Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37
A crude extract (B10) of Aspergillus niger spores showed effective antibacterial activity against Agrobacterium tumefaciens T-37. The inhibition rate was 98.22%, and the half maximal inhibitory concentration was 0.035 ± 0.018 mg/mL. To study the primary antibacterial mechanism, this study used relat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2020.1858722 |
| _version_ | 1818427608249925632 |
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| author | Kun Guo Qinyu Zhang Jinyi Zhao Zhu Li Jiang Ran Yang Xiao Su Zhang Mianmian Hu |
| author_facet | Kun Guo Qinyu Zhang Jinyi Zhao Zhu Li Jiang Ran Yang Xiao Su Zhang Mianmian Hu |
| author_sort | Kun Guo |
| collection | DOAJ |
| description | A crude extract (B10) of Aspergillus niger spores showed effective antibacterial activity against Agrobacterium tumefaciens T-37. The inhibition rate was 98.22%, and the half maximal inhibitory concentration was 0.035 ± 0.018 mg/mL. To study the primary antibacterial mechanism, this study used relative electric conductivity, release of proteins and nucleic acids, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) and detection of reactive oxygen species (ROS). After adding B10, the relative electric conductivity increased in the supernatant indicating electrolyte transport from intracellular to extracellular space. Compared with the control group and the benzylpenicillin potassium (BP) group, the B10 group had a significant increase in extracellular nucleic acid and protein within 0–18 h, showing that the cell and cytoplasmic membranes were damaged by B10. The SDS–PAGE results confirmed that the extracellular protein and nucleic acid levels were in agreement with lower intracellular total protein. Finally, the intracellular levels of ROS indicated that B10 caused an increase in ROS. In summary, B10 showed clear antibacterial activity against A. tumefaciens through damaging cytoplasmic membranes. The research provided a scientific basis for the prevention and treatment of bacteria. |
| first_indexed | 2024-12-14T14:48:26Z |
| format | Article |
| id | doaj.art-85ea4502fb574d8b88742f57965e9bdf |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-14T14:48:26Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-85ea4502fb574d8b88742f57965e9bdf2022-12-21T22:57:13ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302021-01-0135116216910.1080/13102818.2020.18587221858722Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37Kun Guo0Qinyu Zhang1Jinyi Zhao2Zhu Li3Jiang Ran4Yang Xiao5Su Zhang6Mianmian Hu7Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityGuizhou Bureau of Quality and Technical SupervisionKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou UniversityA crude extract (B10) of Aspergillus niger spores showed effective antibacterial activity against Agrobacterium tumefaciens T-37. The inhibition rate was 98.22%, and the half maximal inhibitory concentration was 0.035 ± 0.018 mg/mL. To study the primary antibacterial mechanism, this study used relative electric conductivity, release of proteins and nucleic acids, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) and detection of reactive oxygen species (ROS). After adding B10, the relative electric conductivity increased in the supernatant indicating electrolyte transport from intracellular to extracellular space. Compared with the control group and the benzylpenicillin potassium (BP) group, the B10 group had a significant increase in extracellular nucleic acid and protein within 0–18 h, showing that the cell and cytoplasmic membranes were damaged by B10. The SDS–PAGE results confirmed that the extracellular protein and nucleic acid levels were in agreement with lower intracellular total protein. Finally, the intracellular levels of ROS indicated that B10 caused an increase in ROS. In summary, B10 showed clear antibacterial activity against A. tumefaciens through damaging cytoplasmic membranes. The research provided a scientific basis for the prevention and treatment of bacteria.http://dx.doi.org/10.1080/13102818.2020.1858722aspergillus nigercrude extractsantibacterial mechanismagrobacterium tumefaciens |
| spellingShingle | Kun Guo Qinyu Zhang Jinyi Zhao Zhu Li Jiang Ran Yang Xiao Su Zhang Mianmian Hu Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 Biotechnology & Biotechnological Equipment aspergillus niger crude extracts antibacterial mechanism agrobacterium tumefaciens |
| title | Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 |
| title_full | Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 |
| title_fullStr | Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 |
| title_full_unstemmed | Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 |
| title_short | Antibacterial mechanism of Aspergillus niger xj spore powder crude extract B10 against Agrobacterium tumefaciens T-37 |
| title_sort | antibacterial mechanism of aspergillus niger xj spore powder crude extract b10 against agrobacterium tumefaciens t 37 |
| topic | aspergillus niger crude extracts antibacterial mechanism agrobacterium tumefaciens |
| url | http://dx.doi.org/10.1080/13102818.2020.1858722 |
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