Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern
Abstract Background Delays in diagnosing multidrug-resistant tuberculosis (MDR-TB) are responsible for higher tuberculosis morbidity and mortality and its subsequent transmission. Molecular assays such as real-time PCR (RT-PCR) used to identify drug resistance in Mycobacterium tuberculosis are more...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-11-01
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| Series: | The Egyptian Journal of Bronchology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.4103/1687-8426.217631 |
| _version_ | 1818048231187152896 |
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| author | Gamal Rabie Agamy Safaa Moktar Wafy Asmaa Omar Ahmed Lamees Mohamed Bakar |
| author_facet | Gamal Rabie Agamy Safaa Moktar Wafy Asmaa Omar Ahmed Lamees Mohamed Bakar |
| author_sort | Gamal Rabie Agamy |
| collection | DOAJ |
| description | Abstract Background Delays in diagnosing multidrug-resistant tuberculosis (MDR-TB) are responsible for higher tuberculosis morbidity and mortality and its subsequent transmission. Molecular assays such as real-time PCR (RT-PCR) used to identify drug resistance in Mycobacterium tuberculosis are more rapid than standard drug susceptibility testing. Objectives The aim of this study was to evaluate the diagnostic performance of the Anyplex MTB/MDR RT-PCR assay in detecting MDR-TB strains. Patients and methods Sputum samples were collected from 29 patients with symptoms and radiological findings suggestive of active pulmonary tuberculosis, with at least one of three sputum smear samples showing acid-fast bacilli and/ or sputum culture isolates positive for M. tuberculosis. The results obtained by RT-PCR were compared with those obtained by the Mycobacterium growth indicator tube SIRE method. Results M. tuberculosis was confirmed in 29 specimens. Only six cases determined as MDR-TB were obtained by Mycobacterium growth indicator tube SIRE. For detection of rifampicin-resistant and isoniazid-resistant strains, the RT-PCR assay yielded a sensitivity of 62.5 and 66.66% and specificity of 80 and 95%, respectively. The overall sensitivity of that assay was 64.2% and specificity was 88.88%. Conclusion RT-PCR is an easy and reliable assay for rapid detection of MDR-TB in clinical specimens. However, RT-PCR should be followed by a culture method to increase the overall sensitivity of that assay. |
| first_indexed | 2024-12-10T10:18:24Z |
| format | Article |
| id | doaj.art-85e67f9771cc4660b6bc85e48a90956a |
| institution | Directory Open Access Journal |
| issn | 1687-8426 2314-8551 |
| language | English |
| last_indexed | 2024-12-10T10:18:24Z |
| publishDate | 2017-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | The Egyptian Journal of Bronchology |
| spelling | doaj.art-85e67f9771cc4660b6bc85e48a90956a2022-12-22T01:52:56ZengSpringerOpenThe Egyptian Journal of Bronchology1687-84262314-85512017-11-0111434234510.4103/1687-8426.217631Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concernGamal Rabie Agamy0Safaa Moktar Wafy1Asmaa Omar Ahmed2Lamees Mohamed Bakar3ERS National Delegate of Egypt, Assuit UniversityFellow at Chest Diseases and Tuberculosis Department, Faculty of Medicine, Assuit University HospitalMicrobiology and Clinical Pathology Department, Faculty of Medicine, Assuit UniversityFellow at Chest Diseases and Tuberculosis Department, Faculty of Medicine, Assuit University HospitalAbstract Background Delays in diagnosing multidrug-resistant tuberculosis (MDR-TB) are responsible for higher tuberculosis morbidity and mortality and its subsequent transmission. Molecular assays such as real-time PCR (RT-PCR) used to identify drug resistance in Mycobacterium tuberculosis are more rapid than standard drug susceptibility testing. Objectives The aim of this study was to evaluate the diagnostic performance of the Anyplex MTB/MDR RT-PCR assay in detecting MDR-TB strains. Patients and methods Sputum samples were collected from 29 patients with symptoms and radiological findings suggestive of active pulmonary tuberculosis, with at least one of three sputum smear samples showing acid-fast bacilli and/ or sputum culture isolates positive for M. tuberculosis. The results obtained by RT-PCR were compared with those obtained by the Mycobacterium growth indicator tube SIRE method. Results M. tuberculosis was confirmed in 29 specimens. Only six cases determined as MDR-TB were obtained by Mycobacterium growth indicator tube SIRE. For detection of rifampicin-resistant and isoniazid-resistant strains, the RT-PCR assay yielded a sensitivity of 62.5 and 66.66% and specificity of 80 and 95%, respectively. The overall sensitivity of that assay was 64.2% and specificity was 88.88%. Conclusion RT-PCR is an easy and reliable assay for rapid detection of MDR-TB in clinical specimens. However, RT-PCR should be followed by a culture method to increase the overall sensitivity of that assay.http://link.springer.com/article/10.4103/1687-8426.217631isoniazidmultidrug-resistant tuberculosismycobacterium growth indicator tubereal-time polymerase chain reactionrifampicin |
| spellingShingle | Gamal Rabie Agamy Safaa Moktar Wafy Asmaa Omar Ahmed Lamees Mohamed Bakar Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern The Egyptian Journal of Bronchology isoniazid multidrug-resistant tuberculosis mycobacterium growth indicator tube real-time polymerase chain reaction rifampicin |
| title | Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern |
| title_full | Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern |
| title_fullStr | Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern |
| title_full_unstemmed | Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern |
| title_short | Real-time polymerase chain reaction as an alternative method for diagnosis of multidrug-resistant tuberculosis: can it stand alone in this concern |
| title_sort | real time polymerase chain reaction as an alternative method for diagnosis of multidrug resistant tuberculosis can it stand alone in this concern |
| topic | isoniazid multidrug-resistant tuberculosis mycobacterium growth indicator tube real-time polymerase chain reaction rifampicin |
| url | http://link.springer.com/article/10.4103/1687-8426.217631 |
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