Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine
ABSTRACT The objective of this study was to investigate the effects of 2% L-glutamine supplementation on myenteric innervation in the ileum of diabetic rats, grouped as follows: normoglycemic (N); normoglycemic supplemented with L-glutamine (NG); diabetic (D); and diabetic supplemented with L-glutam...
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| Language: | English |
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Academia Brasileira de Ciências
2016-01-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200609&lng=en&tlng=en |
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| author | RENATA V.F. PEREIRA DAVID R. LINDEN MARCÍLIO H. MIRANDA-NETO JACQUELINE N. ZANONI |
| author_facet | RENATA V.F. PEREIRA DAVID R. LINDEN MARCÍLIO H. MIRANDA-NETO JACQUELINE N. ZANONI |
| author_sort | RENATA V.F. PEREIRA |
| collection | DOAJ |
| description | ABSTRACT The objective of this study was to investigate the effects of 2% L-glutamine supplementation on myenteric innervation in the ileum of diabetic rats, grouped as follows: normoglycemic (N); normoglycemic supplemented with L-glutamine (NG); diabetic (D); and diabetic supplemented with L-glutamine (DG). The ileums were subjected to immunohistochemical techniques to localize neurons immunoreactive to HuC/D protein (HuC/D-IR) and neuronal nitric oxide synthase enzyme (nNOS-IR) and to analyze varicosities immunoreactive to vasoactive intestinal polypeptide (VIP-IR) and calcitonin gene-related peptide (CGRP-IR). L-Glutamine in the DG group (i) prevented the increase in the cell body area of nNOS-IR neurons, (ii) prevented the increase in the area of VIP-IR varicosities, (iii) did not prevent the loss of HuC/D-IR and nNOS-IR neurons per ganglion, and (iv) reduced the size of CGRP-IR varicosities. L-Glutamine in the NG group reduced (i) the number of HuC/D-IR and nNOS-IR neurons per ganglion, (ii) the cell body area of nNOS-IR neurons, and (iii) the size of VIP-IR and CGRP-IR varicosities. 2% L-glutamine supplementation exerted differential neuroprotective effects in experimental diabetes neuropathy that depended on the type of neurotransmitter analyzed. However, the effects of this dose of L-glutamine on normoglycemic animals suggests there are additional actions of this beyond its antioxidant capacity. |
| first_indexed | 2024-04-13T13:30:33Z |
| format | Article |
| id | doaj.art-85df931cc7ca42e7ac7a2d37f38a419f |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-13T13:30:33Z |
| publishDate | 2016-01-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-85df931cc7ca42e7ac7a2d37f38a419f2022-12-22T02:44:59ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902016-01-0188suppl 160962210.1590/0001-3765201620150228S0001-37652016000200609Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamineRENATA V.F. PEREIRADAVID R. LINDENMARCÍLIO H. MIRANDA-NETOJACQUELINE N. ZANONIABSTRACT The objective of this study was to investigate the effects of 2% L-glutamine supplementation on myenteric innervation in the ileum of diabetic rats, grouped as follows: normoglycemic (N); normoglycemic supplemented with L-glutamine (NG); diabetic (D); and diabetic supplemented with L-glutamine (DG). The ileums were subjected to immunohistochemical techniques to localize neurons immunoreactive to HuC/D protein (HuC/D-IR) and neuronal nitric oxide synthase enzyme (nNOS-IR) and to analyze varicosities immunoreactive to vasoactive intestinal polypeptide (VIP-IR) and calcitonin gene-related peptide (CGRP-IR). L-Glutamine in the DG group (i) prevented the increase in the cell body area of nNOS-IR neurons, (ii) prevented the increase in the area of VIP-IR varicosities, (iii) did not prevent the loss of HuC/D-IR and nNOS-IR neurons per ganglion, and (iv) reduced the size of CGRP-IR varicosities. L-Glutamine in the NG group reduced (i) the number of HuC/D-IR and nNOS-IR neurons per ganglion, (ii) the cell body area of nNOS-IR neurons, and (iii) the size of VIP-IR and CGRP-IR varicosities. 2% L-glutamine supplementation exerted differential neuroprotective effects in experimental diabetes neuropathy that depended on the type of neurotransmitter analyzed. However, the effects of this dose of L-glutamine on normoglycemic animals suggests there are additional actions of this beyond its antioxidant capacity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200609&lng=en&tlng=enNeuropatia diabéticaGlutaminaÍleoInervação mioentérica |
| spellingShingle | RENATA V.F. PEREIRA DAVID R. LINDEN MARCÍLIO H. MIRANDA-NETO JACQUELINE N. ZANONI Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine Anais da Academia Brasileira de Ciências Neuropatia diabética Glutamina Íleo Inervação mioentérica |
| title | Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine |
| title_full | Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine |
| title_fullStr | Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine |
| title_full_unstemmed | Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine |
| title_short | Differential effects in CGRPergic, nitrergic, and VIPergic myenteric innervation in diabetic rats supplemented with 2% L-glutamine |
| title_sort | differential effects in cgrpergic nitrergic and vipergic myenteric innervation in diabetic rats supplemented with 2 l glutamine |
| topic | Neuropatia diabética Glutamina Íleo Inervação mioentérica |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200609&lng=en&tlng=en |
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