Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides
Insecticides not only control target pests but also adversely affect non-target communities including humans, animals, and microbial communities in host plants and soils. The effect of insecticides on non-target communities, especially endophytic bacterial communities, remains poorly understood. Two...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15306 |
| _version_ | 1797573633069023232 |
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| author | Md. Tareq Bin Salam Ryota Kataoka |
| author_facet | Md. Tareq Bin Salam Ryota Kataoka |
| author_sort | Md. Tareq Bin Salam |
| collection | DOAJ |
| description | Insecticides not only control target pests but also adversely affect non-target communities including humans, animals, and microbial communities in host plants and soils. The effect of insecticides on non-target communities, especially endophytic bacterial communities, remains poorly understood. Two phases of treatments were conducted to compare the trends in endophytic bacterial response after insecticide application. Endophytic bacteria were isolated at 2 and 4 weeks after germination. Most insecticide treatments showed a declining trend in bacterial diversity and abundance, whereas an increasing trend was observed in the control. Therefore, insecticide use negatively affected non-target endophytic bacterial communities. <i>Bacillus</i> spp. was mostly dominant in the early stage in both insecticide treatment and control groups. Nevertheless, in the matured stage, mostly bacteria including <i>Pseudomonas</i> spp., <i>Priestia</i> spp. were dominant in groups treated with high insecticide concentrations. Therefore, plants can regulate and moderate their microbiome during their lifecycle depending on surrounding environmental conditions. |
| first_indexed | 2024-03-10T21:11:52Z |
| format | Article |
| id | doaj.art-85e7a83af8f449bc9a4e91403691f220 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:11:52Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-85e7a83af8f449bc9a4e91403691f2202023-11-19T16:44:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201530610.3390/ijms242015306Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic InsecticidesMd. Tareq Bin Salam0Ryota Kataoka1Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu 400-8510, Yamanashi, JapanFaculty of Life and Environmental Sciences, University of Yamanashi, Kofu 400-8510, Yamanashi, JapanInsecticides not only control target pests but also adversely affect non-target communities including humans, animals, and microbial communities in host plants and soils. The effect of insecticides on non-target communities, especially endophytic bacterial communities, remains poorly understood. Two phases of treatments were conducted to compare the trends in endophytic bacterial response after insecticide application. Endophytic bacteria were isolated at 2 and 4 weeks after germination. Most insecticide treatments showed a declining trend in bacterial diversity and abundance, whereas an increasing trend was observed in the control. Therefore, insecticide use negatively affected non-target endophytic bacterial communities. <i>Bacillus</i> spp. was mostly dominant in the early stage in both insecticide treatment and control groups. Nevertheless, in the matured stage, mostly bacteria including <i>Pseudomonas</i> spp., <i>Priestia</i> spp. were dominant in groups treated with high insecticide concentrations. Therefore, plants can regulate and moderate their microbiome during their lifecycle depending on surrounding environmental conditions.https://www.mdpi.com/1422-0067/24/20/15306endophytic bacteriainsecticidespolymerase chain reactionrestriction fragment length polymorphismbacterial diversityprincipal component analysis |
| spellingShingle | Md. Tareq Bin Salam Ryota Kataoka Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides International Journal of Molecular Sciences endophytic bacteria insecticides polymerase chain reaction restriction fragment length polymorphism bacterial diversity principal component analysis |
| title | Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides |
| title_full | Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides |
| title_fullStr | Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides |
| title_full_unstemmed | Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides |
| title_short | Changes in the Endophytic Bacterial Community of <i>Brassica rapa</i> after Application of Systemic Insecticides |
| title_sort | changes in the endophytic bacterial community of i brassica rapa i after application of systemic insecticides |
| topic | endophytic bacteria insecticides polymerase chain reaction restriction fragment length polymorphism bacterial diversity principal component analysis |
| url | https://www.mdpi.com/1422-0067/24/20/15306 |
| work_keys_str_mv | AT mdtareqbinsalam changesintheendophyticbacterialcommunityofibrassicarapaiafterapplicationofsystemicinsecticides AT ryotakataoka changesintheendophyticbacterialcommunityofibrassicarapaiafterapplicationofsystemicinsecticides |