In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees

The influence of tree age, explant source and genotype on micropropagation of mature trees of Sorbus aucuparia has been investigated. Experiments demonstrated the feasibility to use juvenile parts of mature trees for in vitro propagation of selected genotypes. Explants from lower branches and from e...

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Main Author: V. Chalupa
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2002-12-01
Series:Journal of Forest Science
Subjects:
Online Access:https://jfs.agriculturejournals.cz/artkey/jfs-200212-0002_in-vitro-propagation-of-mature-trees-of-sorbus-aucuparia-l-and-field-performance-of-micropropagated-trees.php
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author V. Chalupa
author_facet V. Chalupa
author_sort V. Chalupa
collection DOAJ
description The influence of tree age, explant source and genotype on micropropagation of mature trees of Sorbus aucuparia has been investigated. Experiments demonstrated the feasibility to use juvenile parts of mature trees for in vitro propagation of selected genotypes. Explants from lower branches and from epicormic shoots of mature trees exhibited high multiplication coefficients of microshoots cultured on modified MS agar nutrient medium supplemented with cytokinin (BA, TDZ) and auxin (IBA). Microshoots produced from juvenile parts of mature trees exhibited good rooting response and produced plants were well adapted to grow in forest soils. The survival of micropropagated trees planted in experimental plots was high and losses during winter were low. Height and diameter increments of micropropagated trees originated from juvenile parts of mature trees were considerable and their dimensions after five years of growth were comparable with the dimensions of trees originated from seeds.
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spelling doaj.art-85e5737caac14b61a1bc9d1fa13c78af2023-02-23T03:41:25ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2002-12-01481252953510.17221/11923-JFSjfs-200212-0002In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated treesV. Chalupa0Czech University of Agriculture, Faculty of Forestry, Prague, Czech RepublicThe influence of tree age, explant source and genotype on micropropagation of mature trees of Sorbus aucuparia has been investigated. Experiments demonstrated the feasibility to use juvenile parts of mature trees for in vitro propagation of selected genotypes. Explants from lower branches and from epicormic shoots of mature trees exhibited high multiplication coefficients of microshoots cultured on modified MS agar nutrient medium supplemented with cytokinin (BA, TDZ) and auxin (IBA). Microshoots produced from juvenile parts of mature trees exhibited good rooting response and produced plants were well adapted to grow in forest soils. The survival of micropropagated trees planted in experimental plots was high and losses during winter were low. Height and diameter increments of micropropagated trees originated from juvenile parts of mature trees were considerable and their dimensions after five years of growth were comparable with the dimensions of trees originated from seeds.https://jfs.agriculturejournals.cz/artkey/jfs-200212-0002_in-vitro-propagation-of-mature-trees-of-sorbus-aucuparia-l-and-field-performance-of-micropropagated-trees.phpin vitro propagationsorbus aucuparia l.mature tree micropropagationjuvenile parts of treesfield growth of micropropagated trees
spellingShingle V. Chalupa
In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees
Journal of Forest Science
in vitro propagation
sorbus aucuparia l.
mature tree micropropagation
juvenile parts of trees
field growth of micropropagated trees
title In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees
title_full In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees
title_fullStr In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees
title_full_unstemmed In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees
title_short In vitro propagation of mature trees of Sorbus aucuparia L. and field performance of micropropagated trees
title_sort in vitro propagation of mature trees of sorbus aucuparia l and field performance of micropropagated trees
topic in vitro propagation
sorbus aucuparia l.
mature tree micropropagation
juvenile parts of trees
field growth of micropropagated trees
url https://jfs.agriculturejournals.cz/artkey/jfs-200212-0002_in-vitro-propagation-of-mature-trees-of-sorbus-aucuparia-l-and-field-performance-of-micropropagated-trees.php
work_keys_str_mv AT vchalupa invitropropagationofmaturetreesofsorbusaucuparialandfieldperformanceofmicropropagatedtrees