Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle
Antibiotic resistance represents a growing crisis in both human and veterinary medicine. We evaluated the use of antibiotic alternatives—heavy metals and essential oils—in beef cattle feeding, and their effects on Gram-negative and Gram-positive bacteria. In this randomized controlled field trial, w...
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MDPI AG
2021-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/2/259 |
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| author | Sarah A. Murray Raghavendra G. Amachawadi Keri N. Norman Sara D. Lawhon Tiruvoor G. Nagaraja James S. Drouillard Harvey M. Scott |
| author_facet | Sarah A. Murray Raghavendra G. Amachawadi Keri N. Norman Sara D. Lawhon Tiruvoor G. Nagaraja James S. Drouillard Harvey M. Scott |
| author_sort | Sarah A. Murray |
| collection | DOAJ |
| description | Antibiotic resistance represents a growing crisis in both human and veterinary medicine. We evaluated the use of antibiotic alternatives—heavy metals and essential oils—in beef cattle feeding, and their effects on Gram-negative and Gram-positive bacteria. In this randomized controlled field trial, we measured the impact of supplemental zinc and menthol on antibiotic resistance among commensal enteric bacteria of feeder cattle. Fecal suspensions were plated onto plain- and antibiotic-supplemented MacConkey and m-<i>Enterococcus</i> agar for quantification of total and antibiotic-resistant <i>Escherichia coli</i> and <i>Enterococcus</i> spp., respectively. Temporal effects on overall <i>E. coli</i> growth were significant (<i>p</i> < 0.05), and menthol was associated with decreased growth on tetracycline-supplemented agar. Zinc was associated with significant increases in growth on erythromycin-supplemented m-<i>Enterococcus</i> agar. Cattle fed zinc exhibited significantly higher levels of macrolide resistance among fecal enterococci isolates. |
| first_indexed | 2024-03-09T04:07:50Z |
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| id | doaj.art-85f0c9738e72492c8699b5acb12111f5 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T04:07:50Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-85f0c9738e72492c8699b5acb12111f52023-12-03T14:04:18ZengMDPI AGAnimals2076-26152021-01-0111225910.3390/ani11020259Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef CattleSarah A. Murray0Raghavendra G. Amachawadi1Keri N. Norman2Sara D. Lawhon3Tiruvoor G. Nagaraja4James S. Drouillard5Harvey M. Scott6Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USADepartment of Clinical Sciences, Kansas State University, Manhattan, KS 66506, USADepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USADepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USADepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, USADepartment of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, USADepartment of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843, USAAntibiotic resistance represents a growing crisis in both human and veterinary medicine. We evaluated the use of antibiotic alternatives—heavy metals and essential oils—in beef cattle feeding, and their effects on Gram-negative and Gram-positive bacteria. In this randomized controlled field trial, we measured the impact of supplemental zinc and menthol on antibiotic resistance among commensal enteric bacteria of feeder cattle. Fecal suspensions were plated onto plain- and antibiotic-supplemented MacConkey and m-<i>Enterococcus</i> agar for quantification of total and antibiotic-resistant <i>Escherichia coli</i> and <i>Enterococcus</i> spp., respectively. Temporal effects on overall <i>E. coli</i> growth were significant (<i>p</i> < 0.05), and menthol was associated with decreased growth on tetracycline-supplemented agar. Zinc was associated with significant increases in growth on erythromycin-supplemented m-<i>Enterococcus</i> agar. Cattle fed zinc exhibited significantly higher levels of macrolide resistance among fecal enterococci isolates.https://www.mdpi.com/2076-2615/11/2/259antibiotic resistanceantibiotic alternativesheavy metalsessential oils |
| spellingShingle | Sarah A. Murray Raghavendra G. Amachawadi Keri N. Norman Sara D. Lawhon Tiruvoor G. Nagaraja James S. Drouillard Harvey M. Scott Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle Animals antibiotic resistance antibiotic alternatives heavy metals essential oils |
| title | Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle |
| title_full | Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle |
| title_fullStr | Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle |
| title_full_unstemmed | Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle |
| title_short | Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among <i>E. coli</i> and <i>Enterococcus</i> spp. in Beef Cattle |
| title_sort | effects of zinc and menthol based diets on co selection of antibiotic resistance among i e coli i and i enterococcus i spp in beef cattle |
| topic | antibiotic resistance antibiotic alternatives heavy metals essential oils |
| url | https://www.mdpi.com/2076-2615/11/2/259 |
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