Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones.
BACKGROUND: Nucleation of cholesterol monohydrate crystals following the aggregation and fusion of cholesterol-enriched vesicles is a critical procedure in the formation of cholesterol gallstone. Biliary proteins play important roles in the process. It is inefficient to screen pro-nucleating or anti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3547929?pdf=render |
| _version_ | 1819149047036903424 |
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| author | Diancai Zhang Jianbin Xiang Liying Wang Zhibin Xu Lidong Sun Feng Zhou Xiliang Zha Duan Cai |
| author_facet | Diancai Zhang Jianbin Xiang Liying Wang Zhibin Xu Lidong Sun Feng Zhou Xiliang Zha Duan Cai |
| author_sort | Diancai Zhang |
| collection | DOAJ |
| description | BACKGROUND: Nucleation of cholesterol monohydrate crystals following the aggregation and fusion of cholesterol-enriched vesicles is a critical procedure in the formation of cholesterol gallstone. Biliary proteins play important roles in the process. It is inefficient to screen pro-nucleating or anti-nucleating proteins with routine physiochemical techniques, by which we discovered several pro-nucleating proteins. METHODOLOGY/PRINCIPAL FINDINGS: Based on comparative proteomic technologies, we investigated the differentially expressed proteins between the cholesterol gallstone and control groups, and between the vesicular phase and micellar phase. There are 401±75 spots detected on the cholesterol gallstone group and 389±94 spots on the control group gels, 120±24 spots detected on vesicular phase and 198±37 on micellar phase gels, and accordingly 22 and 8 differentially expressed proteins were identified successfully, respectively. Three of them, HSA, Profilin and Retinol Binding Protein, were validated by Western blot. CONCLUSION/SIGNIFICANCE: Some of the identified proteins are in good agreement with proteins reported to be involved in the gallstone formation before. The information from this study might provide some important clues to uncover the key proteins involved in the formation of cholesterol gallstone. |
| first_indexed | 2024-12-22T13:55:23Z |
| format | Article |
| id | doaj.art-85e3ddcdcddb4973ab1d646a24213086 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:55:23Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-85e3ddcdcddb4973ab1d646a242130862022-12-21T18:23:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5448910.1371/journal.pone.0054489Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones.Diancai ZhangJianbin XiangLiying WangZhibin XuLidong SunFeng ZhouXiliang ZhaDuan CaiBACKGROUND: Nucleation of cholesterol monohydrate crystals following the aggregation and fusion of cholesterol-enriched vesicles is a critical procedure in the formation of cholesterol gallstone. Biliary proteins play important roles in the process. It is inefficient to screen pro-nucleating or anti-nucleating proteins with routine physiochemical techniques, by which we discovered several pro-nucleating proteins. METHODOLOGY/PRINCIPAL FINDINGS: Based on comparative proteomic technologies, we investigated the differentially expressed proteins between the cholesterol gallstone and control groups, and between the vesicular phase and micellar phase. There are 401±75 spots detected on the cholesterol gallstone group and 389±94 spots on the control group gels, 120±24 spots detected on vesicular phase and 198±37 on micellar phase gels, and accordingly 22 and 8 differentially expressed proteins were identified successfully, respectively. Three of them, HSA, Profilin and Retinol Binding Protein, were validated by Western blot. CONCLUSION/SIGNIFICANCE: Some of the identified proteins are in good agreement with proteins reported to be involved in the gallstone formation before. The information from this study might provide some important clues to uncover the key proteins involved in the formation of cholesterol gallstone.http://europepmc.org/articles/PMC3547929?pdf=render |
| spellingShingle | Diancai Zhang Jianbin Xiang Liying Wang Zhibin Xu Lidong Sun Feng Zhou Xiliang Zha Duan Cai Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones. PLoS ONE |
| title | Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones. |
| title_full | Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones. |
| title_fullStr | Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones. |
| title_full_unstemmed | Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones. |
| title_short | Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones. |
| title_sort | comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones |
| url | http://europepmc.org/articles/PMC3547929?pdf=render |
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