Effect of glycyrrhizin and its derivatives on integrity of human red blood cells
Introduction: The first and most prevailing cells that glycyrrhizin (GL) and glycyrrhetinic acid (GA) encounter are red blood cells (RBCs). However, what follows this event is poorly understood. This study aims to evaluate the effect of GL and its derivatives on the integrity of human RBCs. Methods:...
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| Format: | Article |
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Shahrekord University of Medical Sciences
2022-10-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-11-546.pdf |
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| author | Diyor Fayziev Petr Merzlyak Sarvinoz Rustamova Ozoda Khamidova Ranokhon Kurbannazarova Ravshan Sabirov |
| author_facet | Diyor Fayziev Petr Merzlyak Sarvinoz Rustamova Ozoda Khamidova Ranokhon Kurbannazarova Ravshan Sabirov |
| author_sort | Diyor Fayziev |
| collection | DOAJ |
| description | Introduction: The first and most prevailing cells that glycyrrhizin (GL) and glycyrrhetinic acid (GA) encounter are red blood cells (RBCs). However, what follows this event is poorly understood. This study aims to evaluate the effect of GL and its derivatives on the integrity of human RBCs. Methods: The integrity of human RBC was assessed under normal isotonic conditions and following osmotic and nystatin-induced colloid-osmotic stress by measuring the amount of hemoglobin released. The pore size was determined by the osmotic protection method. Results: GL was found to be virtually non-hemolytic. However, removal of the carbohydrate moiety of GL imparted significant RBC lytic activity to the cis-(beta-) but not to the trans-(alpha-) isoform of GA. The hemisuccinate radical at position C3 (carbenoxolone) greatly diminished the hemolytic property of GA. The RBC lysis occurred by colloid-osmotic mechanism due to the formation of hydrophilic pores with the radius of ~2.3 nm. At the sublytic doses, the two stereo-isoforms displayed opposite effects on the osmo-resistivity of human RBC: osmoprotection for alpha-GA and osmotic sensibilization for beta-GA. Similar osmotic sensibilization was also observed for GL and carbenoxolone. The two stereo-isoforms exhibited different but not opposite weakening effects on the resistivity of the RBC to the colloid-osmotic stress induced by nystatin, a pore-former. The weakening effect was found intermediate for GL and absent for carbenoxolone. Conclusion: Upon intestinal digestion and absorption, depending on the structure and dosage, the GL hydrolysis products interact with RBC with both beneficial and detrimental consequences. |
| first_indexed | 2024-04-13T15:33:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-04-13T15:33:09Z |
| publishDate | 2022-10-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-85e181559467438980074db9813b86dd2022-12-22T02:41:19ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042022-10-0111454655310.34172/jhp.2022.63jhp-44455Effect of glycyrrhizin and its derivatives on integrity of human red blood cellsDiyor Fayziev0Petr Merzlyak1Sarvinoz Rustamova2Ozoda Khamidova3Ranokhon Kurbannazarova4Ravshan Sabirov5Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, UzbekistanInstitute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, UzbekistanInstitute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, UzbekistanInstitute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, UzbekistanInstitute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, UzbekistanInstitute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, UzbekistanIntroduction: The first and most prevailing cells that glycyrrhizin (GL) and glycyrrhetinic acid (GA) encounter are red blood cells (RBCs). However, what follows this event is poorly understood. This study aims to evaluate the effect of GL and its derivatives on the integrity of human RBCs. Methods: The integrity of human RBC was assessed under normal isotonic conditions and following osmotic and nystatin-induced colloid-osmotic stress by measuring the amount of hemoglobin released. The pore size was determined by the osmotic protection method. Results: GL was found to be virtually non-hemolytic. However, removal of the carbohydrate moiety of GL imparted significant RBC lytic activity to the cis-(beta-) but not to the trans-(alpha-) isoform of GA. The hemisuccinate radical at position C3 (carbenoxolone) greatly diminished the hemolytic property of GA. The RBC lysis occurred by colloid-osmotic mechanism due to the formation of hydrophilic pores with the radius of ~2.3 nm. At the sublytic doses, the two stereo-isoforms displayed opposite effects on the osmo-resistivity of human RBC: osmoprotection for alpha-GA and osmotic sensibilization for beta-GA. Similar osmotic sensibilization was also observed for GL and carbenoxolone. The two stereo-isoforms exhibited different but not opposite weakening effects on the resistivity of the RBC to the colloid-osmotic stress induced by nystatin, a pore-former. The weakening effect was found intermediate for GL and absent for carbenoxolone. Conclusion: Upon intestinal digestion and absorption, depending on the structure and dosage, the GL hydrolysis products interact with RBC with both beneficial and detrimental consequences.http://herbmedpharmacol.com/PDF/jhp-11-546.pdfglycyrrhizinglycyrrhetinic acidcarbenoxoloneerythrocytesred blood cell lysispore size |
| spellingShingle | Diyor Fayziev Petr Merzlyak Sarvinoz Rustamova Ozoda Khamidova Ranokhon Kurbannazarova Ravshan Sabirov Effect of glycyrrhizin and its derivatives on integrity of human red blood cells Journal of HerbMed Pharmacology glycyrrhizin glycyrrhetinic acid carbenoxolone erythrocytes red blood cell lysis pore size |
| title | Effect of glycyrrhizin and its derivatives on integrity of human red blood cells |
| title_full | Effect of glycyrrhizin and its derivatives on integrity of human red blood cells |
| title_fullStr | Effect of glycyrrhizin and its derivatives on integrity of human red blood cells |
| title_full_unstemmed | Effect of glycyrrhizin and its derivatives on integrity of human red blood cells |
| title_short | Effect of glycyrrhizin and its derivatives on integrity of human red blood cells |
| title_sort | effect of glycyrrhizin and its derivatives on integrity of human red blood cells |
| topic | glycyrrhizin glycyrrhetinic acid carbenoxolone erythrocytes red blood cell lysis pore size |
| url | http://herbmedpharmacol.com/PDF/jhp-11-546.pdf |
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