Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor
Bagasse as solid waste in sugarcane industry can be utilized as one of the potential raw materials in the bioprocess industry. This research aims to investigate the conversion of bagasse to bioethanol using simultaneous saccharification and fermentation in an airlift bioreactor. Neurospora intermedi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Diponegoro University
2020-07-01
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| Series: | International Journal of Renewable Energy Development |
| Subjects: | |
| Online Access: | https://ijred.cbiore.id/index.php/ijred/article/view/26433 |
| _version_ | 1797449842720505856 |
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| author | Elvi Restiawaty Kindi Pyta Gani Arinta Dewi Linea Alfa Arina Katarina Ika Kurniawati Yogi Wibisono Budhi Akhmaloka Akhmaloka |
| author_facet | Elvi Restiawaty Kindi Pyta Gani Arinta Dewi Linea Alfa Arina Katarina Ika Kurniawati Yogi Wibisono Budhi Akhmaloka Akhmaloka |
| author_sort | Elvi Restiawaty |
| collection | DOAJ |
| description | Bagasse as solid waste in sugarcane industry can be utilized as one of the potential raw materials in the bioprocess industry. This research aims to investigate the conversion of bagasse to bioethanol using simultaneous saccharification and fermentation in an airlift bioreactor. Neurospora intermedia was used as a biological agent that carried out the saccharification and fermentation of sugarcane bagasse simultaneously for bioethanol production. Cell morphology of N. intermedia in the form of pellet was required to provide free movement in the axial flow of airlift bioreactor. The medium pH strongly affects the morphological shape of N. intermedia. Therefore, the formation of good pellets of inoculum was observed under acidic conditions, i.e. pH 3.0 – 3.5. The effect of the initial concentration of nutrient on the inoculum growth was also investigated. Inoculums cultured in potato dextrose broth (PDB) medium with a half the strength of the common nutrient concentration of PDB qualitatively indicated good growth in terms of the size and density of cells. The inoculums with good morphological form were fed into the airlift bioreactor, which already contained a liquid medium with initial pH of 3.5 and also contained pre-treated bagasse. In experiments using the airlift bioreactor, the pre-treated bagasse was added to various nutrient concentrations of the PDB infusion medium. The highest bioethanol production from bagasse was monitored in the medium culture of half strength PDB infusion. The yield of bioethanol obtained from total sugarcane bagasse and PDB in an air lift bioreactor achieved approximately 40%, which has an infusion medium with a half-strength PDB and initial pH of 3.0. |
| first_indexed | 2024-03-09T14:30:53Z |
| format | Article |
| id | doaj.art-85eea1b1181f43fb90e572c039164e68 |
| institution | Directory Open Access Journal |
| issn | 2252-4940 |
| language | English |
| last_indexed | 2024-03-09T14:30:53Z |
| publishDate | 2020-07-01 |
| publisher | Diponegoro University |
| record_format | Article |
| series | International Journal of Renewable Energy Development |
| spelling | doaj.art-85eea1b1181f43fb90e572c039164e682023-11-28T02:08:35ZengDiponegoro UniversityInternational Journal of Renewable Energy Development2252-49402020-07-019224725310.14710/ijred.9.2.247-25316210Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift BioreactorElvi Restiawaty0https://orcid.org/0000-0002-2127-3898Kindi Pyta Gani1Arinta Dewi2Linea Alfa Arina3Katarina Ika Kurniawati4Yogi Wibisono Budhi5Akhmaloka Akhmaloka61. Research Group of Chemical Engineering Process Design and Development, Faculty of Industrial Technology, Institut Teknologi Bandung, Indonesia, IndonesiaBiosciences and Biotechnology Research Center, Institut Teknologi Bandung, Indonesia, IndonesiaBiosciences and Biotechnology Research Center, Institut Teknologi Bandung, Indonesia, IndonesiaBiosciences and Biotechnology Research Center, Institut Teknologi Bandung, Indonesia, IndonesiaBiosciences and Biotechnology Research Center, Institut Teknologi Bandung, Indonesia, IndonesiaResearch Group of Chemical Engineering Process Design and Development, Faculty of Industrial Technology, Institut Teknologi Bandung, Indonesia, IndonesiaResearch Group of Biochemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia, IndonesiaBagasse as solid waste in sugarcane industry can be utilized as one of the potential raw materials in the bioprocess industry. This research aims to investigate the conversion of bagasse to bioethanol using simultaneous saccharification and fermentation in an airlift bioreactor. Neurospora intermedia was used as a biological agent that carried out the saccharification and fermentation of sugarcane bagasse simultaneously for bioethanol production. Cell morphology of N. intermedia in the form of pellet was required to provide free movement in the axial flow of airlift bioreactor. The medium pH strongly affects the morphological shape of N. intermedia. Therefore, the formation of good pellets of inoculum was observed under acidic conditions, i.e. pH 3.0 – 3.5. The effect of the initial concentration of nutrient on the inoculum growth was also investigated. Inoculums cultured in potato dextrose broth (PDB) medium with a half the strength of the common nutrient concentration of PDB qualitatively indicated good growth in terms of the size and density of cells. The inoculums with good morphological form were fed into the airlift bioreactor, which already contained a liquid medium with initial pH of 3.5 and also contained pre-treated bagasse. In experiments using the airlift bioreactor, the pre-treated bagasse was added to various nutrient concentrations of the PDB infusion medium. The highest bioethanol production from bagasse was monitored in the medium culture of half strength PDB infusion. The yield of bioethanol obtained from total sugarcane bagasse and PDB in an air lift bioreactor achieved approximately 40%, which has an infusion medium with a half-strength PDB and initial pH of 3.0.https://ijred.cbiore.id/index.php/ijred/article/view/26433bioethanolairlift bioreactorsugarcane bagasseneurospora intermediapellet |
| spellingShingle | Elvi Restiawaty Kindi Pyta Gani Arinta Dewi Linea Alfa Arina Katarina Ika Kurniawati Yogi Wibisono Budhi Akhmaloka Akhmaloka Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor International Journal of Renewable Energy Development bioethanol airlift bioreactor sugarcane bagasse neurospora intermedia pellet |
| title | Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor |
| title_full | Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor |
| title_fullStr | Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor |
| title_full_unstemmed | Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor |
| title_short | Bioethanol Production from Sugarcane Bagasse Using Neurospora intermedia in an Airlift Bioreactor |
| title_sort | bioethanol production from sugarcane bagasse using neurospora intermedia in an airlift bioreactor |
| topic | bioethanol airlift bioreactor sugarcane bagasse neurospora intermedia pellet |
| url | https://ijred.cbiore.id/index.php/ijred/article/view/26433 |
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