Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle
Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) anti...
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| Format: | Article |
| Language: | English |
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Urmia University
2014-06-01
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| Series: | Veterinary Research Forum |
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| Online Access: | http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-5%20no-2/141-144-1099.pdf |
| _version_ | 1811301753865371648 |
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| author | Jafar Arjmand Bijan Esmaeilnejad Mohammad Hossein Razi Jalali Masoud Ghorbanpoor Seyyed Meysam Abtahi |
| author_facet | Jafar Arjmand Bijan Esmaeilnejad Mohammad Hossein Razi Jalali Masoud Ghorbanpoor Seyyed Meysam Abtahi |
| author_sort | Jafar Arjmand |
| collection | DOAJ |
| description | Fasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this purpose, the sera specimens of slaughtered cattle were taken and examined for fasciola infection. Sera from two groups of cattle, one infected with fasciola (n = 60) and the other non-infected with fasciola (n = 60), were used in the Dot- ELISA test. All sera were tested and evaluated. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of Dot- ELISA with ES Ag were better than those of Dot- ELISA with Cr Ag. In conclusion, excretory–secretory antigen dependent Dot-ELISA can be used as a reliable sero-diagnostic test for fasciola infection in cattle. |
| first_indexed | 2024-04-13T07:14:41Z |
| format | Article |
| id | doaj.art-85ed019b03fe4e36aa90de466cd20681 |
| institution | Directory Open Access Journal |
| issn | 2008-8140 |
| language | English |
| last_indexed | 2024-04-13T07:14:41Z |
| publishDate | 2014-06-01 |
| publisher | Urmia University |
| record_format | Article |
| series | Veterinary Research Forum |
| spelling | doaj.art-85ed019b03fe4e36aa90de466cd206812022-12-22T02:56:47ZengUrmia UniversityVeterinary Research Forum2008-81402014-06-0152141144Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattleJafar Arjmand0Bijan Esmaeilnejad1Mohammad Hossein Razi Jalali2Masoud Ghorbanpoor3Seyyed Meysam Abtahi 4Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Ahvaz University, Ahvaz, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Ahvaz University, Ahvaz, IranDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranFasciolosis is a disease caused by liver fluck of the genus of fasciola. Diagnosis of fasciolosis has been challenging for a long period due to low sensitivity of the coprological diagnostic method. In this study, an in-house Dot-ELISA method; using excretion–secretory (ES Ag) and Crude (Cr Ag) antigens of fasciola was described for diagnosis of fasciolosis in cattle. For this purpose, the sera specimens of slaughtered cattle were taken and examined for fasciola infection. Sera from two groups of cattle, one infected with fasciola (n = 60) and the other non-infected with fasciola (n = 60), were used in the Dot- ELISA test. All sera were tested and evaluated. Except specificity, other parameters such as, sensitivity, accuracy, positive and negative predictive values of Dot- ELISA with ES Ag were better than those of Dot- ELISA with Cr Ag. In conclusion, excretory–secretory antigen dependent Dot-ELISA can be used as a reliable sero-diagnostic test for fasciola infection in cattle.http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-5%20no-2/141-144-1099.pdfCattle; Dot-ELISA; Fasciolosis |
| spellingShingle | Jafar Arjmand Bijan Esmaeilnejad Mohammad Hossein Razi Jalali Masoud Ghorbanpoor Seyyed Meysam Abtahi Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle Veterinary Research Forum Cattle; Dot-ELISA; Fasciolosis |
| title | Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle |
| title_full | Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle |
| title_fullStr | Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle |
| title_full_unstemmed | Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle |
| title_short | Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle |
| title_sort | designing and evaluation of dot elisa for diagnosis of fasciola infection in cattle |
| topic | Cattle; Dot-ELISA; Fasciolosis |
| url | http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-5%20no-2/141-144-1099.pdf |
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