Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics
Context Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear.Objective To investigate the antiperspirant effect and underlying mechanisms of MLS.Materials and methods Fifty rats were divided into control, model, and th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2022.2074465 |
| _version_ | 1811253496882659328 |
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| author | Shan-Peng Ma Wei-Ping Ma Shi-Ning Yin Xiang-Yue Chen Xiao-Qing Ma Bao-Hong Wei Jing-Guang Lu Hong-Bing Liu |
| author_facet | Shan-Peng Ma Wei-Ping Ma Shi-Ning Yin Xiang-Yue Chen Xiao-Qing Ma Bao-Hong Wei Jing-Guang Lu Hong-Bing Liu |
| author_sort | Shan-Peng Ma |
| collection | DOAJ |
| description | Context Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear.Objective To investigate the antiperspirant effect and underlying mechanisms of MLS.Materials and methods Fifty rats were divided into control, model, and three doses of MLS intervention groups (n = 10). Rats except for control group were induced diseases features of the applicable scope of MLS via i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats via s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-γ, and TNF-α. Rat plasma was collected for endogenous metabolite analysis via UPLC-QE-Focus-MS.Results Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio via increasing IL-2 (38.3%), IFN-γ (20.1%), and TNF-α (22.0%) and decreasing IL-6 (24.7%) compared with the model group (p < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS (p < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism.Conclusions MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect. |
| first_indexed | 2024-04-12T16:51:55Z |
| format | Article |
| id | doaj.art-85ddfcbd276443949f5800bbb2562462 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-12T16:51:55Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-85ddfcbd276443949f5800bbb25624622022-12-22T03:24:23ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162022-12-016011055106210.1080/13880209.2022.2074465Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomicsShan-Peng Ma0Wei-Ping Ma1Shi-Ning Yin2Xiang-Yue Chen3Xiao-Qing Ma4Bao-Hong Wei5Jing-Guang Lu6Hong-Bing Liu7Key Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaKey Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaQingdao Institute for Food and Drug Control, Qingdao, ChinaKey Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaKey Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaKey Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaQingdao Institute for Food and Drug Control, Qingdao, ChinaKey Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, ChinaContext Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear.Objective To investigate the antiperspirant effect and underlying mechanisms of MLS.Materials and methods Fifty rats were divided into control, model, and three doses of MLS intervention groups (n = 10). Rats except for control group were induced diseases features of the applicable scope of MLS via i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats via s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-γ, and TNF-α. Rat plasma was collected for endogenous metabolite analysis via UPLC-QE-Focus-MS.Results Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio via increasing IL-2 (38.3%), IFN-γ (20.1%), and TNF-α (22.0%) and decreasing IL-6 (24.7%) compared with the model group (p < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS (p < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism.Conclusions MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect.https://www.tandfonline.com/doi/10.1080/13880209.2022.2074465Traditional Chinese medicinehyperhidrosispharmacodynamicsmetabolic mechanism |
| spellingShingle | Shan-Peng Ma Wei-Ping Ma Shi-Ning Yin Xiang-Yue Chen Xiao-Qing Ma Bao-Hong Wei Jing-Guang Lu Hong-Bing Liu Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics Pharmaceutical Biology Traditional Chinese medicine hyperhidrosis pharmacodynamics metabolic mechanism |
| title | Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics |
| title_full | Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics |
| title_fullStr | Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics |
| title_full_unstemmed | Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics |
| title_short | Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics |
| title_sort | antiperspirant effects and mechanism investigation of mulisan decoction in rats based on plasma metabolomics |
| topic | Traditional Chinese medicine hyperhidrosis pharmacodynamics metabolic mechanism |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2022.2074465 |
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