Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects
Pregnant women must be wary of using traditional medicines due to the possibility of their having oxytoxic effects. Indonesia is rich in plants containing antioxidants. One of these plants is <i>Phyllanthus emblica</i> L. This study aims to determine the phytochemical constituents of <...
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MDPI AG
2024-04-01
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| Series: | Molecules |
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| author | Masfria Aminah Dalimunthe Nurul Suci Hafid Syahputra |
| author_facet | Masfria Aminah Dalimunthe Nurul Suci Hafid Syahputra |
| author_sort | Masfria |
| collection | DOAJ |
| description | Pregnant women must be wary of using traditional medicines due to the possibility of their having oxytoxic effects. Indonesia is rich in plants containing antioxidants. One of these plants is <i>Phyllanthus emblica</i> L. This study aims to determine the phytochemical constituents of <i>Phyllanthus emblica</i> L. fruit nanoherbals by LC-HRMS analysis and their antimutagenic activity and teratogenic effects. The study commenced by producing nanoherbal extracts from <i>P. emblica</i> fruit. The phytochemical composition of these extracts was then analyzed using LC-HRMS. The nanoherbal extracts were also tested for their ability to prevent mutations, as indicated by a reduction in micronuclei observed in mouse femur bone marrow smear preparations. The teratogenicity test involved administering the <i>P. emblica</i> fruit nanoherbal at 100, 500, and 1000 mg/kg BW doses. The data were analyzed using SPSS. The phytochemical constituents of the <i>P. emblica</i> fruit nanoherbal include flavonoids, phenols, vitamins, and alkaloids. The <i>P. emblica</i> fruit nanoherbal exhibits antimutagenic activity, as evidenced by a statistical analysis that indicated a significant decrease in the quantity of micronuclei per 200 PCE compared to the negative control (<i>p</i> < 0.05). The administration of the <i>P. emblica</i> fruit nanoherbal at a dosage of 1000 mg/kg BW resulted in a teratogenic impact during the organogenesis stage, as shown by hemorrhage and anomalies in the sternum. |
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| last_indexed | 2024-04-24T10:38:02Z |
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| spelling | doaj.art-85dbdfe2e9a94b20b29927e64e1f2ebe2024-04-12T13:23:39ZengMDPI AGMolecules1420-30492024-04-01297164210.3390/molecules29071642Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic EffectsMasfria0Aminah Dalimunthe1Nurul Suci2Hafid Syahputra3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, IndonesiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, IndonesiaPregnant women must be wary of using traditional medicines due to the possibility of their having oxytoxic effects. Indonesia is rich in plants containing antioxidants. One of these plants is <i>Phyllanthus emblica</i> L. This study aims to determine the phytochemical constituents of <i>Phyllanthus emblica</i> L. fruit nanoherbals by LC-HRMS analysis and their antimutagenic activity and teratogenic effects. The study commenced by producing nanoherbal extracts from <i>P. emblica</i> fruit. The phytochemical composition of these extracts was then analyzed using LC-HRMS. The nanoherbal extracts were also tested for their ability to prevent mutations, as indicated by a reduction in micronuclei observed in mouse femur bone marrow smear preparations. The teratogenicity test involved administering the <i>P. emblica</i> fruit nanoherbal at 100, 500, and 1000 mg/kg BW doses. The data were analyzed using SPSS. The phytochemical constituents of the <i>P. emblica</i> fruit nanoherbal include flavonoids, phenols, vitamins, and alkaloids. The <i>P. emblica</i> fruit nanoherbal exhibits antimutagenic activity, as evidenced by a statistical analysis that indicated a significant decrease in the quantity of micronuclei per 200 PCE compared to the negative control (<i>p</i> < 0.05). The administration of the <i>P. emblica</i> fruit nanoherbal at a dosage of 1000 mg/kg BW resulted in a teratogenic impact during the organogenesis stage, as shown by hemorrhage and anomalies in the sternum.https://www.mdpi.com/1420-3049/29/7/1642<i>Phyllanthus emblica</i> L. fruit nanoherbalantimutagenicteratogenicLC-HRMS |
| spellingShingle | Masfria Aminah Dalimunthe Nurul Suci Hafid Syahputra Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects Molecules <i>Phyllanthus emblica</i> L. fruit nanoherbal antimutagenic teratogenic LC-HRMS |
| title | Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects |
| title_full | Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects |
| title_fullStr | Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects |
| title_full_unstemmed | Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects |
| title_short | Phytochemical Constituent Analysis of <i>Phyllanthus emblica</i> L. Fruit Nanoherbals by LC-HRMS and Their Antimutagenic Activity and Teratogenic Effects |
| title_sort | phytochemical constituent analysis of i phyllanthus emblica i l fruit nanoherbals by lc hrms and their antimutagenic activity and teratogenic effects |
| topic | <i>Phyllanthus emblica</i> L. fruit nanoherbal antimutagenic teratogenic LC-HRMS |
| url | https://www.mdpi.com/1420-3049/29/7/1642 |
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