An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells
Abstract Objective To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of São Paulo
2023-06-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100427&lng=en&tlng=en |
| _version_ | 1827932302043250688 |
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| author | Nutthapong KANTRONG Jittranut KUMTAWEE Teerasak DAMRONGRUNGRUANG Subin PUASIRI Anupong MAKEUDOM Suttichai KRISANAPRAKORNKIT Pattama CHAILERTVANITKUL |
| author_facet | Nutthapong KANTRONG Jittranut KUMTAWEE Teerasak DAMRONGRUNGRUANG Subin PUASIRI Anupong MAKEUDOM Suttichai KRISANAPRAKORNKIT Pattama CHAILERTVANITKUL |
| author_sort | Nutthapong KANTRONG |
| collection | DOAJ |
| description | Abstract Objective To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells. Methodology Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. Results Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. Conclusions Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties. |
| first_indexed | 2024-03-13T07:07:44Z |
| format | Article |
| id | doaj.art-85e9cb4dbea84967afec69d27ec2582f |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-03-13T07:07:44Z |
| publishDate | 2023-06-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-85e9cb4dbea84967afec69d27ec2582f2023-06-06T07:35:32ZengUniversity of São PauloJournal of Applied Oral Science1678-77652023-06-013110.1590/1678-7757-2023-0006An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cellsNutthapong KANTRONGhttps://orcid.org/0000-0001-9365-2929Jittranut KUMTAWEEhttps://orcid.org/0000-0002-3110-2822Teerasak DAMRONGRUNGRUANGhttps://orcid.org/0000-0003-4795-6221Subin PUASIRIhttps://orcid.org/0000-0002-9562-6254Anupong MAKEUDOMhttps://orcid.org/0000-0002-1490-2342Suttichai KRISANAPRAKORNKIThttps://orcid.org/0000-0001-9189-0333Pattama CHAILERTVANITKULhttps://orcid.org/0000-0002-6520-6750Abstract Objective To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells. Methodology Dental pulp cells, isolated from three freshly extracted third molars, were first characterized for their mesenchymal origin and treated with 10 ng/ml of IL-1β in the presence or absence of non-toxic concentrations of the extract from 0.08 to 1.25 mg/ml, as determined by the PrestoBlue cytotoxic assay. Total RNA was harvested and analyzed for mRNA expressions of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2). Western blot hybridization was performed to investigate COX-2 protein expression. Culture supernatants were assayed for released prostaglandin E2 levels. Immunofluorescence was conducted to determine involvement of nuclear factor-kappaB (NF-kB) in the inhibitory effect of the extract. Results Stimulation of the pulp cells with IL-1β resulted in the activation of arachidonic acid metabolism via COX-2, but not 5-LOX. Incubation with various non-toxic concentrations of the propolis extract significantly inhibited upregulated COX-2 mRNA and protein expressions upon treatment with IL-1β (p<0.05), resulting in a significant decrease in elevated PGE2 levels (p<0.05). Nuclear translocation of the p50 and the p65 subunits of NF-kB upon treatment with IL-1β was also blocked by incubation with the extract. Conclusions Upregulated COX-2 expression and enhanced PGE2 synthesis upon treatment with IL-1β in human dental pulp cells were suppressed by incubation with non-toxic doses of Thai propolis extract via involvement of the NF-kB activation. This extract could be therapeutically used as a pulp capping material due to its anti-inflammatory properties.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100427&lng=en&tlng=enCyclooxygenase-2Dental pulp cellsInterleukin-1PropolisProstaglandin E2 |
| spellingShingle | Nutthapong KANTRONG Jittranut KUMTAWEE Teerasak DAMRONGRUNGRUANG Subin PUASIRI Anupong MAKEUDOM Suttichai KRISANAPRAKORNKIT Pattama CHAILERTVANITKUL An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells Journal of Applied Oral Science Cyclooxygenase-2 Dental pulp cells Interleukin-1 Propolis Prostaglandin E2 |
| title | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
| title_full | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
| title_fullStr | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
| title_full_unstemmed | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
| title_short | An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells |
| title_sort | in vitro anti inflammatory effect of thai propolis in human dental pulp cells |
| topic | Cyclooxygenase-2 Dental pulp cells Interleukin-1 Propolis Prostaglandin E2 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572023000100427&lng=en&tlng=en |
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