DAPs. Hence, Fol effectors have both unique and shared effects on the composition of the tomato xylem sap proteome.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00967/fullVirulenceEffectorspathogenicityxylem saplabel free proteomics |
| spellingShingle | Fleur eGawehns Fleur eGawehns Lisong eMa Lisong eMa Oskar eBruning Petra M Houterman Sjef eBoeren Ben J.C. Cornelissen Martijn eRep Frank L.W. Takken The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection Frontiers in Plant Science Virulence Effectors pathogenicity xylem sap label free proteomics |
| title | The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection |
| title_full | The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection |
| title_fullStr | The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection |
| title_full_unstemmed | The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection |
| title_short | The effector repertoire of Fusarium oxysporum determines the tomato xylem proteome composition following infection |
| title_sort | effector repertoire of fusarium oxysporum determines the tomato xylem proteome composition following infection |
| topic | Virulence Effectors pathogenicity xylem sap label free proteomics |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00967/full |
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